为探讨云茶1号茶树品种优异性状的遗传基础,采用PacBio平台进行全长转录组测序,最终获得Polished consensus序列213β389个,预测到CDS有223β120个,检测到195β062个SSR位点。在NR数据库有170β264个同源序列比对到980个物种;有103β124个在KOG数据库得到注释,根据其功能各分为26类;有65β524个得到GO注释分为细胞组分、分子功能及生物学过程等三大类的55个功能组;根据KEGG数据库,105β972个得到了注释,涉及到216个代谢途径分支,包括茶叶品质、活性物质代谢以及抗逆等相关基因等;还预测到隶属于60个转录因子家族的转录因子有5β785个。这些结果为进一步开展云茶1号茶树特异性状基因的标记性引物开发、遗传研究以及品质形成和抗逆机制研究奠定了基础。
Abstract
To explore the genetic basis for important traits, the full-length transcriptome of the ‘Yuncha 1’ (Camellia assamica) was sequenced by using PacBio Platform. A total of 213β389 polished consensus were generated, 223β120 coding sequences were predicted and annotated, and 195β062 SSR loci were found. According to NR databases, 170β264 homologous sequences were mapped to 980 species, 103β124 unigenes were further annotated and grouped into 26 functional categories in KOG databases, 65β524 unigenes were annotated against GO database and divided into cellular component, molecular function and biological process categories with a total of 55 functional groups. KEGG pathway analysis showed that 105β972 unigenes could be broadly classified into 216 metabolism pathways according to their function, and some of them were involved in quality, bioactive substances, and resistance gene, etc. It is also predicted that there were 5β785 transcription factors belonging to 60 transcription factor families. The experimental results will give important data for development of SSRs of specific traits, genetic analysis and studies involved in quality formation and resistence mechanism in tea cultivar ‘Yuncha 1’.
关键词
功能注释 /
基因分析 /
全长转录组 /
云茶1号
Key words
full-length transcriptome /
function annotation /
gene analysis /
tea cultivar‘Yuncha 1’
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基金
国家自然科学基金项目（31660224、31560220、31460216)、云南省人才培养计划项目（2015HB105）
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