金花白茶对高脂饮食小鼠的减肥作用

doi:10.13305/j.cnki.jts.2024.02.014

摘要/Abstract

摘要： 为探究金花白茶对高脂饮食小鼠的减肥作用,选用金花白茶为材料,以未发花白茶为对照,进行茶叶主要理化成分含量检测,并建立小鼠肥胖模型,给予400 mg·kg^-1金花白茶及白茶水提取物灌胃干预,定期记录小鼠体重,进行肥胖相关指标的测定及组织病理学分析。结果显示,白茶发花后黄酮和茶褐素含量明显上升,茶多酚、游离氨基酸、可溶性糖、茶黄素及茶红素含量显著下降,儿茶素组分中仅表儿茶素含量出现显著的上升。金花白茶能有效抑制高脂饮食小鼠的体质量增长,降低其肝脏和白色脂肪指数,减轻炎症及氧化应激,保护肝脏及肠道的结构完整性及功能。与未发花白茶相比,金花白茶在降低小鼠血脂含量及炎症因子水平上效果更佳,推测是由于冠突散囊菌的大量繁殖及黄酮、表儿茶素、茶褐素等物质含量上升所致;而在缓解肝脏氧化应激方面效果不如白茶,推测是发花后表没食子儿茶素没食子酸酯含量大量减少所致。试验结果表明,金花白茶对高脂饮食诱导的肥胖模型小鼠有明显的减肥效果,发花工艺可在一定程度上提升白茶的降脂减肥功效。

关键词: 金花白茶, 白茶, 发花工艺, 降脂减肥

Abstract: The aim of this study was to investigate the effect of Jinhua white tea (JWT) on weight loss of high fat diet mice. In this study, JWT was selected as the material and compared with the white tea without fungal fermentation method. The determination of major chemical components in the tea was conducted. An obesity mouse model was established, and the mice were administered orally with 400 mg·kg^-1 of JWT water extract as an intervention. The body weight of the mice was regularly recorded, and obesity-related indicators were measured, followed by histopathological analysis of the tissues. The results indicate that after fungal fermentation, the contents of flavonoids and theabrownin increased significantly, while the contents of tea polyphenols, free amino acids, soluble sugars, theaflavins and thearubigins decreased significantly. Meanwhile, among the catechin components, only epicatechin content increased significantly. JWT effectively inhibited the body weight gain in high fat diet mice, reduced their liver and white adipose tissue indices, alleviated inflammation and oxidative stress, and protected the structural integrity and function of the liver and intestines. Compared to white tea without fungal fermentation method, JWT significantly reduced the levels of TC, TG and inflammatory factors in mice, which were speculated to be related to the mass reproduction of Eurotium cristatum and the increase of flavonoids, epicatechin, theabrownin and other substances. While white tea shows a more pronounced effect on relieving liver oxidative stress, which was speculated to be due to the significant decrease in the content of epigallocatechin gallate after fungal fermentation. These findings suggest that JWT has a significant weight loss effect on mice with diet-induced obesity, while fungal fermentation method can improve the lipid-lowering and weight-reducing effects of white tea to a certain extent.

Key words: Jinhua white tea, white tea, fungal fermentation method, anti-obesity

中图分类号:

刘振云, 柯菀萍, 周汐芮, 李孟华, 薄佳慧, 叶兴妹, 刘仲华, 肖力争, 林勇. 金花白茶对高脂饮食小鼠的减肥作用[J]. 茶叶科学, 2024, 44(2): 350-362. doi: 10.13305/j.cnki.jts.2024.02.014.

LIU Zhenyun, KE Wanping, ZHOU Xirui, LI Menghua, BO Jiahui, YE Xingmei, LIU Zhonghua, XIAO Lizheng, LIN Yong. Effect of Jinhua White Tea on Weight Loss of High Fat Diet Mice[J]. Journal of Tea Science, 2024, 44(2): 350-362. doi: 10.13305/j.cnki.jts.2024.02.014.

