顶梢和功能叶对茶树全光照弥雾扦插快繁的影响研究

doi:10.13305/j.cnki.jts.2018.05.004

茶叶科学 ›› 2018, Vol. 38 ›› Issue (5): 469-479.doi: 10.13305/j.cnki.jts.2018.05.004

顶梢和功能叶对茶树全光照弥雾扦插快繁的影响研究

任恒泽¹, 向勤锃¹, 赵秀秀¹, 蔡鲁², 张丽霞^1,*

1. 山东农业大学园艺科学与工程学院山东泰安 271018;
2. 山东圣豪植物科技有限公司山东滨州 256600

收稿日期:2017-12-21 修回日期:2018-04-08 出版日期:2018-10-15 发布日期:2019-10-15
通讯作者: *zlx_sdau@163.com
作者简介:任恒泽,男,硕士研究生,主要从事茶树生理生态与栽培技术研究。
基金资助:
山东省农业重大应用技术创新项目（02131969）、山东“双一流”奖补资金资助（515563001）

Effects of Apical -shoots and Functional Leaves on the Fast Propagation of Tea Cuttings under the Condition of Full-illumination and Mist

REN Hengze¹, XIANG Qinzeng¹, ZHAO Xiuxiu¹, CAI Lu², ZHANG Lixia^1,*

1. College of Horticulture Science and Engineering, Shandong Agricultural University, Tai′an 271018, China;
2. Shandong Shenghao Plant Technology Co, Ltd, Binzhou 256600, China

Received:2017-12-21 Revised:2018-04-08 Online:2018-10-15 Published:2019-10-15

摘要/Abstract

摘要： 为进一步缩短茶树无性苗繁育周期,提高无性苗质量,本试验以3年生台茶12号（又名金萱）茶树嫩枝为扦插材料,采用全光照弥雾快繁育苗技术,研究了插穗顶梢保留与摘除、不保留成熟功能叶、保留1~3片成熟功能叶对茶树扦插苗根系和地上部生长的影响,并于扦插前对茶树嫩枝不同部位芽叶的相关生理生化指标进行了测定分析。结果表明,随叶片成熟度增加,可溶性糖和叶绿素含量呈递增趋势。净光合速率也随叶片成熟度的增加呈递增趋势,但以第2片成熟功能叶最高。可溶性蛋白含量随叶片成熟度的增加呈下降趋势,以顶部一芽一叶含量最高。带成熟功能叶插穗成活率和生根率均为100.00%,而不带成熟功能叶的一芽一叶插穗和一芽二叶插穗成活率和生根率均仅为5.00%和23.33%。根系生长以带3片功能叶留梢插穗最好,生根早且根量大,10βd内就有大量白色根点从茎段基部冒出,60%~70%茶苗形成二次根仅需37βd,且地上部新形成的成熟功能叶数也最多,但是新梢高度增长值小于保留顶梢带1片成熟功能叶插穗,基部茎粗增长值小于带2片成熟功能叶插穗。成熟功能叶片数的增加有利于根系生长发育,提高成活率和生根率;保留顶梢插穗的根系和地上部的生长优于去梢插穗。

关键词: 茶树, 嫩枝扦插, 顶梢, 功能叶, 快繁生根, 地上部生长

Abstract: To shorten the breeding cycle of tea clones and improve the seedling quality, twigs of three-year-old Camellia sinensis (L.) O. Kuntze cv. Taicha 12 (Jin Xuan) were used as cuttings materials for propagation under the condition of full-illumination and mist. The effects of apical-shoots and cuttings with one, two or three mature functional leaves on rooting and growth of tea cuttings were studied. The physiological and biochemical analysis of buds and leaves of different positions were performed before the cuttage. The results showed that soluble sugar and chlorophyll content increased with the maturity of leaves increased. The net photosynthetic rate showed a similar trend with its peak in the second functional leaves. The contents of soluble protein exhibited an opposite trend with the leaf maturity and the apical shoot with one bud and tender leaves possessed the highest soluble protein contents. Both the survival and rooting rates of cuttings with mature functional leaves were 100.00%, but those for single-bud cuttings with one or two tender leaves were only 5.00% and 23.33% respectively. The best materials for rooting were the cuttings with apical shoot and three mature functional leaves, which possessed the fastest speed of rooting and had the maximum amount of roots. A large number of roots emerged from the base of stem segment within 10 days and the formation of secondary roots for 60%-70% tea cuttings only required 37 days. Its new formed functional leaves were also more than others. However, the seedling heights of tea cuttings with apical shoot and three mature functional leaves were less than those with one bud and two tender leaves. The increasement of basal stem diameter was also smaller. The formation of new functional leaves facilitated root growth and development, which could also improve survival and rooting rates. The rooting and growth of cuttings with apical shoot were better than cuttings without apical shoot.

