白茶萎凋过程萜烯类合成相关基因的鉴定和表达分析

陈雪津; 王鹏杰; 林馨颖; 谷梦雅; 郑玉成; 郑知临; 叶乃兴

doi:10.13305/j.cnki.jts.2020.03.007

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茶叶科学 ›› 2020, Vol. 40 ›› Issue (3) : 363-374. DOI: 10.13305/j.cnki.jts.2020.03.007

白茶萎凋过程萜烯类合成相关基因的鉴定和表达分析

陈雪津, 王鹏杰, 林馨颖, 谷梦雅, 郑玉成, 郑知临, 叶乃兴^*

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Identification and Expression Analysis of Terpene Synthesis Related Genes during the Withering of White Tea

CHEN Xuejin, WANG Pengjie, LIN Xinying, GU Mengya, ZHENG Yucheng, ZHENG Zhilin, YE Naixing^*

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摘要

萜烯类化合物是植物中重要的次生代谢产物之一,对茶树挥发性香气的组成起着重要作用。从茶树基因组数据库中鉴定获得了141个茶树萜烯类合成相关基因,并对其不同组织表达特异性进行分析,筛选出16个在茶树顶芽和嫩叶中高表达的萜烯类合成代表基因。生物信息学分析结果表明,系统进化关系将茶树与拟南芥和葡萄的萜烯类合成相关基因分成了4个亚家族;茶树萜烯类合成相关基因含有5~14个外显子,在上游启动子区域分析发现了大量与光响应、植物生长发育、激素和胁迫响应密切相关的顺式作用元件。荧光定量检测发现,CsMVK、CsDXS和CsGGPS在白茶萎凋过程中的表达显著上调;CsDXR、CsMCT、CsCMK、CsMCS、CsHDS、CsGPPS和CsGGPPS在萎凋4 h和24 h的表达达到峰值。本研究结果为进一步挖掘茶叶萎凋过程中萜烯类合成相关基因对茶叶香气组分积累提供了理论依据。

Abstract

Terpenes are the important secondary metabolites in plants and play an important role in the composition of the volatile aroma of tea plants. In this study, 141 tea plant terpenoid synthesis-related genes were identified from the tea plant genome database. Their expression specificities in different tissues were analyzed. Sixteen terpene synthetic genes which were highly expressed in the apical buds and young leaves of tea plants were screened. The results of bioinformatics methods show that the phylogenetic relationship divides the genes related to terpene synthesis of tea plant, Arabidopsis and grape into four subfamilies. The terpenoid synthesis related genes contain 5 to 14 exons and a large number of cis-related elements closely related to light response, plant growth and development, hormone and stress response according to the upstream promoter region analysis. Fluorescence quantitative detection showed that the expressions of CsMVK, CsDXS and CsGGPS were significantly up-regulated during the withering process of white tea. The expressions of CsDXR, CsMCT, CsCMK, CsMCS, CsHDS, CsGPPS and CsGGPPS showed the highest expressions at 4 h and 24 h after withering. The results of this study provided a theoretical basis for further exploring the functions of terpenoid synthesis related genes in tea.

导出引用

陈雪津, 王鹏杰, 林馨颖, 谷梦雅, 郑玉成, 郑知临, 叶乃兴. 白茶萎凋过程萜烯类合成相关基因的鉴定和表达分析[J]. 茶叶科学. 2020, 40(3): 363-374 https://doi.org/10.13305/j.cnki.jts.2020.03.007

CHEN Xuejin, WANG Pengjie, LIN Xinying, GU Mengya, ZHENG Yucheng, ZHENG Zhilin, YE Naixing. Identification and Expression Analysis of Terpene Synthesis Related Genes during the Withering of White Tea[J]. Journal of Tea Science. 2020, 40(3): 363-374 https://doi.org/10.13305/j.cnki.jts.2020.03.007

中图分类号： S571.1 Q946.8

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