Cloning and Expression Analysis of a Full Length cDNA of CsPAL3 Gene in Tea Plant (Camellia sinensis)

TANG Xiuhua; ZHOU Zhe; TANG Qin; CHEN Jiajia; XIE Feng; HONG Yaoxin; HUANG Shilin; CHEN Zhidan; SUN Weijiang

doi:10.13305/j.cnki.jts.2018.01.004

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Journal of Tea Science ›› 2018, Vol. 38 ›› Issue (1) : 33-42. DOI: 10.13305/j.cnki.jts.2018.01.004

Cloning and Expression Analysis of a Full Length cDNA of CsPAL3 Gene in Tea Plant (Camellia sinensis)

TANG Xiuhua^1,3,4, ZHOU Zhe^1,3,4, TANG Qin^1,3,4, CHEN Jiajia^1,3,4, XIE Feng^1,3,4, HONG Yaoxin^1,3,4, HUANG Shilin², CHEN Zhidan^2,3,4,*, SUN Weijiang^1,2,3,4,*

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Abstract

The phenylalanine ammonia-lyase (PAL) belongs to a multi-gene family. PAL is the first enzyme in the flavonoid biosynthetic pathway, which plays a key role in regulating flavonoid biosynthesis. The full length cDNA sequence of one phenylalanine ammonia-lyase (CsPAL) gene was obtained from purple tea cultivar Wuyi Qizhong C18 (Camellia sinensis) by rapid amplification of cDNA ends PCR(RACE-PCR) and named as CsPAL3 (GenBank accession no. KY865305). The full-length cDNA of CsPAL3 was 2β518βbp, with an ORF of 2β130βbp, which encodes a protein of 709 amino acids. Bioinformatics analysis showed it is a stable hydrophilic protein, with the predict with the predict molecular and theoretic isoelectric points of 77.40 kD and 6.26. Blast analysis indicated that CsPAL3 had the highest similarity (87%) with homologue gene in Mangifera indica,and had the closest genetic relationship with homologue gene in Paeonia lactiflora according to phylogenetic tree analysis. The total anthocyanin contents and the expression of CsPALa, CsPALc, CsPAL3 in the purple shoots of tea plants were significantly higher than the green and white tea plants. It suggests that the enhanced expression levels of CsPALa, CsPALc, CsPAL3 genes might promote the accumulation of anthocyanins, thereby lead to the purple color in tea plants.

Key words

anthocyanins / cloning / CsPAL3 gene / relative expression / tea plant

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TANG Xiuhua, ZHOU Zhe, TANG Qin, CHEN Jiajia, XIE Feng, HONG Yaoxin, HUANG Shilin, CHEN Zhidan, SUN Weijiang. Cloning and Expression Analysis of a Full Length cDNA of CsPAL3 Gene in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science. 2018, 38(1): 33-42 https://doi.org/10.13305/j.cnki.jts.2018.01.004

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