Key words: tea, softwood cutting, apical shoot, functional leaves, fast propagation and rooting, aerial growth

中图分类号:

任恒泽, 向勤锃, 赵秀秀, 蔡鲁, 张丽霞. 顶梢和功能叶对茶树全光照弥雾扦插快繁的影响研究[J]. 茶叶科学, 2018, 38(5): 469-479. doi: 10.13305/j.cnki.jts.2018.05.004.

REN Hengze, XIANG Qinzeng, ZHAO Xiuxiu, CAI Lu, ZHANG Lixia. Effects of Apical -shoots and Functional Leaves on the Fast Propagation of Tea Cuttings under the Condition of Full-illumination and Mist[J]. Journal of Tea Science, 2018, 38(5): 469-479. doi: 10.13305/j.cnki.jts.2018.05.004.

参考文献 20

[1]	梁月荣, 刘祖生, 庄晚芳. 茶树插穗发根的解剖学和生物化学研究[J]. 茶叶科学, 1985, 5(1): 19-28.
[2]	王雪萍, 龚自明, 高士伟, 等. ABT1号生根粉对茶树穴盘扦插生根的影响[J]. 湖北农业科学, 2013, 52(22): 5509-5511.
[3]	成浩, 曾建明, 周健, 等. 茶树种苗工厂化快速繁育技术[J]. 茶叶科学, 2007, 27(3): 231-235.
[4]	韩晓阳, 张丽霞, 王日为. 茶树育苗基质的筛选及其理化特性的研究[J]. 山东农业科学, 2010(1): 36-39.
[5]	Teshome M, Endale T, Tesfaye S, et al.Effects of various rooting media on survival and growth of tea (Camellia sinensis L.) stem cuttings at Jimma[J]. Journal of Biology, Agriculture and Healthcare, 2016, 6(3): 149-156.
[6]	Gyana Ranjan Rout.Effect of auxins on adventitious root development from single node cuttings of Camellia sinensis (L.) Kuntze and associated biochemical changes[J]. Plant Growth Regulation, 2006, 48: 111-117.
[7]	姚元涛, 宋鲁彬, 田丽丽, 等. 茶树短穗扦插育苗技术[J]. 落叶果树, 2009, 41(4): 33-35.
[8]	张珊珊, 杨志新, 刘炳光, 等. 茶树良种短穗扦插育苗技术[J]. 中国园艺文摘, 2014, 30(11): 221-222.
[9]	程徽儿, 杨维时. 茶树梢头扦插育苗效果[J]. 茶叶科学, 1995, 15(1): 77-78.
[10]	李传忠. 不同插穗扦插苗质量差异的研究[J]. 茶叶通讯, 2006, 33(3): 19-20.
[11]	赵晓敏, 霍常富, 沈海龙. 影响林木插条生根的内部及环境因子研究综述[J]. 世界林业研究, 2007, 20(5): 12-16.
[12]	张正竹. 茶叶生物化学实验教程[M]. 北京: 中国农业出版社, 2009: 44-45.
[13]	黄惠华, 高孔荣. 茶叶可溶性蛋白质含量的测定及添加PVP的效应研究[J]. 食品科学, 1993, 14(11): 3-6.
[14]	高俊凤. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006: 74-75.
[15]	王峰, 陈玉真, 王秀萍. 茶树不同叶位叶片功能性状与光合特性研究[J]. 茶叶科学, 2016, 36(1): 77-84.
[16]	董雅致, 徐克章, 崔喜艳, 等. 不同年代大豆品种根系活力的变化及其与植株生物量的关系[J]. 植物生理学报, 2015, 51(3): 345-353.
[17]	Aaron Santner, Luz Irina A Calderon-Villalobos, Mark Estelle. Plant hormones are versatile chemical regulators of plant growth[J]. Nature Chemical Biology, 2009, 5(5): 301-307.
[18]	Costa CTD, Almeida MRD, Ruedell CM, et al.When stress and development go hand in hand: main hormonal controls of adventitious rooting in cuttings[J]. Frontiers in Plant Science, 2013, 4(7): 1-19.
[19]	姚永宏, 吴全, 李中林, 等. 茶树插穗生根过程中可溶性糖等生化成分的动态变化[J]. 西南大学学报(自然科学版), 2006, 28(3): 510-512.
[20]	李丰, 张丽霞, 王乃栋, 等. 茶树枝梢空中压条后不定根发育过程的初步研究[J]. 茶叶科学, 2011, 31(5): 379-385.

