Absorption and Accumulation Characteristics of Selenium in Tea Plant (Camellia sinensis) and Expression Analysis of Genes Related to Selenium Regulation

CAO Dan; MA Linlong; LIU Yanli; GONG Ziming; JIN Xiaofang

doi:10.13305/j.cnki.jts.20191202.002

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Journal of Tea Science ›› 2020, Vol. 40 ›› Issue (1) : 77-84. DOI: 10.13305/j.cnki.jts.20191202.002

Absorption and Accumulation Characteristics of Selenium in Tea Plant (Camellia sinensis) and Expression Analysis of Genes Related to Selenium Regulation

CAO Dan, MA Linlong, LIU Yanli, GONG Ziming, JIN Xiaofang^*

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Abstract

The concentration dependence and time course of selenium (Se) absorption and accumulation in tea plant were investigated under sand culture and the expressions of related genes were also analyzed. The results show that the absorption amounts of Se in different tissues of tea plant were remarkably different. Most of them were fixed by the roots, and low movement from roots to shoots was observed. Moreover, the accumulation of Se was significantly correlated with exogenous Se concentration and culture duration. Furthermore, tea plant grew well in the Se concentration from 0 to 0.05 mmol·L^-1, but when the concentration was higher than 0.10 mmol·L^-1, tea plant showed poisoning symptoms. Fluorescence quantitative PCR analysis indicates that allene oxide cyclase (CsAOC), lipoxygenase (CsLOX6) (key genes of jasmonic acid signaling pathway), as well as a basic helix-loop-helix transcription factor (CsbHLH62) could be obviously induced by high concentration of Se. The correlation analysis showes that the expressions of these genes were positively related with the Se content in roots. These results suggest that Se accumulation in different tissues of tea plant was significantly correlated with exogenous Se concentration and culture duration, and CsbHLH62, CsAOC, CsLOX6 might play important roles in this process.

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absorption and accumulation / gene expression / Se / tea plant

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CAO Dan, MA Linlong, LIU Yanli, GONG Ziming, JIN Xiaofang. Absorption and Accumulation Characteristics of Selenium in Tea Plant (Camellia sinensis) and Expression Analysis of Genes Related to Selenium Regulation[J]. Journal of Tea Science. 2020, 40(1): 77-84 https://doi.org/10.13305/j.cnki.jts.20191202.002

