Identification and Expression Analysis of Tea Plant (Camellia sinesis) miR164a and Its Target Gene

KONG Lei; ZHU Xiangxiang; WANG Yiwei; XIE Xiaofang; JIANG Changjun; LI Yeyun

doi:10.13305/j.cnki.jts.2018.06.001

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Journal of Tea Science ›› 2018, Vol. 38 ›› Issue (6) : 547-558. DOI: 10.13305/j.cnki.jts.2018.06.001

Identification and Expression Analysis of Tea Plant (Camellia sinesis) miR164a and Its Target Gene

KONG Lei, ZHU Xiangxiang, WANG Yiwei, XIE Xiaofang, JIANG Changjun, LI Yeyun^*

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Abstract

Plant miR164 is involved in complex physiological processes such as plant growth and development regulation and stress responses by negatively regulating the target gene NAC transcription factors. The identification, cloning and sequence analysis of miR164a and its target gene NAC1 in tea plant were carried out by high-throughput sequencing of microRNA and degradome analysis. The expression patterns of Csn-miR164a and its target gene CsNAC1 during leaf development and under high or low temperature stresses were also studied. The length of Csn-miR164a precursor is 126βbp, and the mature sequence is 21βbp. The CsNAC1 ORF is 912βbp, encoding 303 amino acids. There are a typical NAM domain at the N terminus of CsNAC1 protein and a Csn-miR164a recognition site at the C-terminus. Quantitative real-time PCR analysis showed that the expression of Csn-miR164a was the highest in the bud and the lowest in the seventh leaves. Under high and low temperature treatments, the expression of Csn-miR164a was down regulated, while the expression level of CsNAC1 increased. The expression of Csn-miR164a was negatively correlatied with the expression of the target gene CsNAC1. The results revealed that Csn-miR164a and its target gene CsNAC1 might be closely related to the growth and stress responses in tea plant.

Key words

Camellia sinensis / miR164a / NAC / target gene / temperature stress

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KONG Lei, ZHU Xiangxiang, WANG Yiwei, XIE Xiaofang, JIANG Changjun, LI Yeyun. Identification and Expression Analysis of Tea Plant (Camellia sinesis) miR164a and Its Target Gene[J]. Journal of Tea Science. 2018, 38(6): 547-558 https://doi.org/10.13305/j.cnki.jts.2018.06.001

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