参考文献

[1] Wang L M, Zhou B, Zhao Z P, et al.Body-mass index and obesity in urban and rural China: findings from consecutive nationally representative surveys during 2004-18[J]. Lancet, 2021, 398(10294): 53-63.
[2] Savini I, Catani M, Evangelista D, et al.Obesity-associated oxidative stress: strategies finalized to improve redox state[J]. International Journal of Molecular Sciences, 2013, 14(5): 10497-10538.
[3] Coutinho A K, Glancey G R. Orlistat, an under-recognised cause of progressive renal impairment [J]. Nephrology Dialysis Transplantation, 2013, 28(s4): iv172-iv174.
[4] Liu D M, Wang S Y, Liu Y Q, et al.Fuzhuan brick tea ameliorates hepatic steatosis and steatohepatitis through gut microbiota-derived aryl hydrocarbon receptor ligands in high-fat diet-induced obese mice[J]. Food & Function, 2023, 14(18): 8351-8368.
[5] Ouyang J, Li X P, Liu C W, et al.Moringa-Fu brick tea extract attenuated high-fat diet-induced obesity via modulating bile acid metabolism and gut microbiota in rats[J]. Journal of Functional Foods, 2023, 109: 105766. doi: 10.1016/j.jff.2023.105766.
[6] Camargo L E A, Pedroso L S, Vendrame S C, et al. Antioxidant and antifungal activities of Camellia sinensis (L.) Kuntze leaves obtained by different forms of production[J]. Brazilian Journal of Biology, 2016, 76: 428-434.
[7] Dias T R, Alves M G, Rato L, et al.White tea intake prevents prediabetes-induced metabolic dysfunctions in testis and epididymis preserving sperm quality[J]. Journal of Nutritional Biochemistry, 2016, 37: 83-93.
[8] Zhou F, Zhu M Z, Tang J Y, et al.Six types of tea extracts attenuated high-fat diet-induced metabolic syndrome via modulating gut microbiota in rats[J]. Food Research International, 2022, 161: 111788. doi: 10.1016/j.foodres.
2022.111788.
[9] 李俊杰, 徐元昊, 陈梦娟, 等. 手筑茯砖茶加工过程中挥发性组分变化分析[J]. 食品科学, 2020, 41(24): 144-154.
Li J J, Xu Y H, Chen M J, et al.Analysis of changes in volatile components during processing of handmade Fuzhuan tea[J]. Food Science, 2020, 41(24): 144-154.
[10] Kang D D, Su M, Duan Y W, et al.Eurotium cristatum, a potential probiotic fungus from Fuzhuan brick tea, alleviated obesity in mice by modulating gut microbiota[J]. Food & Function, 2019, 10(8): 5032-5045.
[11] 薄佳慧, 宫连瑾, 叶兴妹, 等. 金花白茶加工过程中主要滋味物质的动态变化[J]. 现代食品科技, 2022, 38(1): 306-314, 20.
Bo J H, Gong L J, Ye X M, et al.Dynamic changes of main quality components in Jinhua white tea processing[J]. Modern Food Science and Technology, 2022, 38(1): 306-314, 20.
[12] Wang Z H, Liang Y L, Gao C X, et al.The flavor characteristics and antioxidant capability of aged Jinhua white tea and the mechanisms of its dynamic evolution during long-term aging[J]. Food Chemistry, 2024, 436: 137705. doi: 10.1016/j.foodchem.2023.137705.
[13] 缪伊雯, 周静芸, 杨春明, 等. 不同茶树品种寿眉白茶品质分析[J/OL]. 食品工业科技, 2023: 1-18. [2024-02-13]. https://doi.org/10.13386/j.issn1002-0306.2023080168.
Miao Y W, Zhou J Y, Yang C M, et al. Analysis of quality in shoumei white tea from different tea plant varieties [J/OL]. Science and Technology of Food Industry, 2023: 1-18. [2024-02-13]. https://doi.org/10.13386/j.issn1002-0306.2023080168.
[14] 陈阳. 基于肠道菌群的葛根芩连汤干预急性肠炎模型大鼠的作用机制研究[D]. 上海: 上海中医药大学, 2019.
Chen Y.Study on the mechanism of Gegen Qinlian decoction based on gut microbiota in model rats with acute enteritis [D]. Shanghai: Shanghai University of Traditional Chinese Medicine, 2019.
[15] Shi J, Yang G Z, You Q S, et al.Updates on the chemistry, processing characteristics, and utilization of tea flavonoids in last two decades (2001-2021)[J]. Critical reviews in Food Science and Nutrition, 2023, 63(20): 4757-4784.
[16] 蒋陈凯. 普洱生茶“发花”前后的生化成分及降脂活性研究[D]. 广州: 华南农业大学, 2017.
Jiang C K.Biochemical components of Pu’er raw tea before and after flowering and their effects on lipid-lowering [D]. Guangzhou: South China Agricultural University, 2017.
[17] Yang Q Y, Xiao T C, Guo J, et al.Complex relationship between obesity and the fat mass and obesity locus[J]. International Journal of Biological Sciences, 2017, 13(5): 615-629.
[18] 王蝶, 黄建安, 叶小燕, 等. 茯砖茶减肥作用研究[J]. 茶叶科学, 2012, 32(1): 81-86.
Wang D, Huang J A, Ye X Y, et al.The anti-obesity effects of Fuzhuan brick tea on high-fat-diet induced obesity in rats[J]. Journal of Tea Science, 2012, 32(1): 81-86.
