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茶叶科学 ›› 2012, Vol. 32 ›› Issue (4): 331-340.doi: 10.13305/j.cnki.jts.2012.04.009

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茶饼病菌侵染对茶树挥发性物质的影响

张春花1, 单治国1,2, 魏朝霞1, 李家华1, 唐嘉义1*   

  1. 1. 云南农业大学,云南 昆明 650201;
    2. 普洱学院,云南 普洱 665000
  • 收稿日期:2012-02-13 修回日期:2012-04-25 出版日期:2012-08-15 发布日期:2019-09-05
  • 通讯作者: ndjytang@163.com
  • 作者简介:张春花(1984— ),女,云南泸西人,博士研究生,主要从事茶树病害的研究。
  • 基金资助:
    国家科技支撑计划项目(2007BAD58B01)

Effects of Exobasidium vexans Massee Infection on Volatile Components in Tea Plant (Camellia Sinensis var. assamica)

ZHANG Chun-hua1, SHAN Zhi-guo1,2, WEI Zhao-xia1, LI Jia-hua1, TANG Jia-yi1*   

  1. 1. Yunnan Agricultural University, Kunming 650201, China;
    2. Department of Life Science, Pu’er College, Pu’er 665000, China;
  • Received:2012-02-13 Revised:2012-04-25 Online:2012-08-15 Published:2019-09-05

摘要: 采用同时蒸馏萃取和气相色谱-质谱联用(SDE/GC/MS)技术,分别测定了健康及受茶饼病菌侵染鲜叶中挥发性物质,探讨了云南大叶种健康及受茶饼病菌侵染鲜叶中挥发性物质的组成和差异,分析了茶饼病菌侵染对茶树挥发性物质的影响。结果表明:茶树健康叶片中共检测出37种挥发性物质,占总挥发物的80.195%,而受茶饼病菌侵染叶片中共检测出56种挥发性物质,占总挥发物的92.166%;在受茶饼病菌侵染叶片中有13种物质未检测出,有31种新的挥发性物质被检测出;芳樟醇是云南大叶种茶树鲜叶中主要的挥发性物质;受茶饼病菌危害的叶片以含氮含硫化合物、萜烯类、芳香族化合物、绿叶性气体为主要挥发物。

关键词: 茶饼病菌, 侵染, 挥发性物质, 气质联用

Abstract: The aim of this experiment was to analyze the variation of component and relative amount of volatile compounds from tea plant infested by Exobasidium vexans Masseeon (E. vexans). The volatile compounds released from tea plant (Camellia sinensis var. assamica) healthy and diseased were analyzed by SDE/GC/MS. Result showed that a total of 37 volatile compounds were detected from the healthy tea plant, as 80.195% of total compounds identified from the healthy tea plants. A total of 56 volatile compounds, 92.166% of compounds were identified from the infected plants. However, 13 volatile components which was existed in intact tea leaf were not detected and 31 new volatile compounds were in E. vexans infested plants. Linalool was the main volatile compound in intacted Camellia sinensis var. assamica. The kinds of azotic, sulfur, terpenes, aromatic, green leaf volatile were main volatile compounds in tea plants infested by E. vexans.

Key words: Exobasidium vexans massee, infested, volatile compounds, GC/MS

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