茶树CsSnRK2.1、CsSnRK2.2基因的克隆及在非生物胁迫中的响应

doi:10.13305/j.cnki.jts.2018.02.009

茶叶科学 ›› 2018, Vol. 38 ›› Issue (2): 183-192.doi: 10.13305/j.cnki.jts.2018.02.009

茶树CsSnRK2.1、CsSnRK2.2基因的克隆及在非生物胁迫中的响应

张永恒¹, 万思卿¹, 陈江飞¹, 王伟东¹, 周天山¹, 肖斌¹, 杨亚军^1,2,*, 余有本^1,*

1. 西北农林科技大学园艺学院,陕西杨凌 712100;
2. 中国农业科学院茶叶研究所/国家茶树改良中心/农业部茶树生物学与资源利用重点实验室,浙江杭州 310008

收稿日期:2017-06-05 修回日期:2017-08-01 出版日期:2018-04-15 发布日期:2019-08-28
通讯作者: *
作者简介:张永恒,男,硕士研究生,主要从事茶树分子育种研究。
基金资助:
国家茶叶产业技术体系、汉中综合试验站（CARS-23）、陕西省农业专项资金项目（tg2015-099）、中国博士后科学基金面上项目（2016M602873）

Cloning and Expression Analysis of CsSnRK2.1 and CsSnRK2.2 Genes in Tea Plant (Camellia sinensis) under Abiotic Stress

ZHANG Yongheng¹, WANG Siqing¹, CHEN Jiangfei¹, WANG Weidong¹, ZHOU Tianshan¹, XIAO Bin¹, YANG Yajun^1,2,*, YU Youben^1,*

1. College of Horticulture, Northwest A&F University, Yangling 712100, China;
2. Tea Research Institute of Chinese Academy, Agricultural Sciences/National Center for Tea Improvement / Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China

Received:2017-06-05 Revised:2017-08-01 Online:2018-04-15 Published:2019-08-28

摘要/Abstract

摘要： 蔗糖非酵解型蛋白激酶（SnRK）是一类广泛存在于植物体内的丝氨酸/苏氨酸类蛋白激酶,其中SnRK2家族在植物非生物胁迫响应过程中起着十分重要的作用。为了探究茶树SnRK2家族基因响应逆境的分子机制,本研究克隆了两个茶树SnRK2家族基因,并分别命名为CsSnRK2.1（GenBank登录号：MG026837）和CsSnRK2.2（GenBank登录号：MF662805）,生物信息学分析表明CsSnRK2.1和CsSnRK2.2分别编码358个和337个氨基酸,与拟南芥和玉米SnRK2蛋白激酶的同源性很高,均具有保守的ATP结合位点和Ser/Thr激酶活性结构域。在高盐、干旱和ABA胁迫下,CsSnRK2.1均能被诱导表达,且呈现先升后降趋势,而在低温和高温胁迫下表达量变化不大;CsSnRK2.2能强烈响应高盐胁迫,也能被高温诱导上调表达;表明它们可能与茶树抗逆密切相关。

关键词: 茶树, SnRK2s, 克隆, 非生物胁迫

Abstract: Sucrose non-fermenting-1-related protein kinase (SnRK) is a kind of serine/threonine protein kinases widely exist in plants. Among them, members of SnRK2 family play a vital role in plant response to stresses. In order to investigate the molecular mechanisms of Camellia sinensis SnRK2 in response to abiotic stresses, two SnRK2 genes from tea plant were cloned and named as CsSnRK2.1 (GenBank accession code: MG026837) and CsSnRK2.2 (GenBank accession code: MF662805) respectively. Bioinformatics analysis showed that they contained 358 and 337 amino acids respectively, which harbored a conserved ATP binding site and Ser/Thr kinase domain and were highly similar to SnRK2 protein kinase of Arabidopsis thaliana and maize. The expression of CsSnRK2.1 was transiently induced and then decreased by high salinity (100βmmol·L^-1 NaCl), drought (20% PEG6000) and ABA(100βμmol·L^-1) stress, but showed no significant changes under low (4℃) or high temperature treatments (38℃). By contrast, CsSnRK2.2 was strongly induced by high salt and temperature treatments. The results revealed that CsSnRK2.1 and CsSnRK2.2 might be closely related to stress responses in tea plant.

Key words: tea plant (Camellia sinensis), SnRK2s, cloning, abiotic stress

中图分类号:

S571.1
Q52

张永恒, 万思卿, 陈江飞, 王伟东, 周天山, 肖斌, 杨亚军, 余有本. 茶树CsSnRK2.1、CsSnRK2.2基因的克隆及在非生物胁迫中的响应[J]. 茶叶科学, 2018, 38(2): 183-192. doi: 10.13305/j.cnki.jts.2018.02.009.

ZHANG Yongheng, WANG Siqing, CHEN Jiangfei, WANG Weidong, ZHOU Tianshan, XIAO Bin, YANG Yajun, YU Youben. Cloning and Expression Analysis of CsSnRK2.1 and CsSnRK2.2 Genes in Tea Plant (Camellia sinensis) under Abiotic Stress[J]. Journal of Tea Science, 2018, 38(2): 183-192. doi: 10.13305/j.cnki.jts.2018.02.009.

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茶树CsSnRK2.1、CsSnRK2.2基因的克隆及在非生物胁迫中的响应

Cloning and Expression Analysis of CsSnRK2.1 and CsSnRK2.2 Genes in Tea Plant (Camellia sinensis) under Abiotic Stress

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