茶刺蛾核型多角体病毒与其它杆状病毒关系的基因分析

doi:10.13305/j.cnki.jts.2005.01.012

茶叶科学 ›› 2005, Vol. 25 ›› Issue (1): 65-70.doi: 10.13305/j.cnki.jts.2005.01.012

茶刺蛾核型多角体病毒与其它杆状病毒关系的基因分析

杨丽荣¹, 肖强², 冷杨², 徐海君¹, 章亦卿¹, 张传溪^{1, *}

1. 浙江大学应用昆虫学研究所,浙江杭州 310029;
2. 中国农业科学院茶叶研究所,浙江杭州 310008

出版日期:2005-03-25 发布日期:2019-09-16
通讯作者: chaxzhang@zju.edu.cn
作者简介:杨丽荣（1976— ）,女,蒙古族,在读博士研究生,研究方向：昆虫分子生物学。
基金资助:
国家自然科学基金资助项目(30070032)和高等学校全国优秀博士学位论文作者专项资金(200055)

Analysis of AcORF47 Homologue and VP80 Gene from a Newly Isolated Nucleopolyhedrovirus from the Tea Slug Moth, Iragoides fasciata

YANG Li-rong¹, XIAO Qiang², LENG Yang², XU Hai-jun¹, ZHANG Yi-qing¹, ZHANG Chuan-Xi^{1, *}

1.Institute of Applied Entomology, Zhejiang University, Hangzhou 310029, China;
2. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China

Online:2005-03-25 Published:2019-09-16

摘要/Abstract

摘要： 茶刺蛾核型多角体病毒Iragoides fasciata Nucleopolyhedrovirus（IrfaNPV）是新分离的一种杆状病毒。本研究克隆和分析了IrfaNPV的AcORF47 同源基因和VP80基因。AcORF47同源基因与苜蓿丫纹夜蛾多粒包埋型核型多角体病毒（Autographa californica multiple nucleocapsid nucleopolyhedrovirus,MNPV,AcMNPV）的ORF47和家蚕核型多角体病毒（Bombyx mori nucleopolyhedrovirus,BmNPV）的ORF38有42%的同源性,与薄荷灰夜蛾多粒包埋型核型多角体病毒（Rachiplusia ou multiple nucleocapsid nucleopolyhedrovirus,RaouMNPV）的ORF44有40%的同源性。IrfaNPV的VP80 C端222氨基酸与17种NPV特有结构蛋白VP80/P87有25%~58%同源性。在18种VP80/P87分子进化树上,IrfaNPV与AcMNPV、BmNPV和RaouMNPV的亲源关系最为接近。

关键词: 茶刺蛾, 核型多角体病毒, AcORF47同源基因, VP80基因, 分子进化

Abstract: A baculovirus, Iragoides fasciata Nucleopolyhedrovirus（IrfaNPV）was newly isolated from the tea slug moth, Iragoides fasciata. The IrfaNPV AcORF47 homologue and VP80 gene were cloned. Sequence analysis showed IrfaNPV AcORF47 homologue shared 42%, 42% and 40% identities with AcMNPV ORF47, BmNPV ORF38 and RaouMNPV ORF44 in amino acid level, respectively. The 222 amino acid residues in the C-terminal of IrfaNPV VP80 had 25%~58% identities with homologous genes from other 17 NPVs. A phylogenetic tree was constructed based on the VP80/P87 amino acid sequences and the result showed that IrfaNPV was most closely related to AcMNPV, BmNPV and RaouMNPV.

Key words: Iragoides fasciata, Nucleopolyhedrovirus, AcORF47 homologue, Phylogeney, VP80

中图分类号:

杨丽荣, 肖强, 冷杨, 徐海君, 章亦卿, 张传溪. 茶刺蛾核型多角体病毒与其它杆状病毒关系的基因分析[J]. 茶叶科学, 2005, 25(1): 65-70. doi: 10.13305/j.cnki.jts.2005.01.012.

YANG Li-rong, XIAO Qiang, LENG Yang, XU Hai-jun, ZHANG Yi-qing, ZHANG Chuan-Xi. Analysis of AcORF47 Homologue and VP80 Gene from a Newly Isolated Nucleopolyhedrovirus from the Tea Slug Moth, Iragoides fasciata[J]. Journal of Tea Science, 2005, 25(1): 65-70. doi: 10.13305/j.cnki.jts.2005.01.012.

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茶刺蛾核型多角体病毒与其它杆状病毒关系的基因分析

Analysis of AcORF47 Homologue and VP80 Gene from a Newly Isolated Nucleopolyhedrovirus from the Tea Slug Moth, Iragoides fasciata

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