References

[1] 王子健. 中国低硒带生态环境中硒的环境行为研究进展[J]. 环境化学, 1993(3): 237-243.
Wang Z J.Some biogeochemical aspects of selenium in Chinese low-selenium belt[J]. Environmental Chemistry, 1993(3): 237-243.
[2] Brooks J D, Metter E J, Chan D W, et al.Plasma selenium level before diagnosis and the risk of prostate cancer development[J]. Journal of Urology, 2001, 166(6): 2034-2038.
[3] Hawrylaknowak B.Beneficial effects of exogenous selenium in cucumber seedlings subjected to salt stress[J]. Biological Trace Element Research, 2009, 132(1/3): 259-269.
[4] Cartes P, Jara A A, Pinilla L, et al.Selenium improves the antioxidant ability against aluminium-induced oxidative stress in ryegrass roots[J]. Annals of Applied Biology, 2010, 156(2): 297-307.
[5] Mimmo T, Tiziani R, Valentinuzzi F, et al.Selenium biofortification in Fragaria×ananassa: implications on strawberry fruits quality, content of bioactive health beneficial compounds and metabolomic profile[J]. Frontiers in Plant Science, 2017, 8: 1887. doi: 10.3389/fpls.2017.01887.
[6] 叶飞, 龚自明, 高士伟, 等. 湖北恩施茶园土壤及茶叶硒元素调查研究[J]. 四川农业大学学报, 2015, 33(3): 275-278.
Ye F, Gong Z M, Gao S W, et al.Investigation of the selenium element in tea plantation of Enshi District, Hubei Province[J]. Journal of Sichuan Agricultural University, 2015, 33(3): 275-278.
[7] 刘海燕, 黄彩梅, 周盛勇, 等. 茶园土壤及与茶叶中微量元素锌硒含量相关性的研究[J]. 广西植物, 2015, 35(6): 868-874, 941.
Liu H Y, Huang C M, Zhou S Y, et al.Correlation of zinc and selenium contents in tea and the planting soil[J]. Guihaia, 2015, 35(6): 868-874, 941.
[8] 周超, 胡玉荣, 曾建明, 等. 土壤因子对茶树硒吸收特性的影响[J]. 茶叶科学, 2015, 35(5): 429-436.
Zhou C, Hu Y R, Zeng J M, et al.Effects of soil factors on the selenium absorption characteristics of tea plant[J]. Journal of Tea Science, 2015, 35(5): 429-436.
[9] Cao D, Liu Y, Ma L, et al.Transcriptome analysis of differentially expressed genes involved in selenium accumulation in tea plant (Camellia sinensis)[J]. PLoS ONE, 2018, 13(6): e0197506. doi: 10.1371/journal.pone.0197506.
[10] 金孝芳, 贾尚智, 闵彩云, 等. 茶树良种‘金茗1号’[J]. 林业科学, 2015, 51(12): 156-156.
Jin X F, Jia S Z, Min C Y, et al.An Elite Variety of Camellia sinensis 'Jinming 1'[J]. Scientia Silvae Sinicae, 2015, 51(12): 156-156.
[11] Liu Y, Cao D, Ma L, et al.TMT-based quantitative proteomics analysis reveals the response of Camellia sinensis, to fluoride[J]. Journal of Proteomics, 2018, 176: 71-81.
[12] 中华全国供销合作总社. 茶叶中硒含量的检测方法: GB/T 21729—2008[S]. 北京: 中国标准出版社, 2008.
All China Federation of Supply and Marketing Cooperatives. Determination of selenium content in tea: GB/T 21729—2008 [S]. Beijing: Standards Press of China, 2008.
[13] 明佳佳, 胡承孝, 赵小虎, 等. 硒对油菜各部位矿质元素含量及其迁移特征的影响[J]. 浙江农业学报, 2016, 28(9): 1564-1571.
Ming J J, Hu C X, Zhao X H, et al.Content and migration characteristic of mineral element in rape with application of selenium[J]. Acta Agriculturae Zhejiangensis, 2016, 28(9): 1564-1571.
[14] 王磊, 黄婷婷, 杨春, 等. 叶面喷硒对台茶12号所制红绿茶含硒量及品质的影响[J]. 西南农业学报, 2016, 29(11): 2578-2582.
Wang L, Huang T T, Yang C, et al.Effect of selenium content and quality of black tea and green tea made of taiwan tea 12 by foliar spray of selenium[J]. Southwest China Journal of Agricultural Sciences, 2016, 29(11): 2578-2582.
[15] 杨海滨, 李中林, 徐泽, 等. 施肥对富硒茶园茶叶硒含量、养分和品质的影响[J]. 中国农业科技导报, 2018, 20(5): 130-137.
Yang H B, Li Z L, Xu Z, et al.Effects of fertilization on selenium content, nutrient and quality of tea in se-enriched tea garden[J]. Journal of Agricultural Science and Technology, 2018, 20(5): 130-137.
[16] Bañuelos G S, Lin Z Q.Phytoremediation management of selenium-laden drainage sediments in the San Luis Drain: a greenhouse feasibility study[J]. Ecotoxicology & Environmental Safety, 2005, 62(3): 309-316.
[17] Eustice D C, Foster I, Kull F J, et al.In vitro incorporation of selenomethionine into protein by Vigna radiata polysomes[J]. Plant Physiology, 1980, 66(1): 182-186.
[18] Elrashidi M A, Adriano D C, Workman S M, et al.Chemical equilibria of selenium insoils: a theoretical development[J]. Soil Science, 1987, 144(2): 274-280.
