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茶叶科学 ›› 2019, Vol. 39 ›› Issue (3): 335-341.doi: 10.13305/j.cnki.jts.2019.03.011

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外源一氧化氮对低温胁迫下茶树幼苗生理特性的影响

王英姿1,2,李银花1,2,陈金华1,2,刘仲华1,2,黄建安1,2*   

  1. 1. 湖南农业大学教育部茶学重点实验室,湖南 长沙 410128;2. 国家植物功能成分利用工程技术研究中心,湖南 长沙 410128
  • 收稿日期:2018-09-29 修回日期:2018-12-02 出版日期:2019-06-15 发布日期:2019-06-15
  • 作者简介:王英姿,女,实验师,主要从事茶树品质形成及调控方面的研究,83075688@qq.com。*通信作者:jian7513@sina.com
  • 基金资助:
    湖南省教育厅科学研究项目(17C0767)、校科学基金项目(16QN09)

Effects of Exogenous Nitric Oxide on Physiological Characteristics of Tea Plants Under Cold Stress

WANG Yingzi1,2, LI Yinhua1,2, CHEN Jinhua1,2, LIU Zhonghua1,2, HUANG Jian'an1,2*   

  1. 1. Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China; 2. National Research Center of Engineering & Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha 410128, China
  • Received:2018-09-29 Revised:2018-12-02 Online:2019-06-15 Published:2019-06-15

摘要: 为探究外源一氧化氮(Nitric oxide,NO)供体硝普钠(Sodium nitroprusside,SNP)对低温胁迫下茶树幼苗的缓解作用,采用人工气候室模拟低温胁迫试验,研究了叶面喷施不同浓度SNP对低温胁迫下两年生碧香早茶苗叶片膜脂过氧化程度、渗透调节物质含量及抗氧化酶活性的影响。结果表明,适宜SNP浓度可降低低温胁迫下茶树叶片相对电导率,抑制丙二醛含量的升高,促进脯氨酸、可溶性蛋白和可溶性糖的大量积累,提高超氧化物歧化酶、过氧化氢酶活性,从而缓解低温胁迫对茶树叶片的伤害。试验结果表明,喷施浓度为200 μmol?L-1的SNP效果最佳。

关键词: 茶树, 低温胁迫, 外源NO, 硝普钠, 生理特性

Abstract: In order to investigate the alleviation effect of exogenous nitric oxide (NO) donor sodium nitroprusside (SNP) on tea rooted cuttings under cold stress, artificial climate chamber was used to simulate cold stress on tea rooted cuttings. The effects of SNP on membrane lipid peroxidation, osmotic adjustment substance and antioxidant activity of biennial ‘Bixiangzao’ rooted cuttings under cold stress were studied by foliar spray of SNP. The results show that suitable SNP concentration treatment could reduce the relative conductivity of tea leaves, inhibit the elevated malondialdehyde content, promote the accumulation of proline, soluble protein and sugar, and increase the superoxide dismutase, catalase activities, thereby alleviate the damage of cold stress on tea leaves. In addition, the optimal concentration of SNP was 200 μmol?L-1.

Key words: tea plant, low temperature stress, exogenous nitric oxide, sodium nitroprusside, physiological characteristics

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