茶毛虫NPV的P24、Rr1、Lef1基因及其分子进化分析

doi:10.13305/j.cnki.jts.2005.04.002

摘要/Abstract

摘要： 克隆和测定了茶毛虫核型多角体病毒（EupsNPV）全长5942 bp的EcoRI-XhoI基因组DNA片段。序列分析表明该片段包含5个完整的开放阅读框（ORF），分别编码P24、Rr1、38.7 K、Lef1和Ep-ld124。通过对上述5个基因与其它已知杆状病毒的同源基因的分析比较，以及对P24、Rr1和Lef1的分子进化树分析，表明EupsNPV是一种与已知杆状病毒都有较大差异的病毒种类，属于NPV II组病毒。结果还表明EupsNPV与茶尺蠖（Ectropis obliqua）核多角体病毒（EcobSNPV）的亲缘关系最为接近。本文报道的序列的EMBL登录号为AJ920288。

关键词: 茶毛虫, 核型多角体病毒, p24, lef1, Rr1, 序列分析, 分子进化

Abstract: An EcoRI-XhoI 5942 bp DNA fragment from the Euproctis pseudoconspersa nucleopolyhedrovirus (EupsNPV) genome was cloned and its sequence was determined. Five Open Reading Frames (ORFs) encoding P24, Rr1, 38.7 K, Lef1 and Ep-ld124 were identified in the fragment. Comparative and phylogenetic analysis of the 5 genes with their homologues from other baculoviruses revealed that EupsNPV was a nucleopolyhedrovirus distinct from other known baculoviruses. The result also showed that EupsNPV was one of the NPV II members, and was most closely related to Ectropis obliqua SNPV (EcobSNPV). The sequence data reported in this paper have been assigned the EMBL accession number AJ920288.

Key words: Euproctis pseudoconspersa Strand, Nucleopolyhedrovirus, P24, Lef1, Rr1, Phylogenetic tree

中图分类号:

TS272.5+9

聂婷婷, 肖强, 殷坤山, 邢丽苹, 张传溪. 茶毛虫NPV的P24、Rr1、Lef1基因及其分子进化分析[J]. 茶叶科学, 2005, 25(4): 242-248. doi: 10.13305/j.cnki.jts.2005.04.002.

Nie Ting-ting, XIAO Qiang, YIN Kun-shan, XING Li-Ping, ZHANG Chuan-Xi. Phylogenetic Relationship of Tea Tussork(Euproctis pseudoconspersa) Nucleopolyhedrovirus and Other Baculoviruses Based on P24、Rr1 and Lef1 Genes[J]. Journal of Tea Science, 2005, 25(4): 242-248. doi: 10.13305/j.cnki.jts.2005.04.002.

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