茶树CsNCED2启动子互作转录因子筛选及在非生物胁迫中的响应

doi:10.13305/j.cnki.jts.2023.03.007

摘要/Abstract

摘要： 9-顺式-环氧类胡萝卜素双加氧酶（NCED）是脱落酸（ABA）合成过程中的关键限速酶,广泛参与植物的生长发育及非生物胁迫响应过程。茶树中CsNCED2参与响应盐胁迫和干旱胁迫,但其转录调控机制尚不明确。通过酵母单杂交文库筛选技术,获得2个结合茶树CsNCED2启动子的转录因子CsDof5.4和CsERF38,亚细胞定位、酵母自激活检测及荧光素酶试验表明,它们都定位于细胞核并能激活CsNCED2的表达。RT-qPCR结果表明,在盐胁迫下,CsERF38和CsDof5.4的表达均与CsNCED2的表达呈显著正相关;干旱胁迫下,只有CsERF38与CsNCED2的表达呈正相关。本研究筛选出2个与茶树CsNCED2启动子互作的转录因子CsDof5.4和CsERF38,而干旱、盐胁迫均能诱导CsNCED2基因上调表达,从而参与茶树非生物胁迫响应过程。

关键词: 茶树, CsNCED2, 转录调控, 表达分析

Abstract: Nine cis epoxide carotenoid dioxygenase (NCED) is a key rate-limiting enzyme in abscisic acid (ABA) synthesis and widely involved in plant growth and development as well as abiotic stress response. CsNCED2 is involved in the response to drought and salt stress in tea plants, while the transcriptional regulation mechanism involved is still unclear. In this study, two transcription factors, CsDof5.4 and CsERF38, which binded to the CsNCED2 promoter were identified by yeast single hybrid (Y1H) library screening. Subcellular localization, yeast self-activation and luciferase (LUC) assay show that they were located in the cell nucleus and could activate the expression of CsNCED2. RT-qPCR results show that the expressions of CsERF38 and CsDof5.4 were highly correlated with CsNCED2 under salt stress. While under drought stress, only the expression of CsERF38was highly correlated with that of CsNCED2. In this study, two transcription factors (CsDof5.4 and CsERF38) binding to the CsNCED2 promoter were identified. Both drought and salt stresses could induce the expression of CsNCED2, thus participate in the abiotic stress response in tea plants.

Key words: tea plant, CsNCED2, transcriptional regulation, expression analysis

中图分类号:

S571.1
Q52

李佳思, 刘迎庆, 张永恒, 张迎澳, 肖烨子, 刘露, 余有本. 茶树CsNCED2启动子互作转录因子筛选及在非生物胁迫中的响应[J]. 茶叶科学, 2023, 43(3): 325-334. doi: 10.13305/j.cnki.jts.2023.03.007.

LI Jiasi, LIU Yingqing, ZHANG Yongheng, ZHANG Ying'ao, XIAO Yezi, LIU Lu, YU Youben. Identification of Transcription Factors Interacting with CsNCED2 Promoter and Their Response to Abiotic Stress[J]. Journal of Tea Science, 2023, 43(3): 325-334. doi: 10.13305/j.cnki.jts.2023.03.007.

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