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Journal of Tea Science ›› 2019, Vol. 39 ›› Issue (6): 681-691.doi: 10.13305/j.cnki.jts.2019.06.007

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Screening of miRNA Related to Anthocyanin Synthesis in Tea Cultivar ‘Zijuan’ Based on High Throughput Sequencing

CHEN Linbo1,2, XIA Lifei1, LIU Yue1, SUN Yunnan1, JIANG Huibing1, TIAN Yiping1, CHEN Liang2,*   

  1. 1. Tea Research Institute, Yunnan Academy of Agricultural Sciences/Yunnan Engineering Research Centerof Tea Germplasm Innovation and Matching Cultivation /Yunnan Provincial Key Laboratory of Tea Science, Menghai 666201, China;
    2. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Received:2019-03-04 Revised:2019-05-25 Online:2019-12-15 Published:2019-12-24

Abstract: In order to screen the miRNAs related to anthocyanin synthesis in 'Zijuan', the miRNA library was constructed by using the tea cultivars 'Zijuan' (ZJ), 'Yunkang 10' (YK) and 'Fuding Dabaicha' (FD). In this study, 46 known miRNAs and 67 unknown miRNAs were identified, and 765 annotated target genes were predicted. A total of 24 miRNAs were screened out, which were differentially expressed between ZJ and YK, and between ZJ and FD. Four target miRNAs involved in the regulation of anthocyanin synthesis, namely miR828a, miR845c, novel_14 and novel_87, were identified by target gene analysis of 24 differentially expressed miRNAs. The predicted target genes include transcription factor genes MYB4, MYB23, MYB26, MYB82, bHLH74 and 4CL (4-coincyl-CoA-linked enzyme), DFR (dihydroflavonol 4-reductase) and UFGT (UDP-glucoside flavonoid 3-O-glucosyltransferase). Eight differentially expressed miRNAs were analyzed by RT-PCR and the results were consistent with transcriptome analysis. Finally, a theoretical basis for further study of regulation mechanisms related to anthocyanin biosynthesis in tea plant was provided.

Key words: Camellia sinensis, anthocyanin synthesis, high throughput sequencing, microRNAs, target genes

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