[19] Sousa J N, Paraíso A F, Andrade J M, et al.Oral gallic acid improve liver steatosis and metabolism modulating hepatic lipogenic markers in obese mice[J]. Experimental Gerontology, 2020, 134: 110881. doi: 10.1016/j.exger.2020.
110881.
[20] Ardissino M, Vincent M, Hines O, et al.Long-term cardiovascular outcomes after orlistat therapy in patients with obesity: a nationwide, propensity-score matched cohort study[J]. European Heart Journal Cardiovascular Pharmacotherapy, 2022, 8(2): 179-186.
[21] 吕鑫, 肖颖, 李荧, 等. 苦丁茶对高胆碱饮食小鼠肝、肾及氧化应激的影响[J]. 食品研究与开发, 2022, 43(2): 51-57.
Lü X, Xiao Y, Li Y, et al.Effects of Kuding cha on liver, kidney, and oxidative stress in mice fed a high choline diet[J]. Food Research and Development, 2022, 43(2): 51-57.
[22] Singh S, Dulai P, Zarrinpar A, et al.Obesity in IBD: epidemiology, pathogenesis, disease course and treatment outcomes[J]. Nature Reviews Gastroenterology & Hepatology, 2017, 14(2): 110-121.
[23] Xu J L, Li M X, Zhang Y, et al.Huangjinya black tea alleviates obesity and insulin resistance via modulating fecal metabolome in high-fat diet-fed mice[J]. Molecular Nutrition & Food Research, 2020, 64(22): 2000353. doi: 10.1002/mnfr.202000353.
[24] Xu X Y, Zhao C N, Li B Y, et al.Effects and mechanisms of tea on obesity[J]. Critical Reviews in Food Science and Nutrition, 2023, 63(19): 3716-3733.
[25] 蔡正安, 刘素纯, 刘杏益, 等. 冠突散囊菌在不同茶类及几种植物材料上“发花”的研究[J]. 茶叶科学, 2010, 30(4): 263-268.
Cai Z A, Liu S C, Liu X Y, et al.Study of the growth of Eurotium cristatum fungi on some kinds of tea and plant materials[J]. Journal of Tea Science, 2010, 30(4): 263-268.
[26] 刘菲, 薛志慧, 叶倩林, 等. “发花”对白茶风味品质的影响研究[J]. 茶叶科学, 2016, 36(3): 301-311.
Liu F, Xue Z H, Ye Q L, et al.Study on effect of fungal-fermentation process on flavor quality in white tea[J]. Journal of Tea Science, 2016, 36(3): 301-311.
[27] 周遵明, 谭梅傲, 彭冲, 等. 黄芪葛根汤对高脂血症大鼠脂质代谢和氧化应激的影响[J]. 中国中西医结合杂志, 2022, 42(2): 195-201.
Zhou Z M, Tan M A, Peng C, et al.Impact of Huangqi Gegen formula on lipid metabolism and oxidative stress in hyperlipidemia rats[J]. Chinese Journal of Integrated Traditional and Western Medicine, 2022, 42(2): 195-201.
[28] Ma H, Zhang B W, Hu Y Z, et al.Correlation analysis of intestinal redox state with the gut microbiota reveals the positive intervention of tea polyphenols on hyperlipidemia in high fat diet fed mice[J]. Journal of Agricultural and Food Chemistry, 2019, 67: 7325-7335.
[29] Li Q, Liu Z H, Huang J A, et al.Anti-obesity and hypolipidemic effects of Fuzhuan brick tea water extract in high-fat diet-induced obese rats[J]. Journal of the Science of Food & Agriculture, 2013, 93(6): 1310-1316.
[30] Zhang B, Ren D Y, Zhao A Q, et al.Eurotium cristatum reduces obesity by alleviating gut microbiota dysbiosis and modulating lipid and energy metabolism[J]. Journal of the Science of Food and Agriculture, 2022, 102(15): 7039-7051.
[31] Zhen Q C, Liang Q J, Wang H C, et al.Theabrownin ameliorates liver inflammation, oxidative stress, and fibrosis in MCD diet-fed C57BL/6J mice[J]. Frontiers in Endocrinology, 2023, 14: 1118925. doi: 10.3389/fendo. 2023.
1118925.
[32] 钟振威, 卢青, 丁世芳. 茶褐素对高脂喂养ApoE-/-小鼠主动脉粥样硬化作用及机制的研究[J]. 华南国防医学杂志, 2020, 34(3): 151-155.
Zhong Z W, Lu Q, Ding S F.Study the effects and mechanism of theabrownin on aortic atherosclerosis in ApoE-/-mice fed with high-fat diet[J]. Military Medicine of Joint Logistics, 2020, 34(3): 151-155.
[33] Sudhakaran M, Doseff A.The targeted impact of flavones on obesity-induced inflammation and the potential synergistic role in cancer and the gut microbiota[J]. Molecules, 2020, 25(11): 2477. doi: 10.3390/molecules25112477.
[34] Qu Z H, Liu A L, Li P H, et al.Advances in physiological functions and mechanisms of (-)-epicatechin[J]. Critical Reviews in Food Science and Nutrition, 2021, 61(2): 211-233.
[35] 陈敏, 周金凤, 韦崇万, 等. 白藜芦醇和EGCG联用对小鼠骨骼肌抗氧化能力的影响[J]. 南方农业学报, 2022, 53(9): 2683-2690.
Chen M, Zhou J F, Wei C W, et al.Effects of resveratrol and EGCG combination on antioxidant capacity of mouse skeletal muscle[J]. Journal of Southern Agriculture, 2022, 53(9): 2683-2690.