[19] 张均华, 朱练峰, 禹盛苗, 等. 稻田硒循环转化与水稻硒营养研究进展[J]. 应用生态学报, 2012, 23(10): 2900-2906.
Zhang J H, Zhu L F, Yu S M, et al.Selenium cycling and transformation in paddy field and selenium nutrition of rice: A review[J]. Chinese Journal of Applied Ecology, 2012, 23(10): 2900-2906.
[20] 段曼莉, 付冬冬, 王松山, 等. 亚硒酸盐对四种蔬菜生长、吸收及转运硒的影响[J]. 环境科学学报, 2011, 31(3): 658-665.
Duan M L, Fu D D, Wang S S, et al.Effects of different selenite concentrations on plant growth, absorption and transportation of selenium in four different vegetables[J]. Acta Scientiae Circumstantiae, 2011, 31(3): 658-665.
[21] 徐云, 王子健, 王文华, 等. Se和环境中富里酸对小麦种子发芽的影响及其生理特性[J]. 应用生态学报, 1997(4): 439-444.
Xu Y, Wang Z J, Wang W H, et al.Effect of selenium and fulvic acid on seed germination of wheat and its physiological properties[J]. Chinese Journal of Applied Ecology, 1997(4): 439-444.
[22] 周鑫斌, 施卫明, 杨林章. 富硒与非富硒水稻品种对硒的吸收分配的差异及机理[J]. 土壤, 2007, 39(5): 731-736.
Zhou X B, Shi W M, Yang L Z.Genotypical differences and characteristics of se uptake and accumulation in rice[J]. Soils, 2007, 39(5): 731-736.
[23] Wang X F, Zhang L, Wan Y N, et al.Effects of foliar-applied selenite and selenate on selenium accumulation in strawberry[J]. Journal of Agricultural Resources and Environment, 2016, 33(4): 334-339.
[24] Chen S Y, Jiang R F, Li H F.Uptake and translocation of selenate or selenite by wheat and rice seedlings[J]. Environmental Science, 2011, 32(1): 284-289.
[25] Liu X W, Zhao Z Q, Hu C X, et al.Effect of sulphate on selenium uptake and translocation in rape (Brassica napus L.) supplied with selenate or selenite[J]. Plant and Soil, 2016, 399(1/2): 295-304.
[26] Anjum S A, Wang L, Farooq M, et al.Methyl jasmonate-induced alteration in lipid peroxidation, antioxidative defence system and yield in soybean under drought[J]. Journal of Agronomy & Crop Science, 2011, 197(4): 296-301.
[27] 曹丹, 金孝芳, 马林龙, 等. 基于RNA-seq技术的茶树CsbHLH62生物信息学分析[J]. 西南农业学报, 2019, 32(1): 36-41.
Cao D, Jin X F, Ma L L, et al.Bioinformatics analysis of CsbHLH62 in tea plant based on rna sequencing[J]. Southwest China Journal of Agricultural Sciences, 2019, 32(1): 36-41.
[28] Wu H L, Chen C L, Du J, et al.Co-Overexpression FIT with AtbHLH38 or AtbHLH39 in Arabidopsis-Enhanced cadmium tolerance via increased cadmium sequestration in roots and improved iron homeostasis of shoots[J]. Plant Physiology, 2012, 158(2): 790-800.
[29] Mortel J E V D, Schat H, Moerland P D, et al. Expression differences for genes involved in lignin, glutathione and sulphate metabolism in response to cadmium in Arabidopsis thaliana and the related Zn/Cd-hyperaccumulator Thlaspi caerulescens[J]. Plant Cell & Environment, 2010, 31(3): 301-324.
[30] 廖永翠. 茉莉酸信号途径参与调控沉香倍半萜生物合成的分子机制研究[D]. 北京: 北京协和医学院, 2015.
Liao Y C.Moleular mechanism of JA signaling pathway involved in the regulation of agarwood sesquiterpene biosynthesis [D]. Beijing: Peking Union Medical College, 2015.
[31] Zhao Y, Zhou L M, Chen Y Y, et al.MYC genes with differential responses to tapping, mechanical wounding, ethrel and methyl jasmonate in laticifers of rubber tree (Hevea brasiliensis Muell. Arg.)[J]. Journal of Plant Physiology, 2011, 168(14): 1649-1658.
[32] Tamaoki M, Freeman J L, Pilon-Smits E A H. Cooperative ethylene and jasmonic acid signaling regulates selenite resistance in Arabidopsis[J]. Plant Physiology, 2008, 146(3): 1219-1230.
[33] Wang J, Cappa J J, Harris J P, et al.Transcriptome-wide comparison of selenium hyperaccumulator and non-accumulator, Stanleya, species provides new insight into key processes mediating the hyperaccumulation syndrome[J]. Plant Biotechnology Journal, 2018, 16(9): 1582-1594.
[34] 李欣, 李影, 曲子越, 等. bHLH转录因子在茉莉酸信号诱导植物次生产物合成中的作用及分子机制[J]. 植物生理学报, 2017, 53(1): 1-8.
Li X, Li Y, Qu Z Y, et al.The molecular mechanism and the function of bHLH regulating jasmonic acidmediated secondary metabolites synthesis[J]. Plant Physiology Journal, 2017, 53(1): 1-8.