茶树LOX基因家族的鉴定及其在白茶萎凋过程的表达分析

doi:10.13305/j.cnki.jts.2021.04.004

摘要/Abstract

摘要： 脂肪族类化合物是植物芳香物质的重要组成部分,对白茶香气的形成具有重要作用。利用生物信息学方法,在染色体级别的茶树基因组数据库中,对LOX基因家族进行鉴定,从中获得12个茶树LOX基因家族成员,命名为CsLOX1~CsLOX12。12个茶树LOX基因序列,主要定位于细胞质或叶绿体中,其编码蛋白具有相同的特征结构域及保守基序。系统进化树分析表明LOX基因家族分为9-LOX和13-LOX两个亚家族,CsLOX2、CsLOX3、CsLOX4、CsLOX7为9-LOX亚型,其余为13-LOX亚型;基因结构分析表明CsLOX1含有8个外显子,其余均含有9个外显子;不同组织转录组数据分析结果表明,该家族在茶树嫩叶、成熟叶部位高表达;上游启动子区域分析发现大量与植物生长发育、光响应、激素及胁迫响应密切相关的顺式作用元件。荧光定量PCR检测发现,CsLOX基因家族在干旱、低温及茉莉酸甲酯处理下均有不同程度的表达,在白茶不同萎凋时间处理下,CsLOX1、CsLOX3、CsLOX5、CsLOX7、CsLOX8、CsLOX9、CsLOX11和CsLOX12的表达量被诱导上调,4 h时表达量最高（最高上调27倍）。结果表明,CsLOX基因家族成员参与白茶加工萎凋过程脂肪族香气形成的调控,为探明白茶加工过程香气形成的分子机制奠定基础。

关键词: 茶树, LOX基因, 白茶萎凋, 表达分析

Abstract: Aliphatic compounds are an important part of plant aromatic substances and play an important role in the composition of white tea aroma. This study used bioinformatics methods to identify the LOX gene family in the chromosome-level tea plant genome database, and obtained 12 tea plant LOX gene family members, named CsLOX1-CsLOX12. The 12 tea plant LOXs are mainly located in the cytoplasm or chloroplast. The encoded proteins have the same characteristic domains and conserved motifs. Phylogenetic tree analysis shows that the LOX gene family is divided into two subfamilies: 9-LOX and 13-LOX. CsLOX2, CsLOX3, CsLOX4, and CsLOX7 belong to 9-LOX subtypes, and the rest belong to 13-LOX subtypes. Gene structure analysis shows that CsLOX1 contains 8 exons, the rest contain 9 exons. The transcriptome data analysis of different tissues shows that the family genes are highly expressed in the tender and mature leaves of tea plants. The upstream promoter region analysis finds a large number of cis-acting elements closely related to plant development, light response, hormone and stress response. Fluorescence quantitative PCR detection reveals that the CsLOX genes were expressed to varying degrees under drought, low temperature and MeJA hormone treatment. Under the treatment of different withering time of white tea, the expression levels of CsLOX1, CsLOX3, CsLOX5, CsLOX7, CsLOX8, CsLOX9, CsLOX11 and CsLOX12 were induced, with the peaks at 4 h (up to 27-fold increase). The results of this study show that members of the CsLOX gene family participate in the regulation of the formation of aliphatic aromas during the process of white tea withering, laying a foundation for understanding the molecular mechanism of aroma formation during tea processing.

Key words: Camellia sinensis, LOX gene, white tea withering, expression analysis

中图分类号:

S571.1
Q52

林馨颖, 王鹏杰, 陈雪津, 郭永春, 谷梦雅, 郑玉成, 叶乃兴. 茶树LOX基因家族的鉴定及其在白茶萎凋过程的表达分析[J]. 茶叶科学, 2021, 41(4): 482-496. doi: 10.13305/j.cnki.jts.2021.04.004.

LIN Xinying, WANG Pengjie, CHEN Xuejin, GUO Yongchun, GU Mengya, ZHENG Yucheng, YE Naixing. Identification of LOX Gene Family in Camellia sinensis and Expression Analysis in the Process of White Tea Withering[J]. Journal of Tea Science, 2021, 41(4): 482-496. doi: 10.13305/j.cnki.jts.2021.04.004.

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