顶梢和功能叶对茶树全光照弥雾扦插快繁的影响研究

Effects of Apical -shoots and Functional Leaves on the Fast Propagation of Tea Cuttings under the Condition of Full-illumination and Mist

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献 20

相关文章 15

Metrics

本文评价

推荐阅读 10

[1]	王留彬, 黄丽蕴, 滕翠琴, 吴立赟, 成浩, 于翠平, 王丽鸳. 梧州茶树种质资源的遗传多样性及亲缘关系分析[J]. 茶叶科学, 2022, 42(5): 601-609.
[2]	周汉琛, 杨霁虹, 徐玉婕, 吴琼, 雷攀登. 香叶醇生物合成相关基因NUDX1的进化分析[J]. 茶叶科学, 2022, 42(5): 638-648.
[3]	陈琪予, 马建强, 陈杰丹, 陈亮. 利用图像特征分析茶树成熟叶表型的遗传多样性[J]. 茶叶科学, 2022, 42(5): 649-660.
[4]	孙悦, 吴俊, 韦朝领, 刘梦月, 高晨曦, 张灵枝, 曹士先, 余顺甜, 金珊, 孙威江. 抗小贯松村叶蝉和茶棍蓟马的茶树种质筛选及其抗性相关因素分析[J]. 茶叶科学, 2022, 42(5): 689-704.
[5]	王玉源, 刘任坚, 刘少群, 舒灿伟, 孙彬妹, 郑鹏. 茶树R2R3-MYB转录因子CsTT2表达分析及功能初步鉴定[J]. 茶叶科学, 2022, 42(4): 463-476.
[6]	刘建军, 张金玉, 彭叶, 刘晓博, 杨云, 黄涛, 温贝贝, 李美凤. 不同光质摊青对夏秋茶树鲜叶挥发性物质及其绿茶品质影响研究[J]. 茶叶科学, 2022, 42(4): 500-514.
[7]	邢安琪, 武子辰, 徐晓寒, 孙怡, 王艮梅, 王玉花. 茶树富集氟的特点及其机制的研究进展[J]. 茶叶科学, 2022, 42(3): 301-315.
[8]	王涛, 王艺清, 漆思雨, 周喆, 陈志丹, 孙威江. 茶树CLH基因家族的鉴定与转录调控研究及其在白化茶树中的表达分析[J]. 茶叶科学, 2022, 42(3): 331-346.
[9]	刘任坚, 王玉源, 刘少群, 舒灿伟, 孙彬妹, 郑鹏. 茶树CsbHLH024和CsbHLH133转录因子功能鉴定[J]. 茶叶科学, 2022, 42(3): 347-357.
[10]	欧阳珂, 张成, 廖雪利, 坤吉瑞, 童华荣. 基于感官组学分析玉米香型南川大茶树工夫红茶特征香气[J]. 茶叶科学, 2022, 42(3): 397-408.
[11]	刘富浩, 范延艮, 王域, 孟凡月, 张丽霞. 茶树黄金芽CsHIPP26.1蛋白螯合离子的筛选与鉴定[J]. 茶叶科学, 2022, 42(2): 179-186.
[12]	杨妮, 李逸民, 李静文, 滕瑞敏, 陈益, 王雅慧, 庄静. 外源5-ALA对干旱胁迫下茶树叶绿素合成和荧光特性及关键酶基因表达的影响[J]. 茶叶科学, 2022, 42(2): 187-199.
[13]	疏再发, 郑生宏, 邵静娜, 周慧娟, 吉庆勇, 刘瑜, 何卫中, 王丽鸳. 不同茶树品种（系）对减半施肥的响应研究[J]. 茶叶科学, 2022, 42(2): 277-289.
[14]	陈潇敏, 赵峰, 王淑燕, 邵淑贤, 吴文晞, 林钦, 王鹏杰, 叶乃兴. 福建野生茶树资源嘌呤生物碱构成评价及特异资源筛选[J]. 茶叶科学, 2022, 42(1): 18-28.
[15]	刘庆帅, 璩馥榕, 魏梦园, 钟红, 王熠, 陈亮, 金基强. 基于UPLC技术解析金萱×紫娟F₁分离群体代谢物的遗传变异[J]. 茶叶科学, 2022, 42(1): 29-40.