本实验选取龙井43幼苗作为研究材料,采用溶液培养的方法,探究不同浓度铝条件下茶树生理变化。结果表明,当铝浓度低于0.4βmmol·L-1时,随铝浓度升高,茶树生长迅速,生物量明显增加,大量新根发生,茶树生长速率与生长量明显高于对照组,且电子传递效率（ETR）随铝浓度升高而增加,根系丙二醛（MDA）含量降低。当铝浓度继续增加时,ETR增长开始下降,生长速率趋于平缓,但仍有大量新根发生,当浓度高于1βmmol·L-1后,Fv/Fm、ETR明显下降,茶树生长速率降低,生长受到抑制。同时,对不同组织进行铝含量测定发现,茶树中铝的分布为侧根、成叶>茎>主根>幼叶,且浓度越高,铝更多的固定在侧根中。研究发现铝浓度低于1.0βmmol·L-1促进了茶树生长,无铝条件与铝浓度高于1βmmol·L-1则不利于茶树幼苗的生长,这些结果为进一步研究铝促进茶树生长的生理机制提供了参考依据。
Abstract
This study was to investigate the physiological response of tea cultivar Longjing 43 seedlings to different aluminum (Al) concentrations by hydroponic culture. The results revealed the biomass increment, relative growth rate (RGR) and electron transfer efficiency (ETR) of the tea plants under low Al concentrations (0.2 and 0.4βmmol·L-1) were significantly higher than the control group. However, The MDA content in roots showed an opposite trend. As Al concentration continued to increase, the increase rate of ETR began to decline and the growth rate became flat but still a great number of fresh roots were coming up. When Al concentration was higher than 1βmmol·L-1, the efficiency of light energy conversion in PSⅡ(Fv/Fm) and ETR showed dramatic decline. Meanwhile, RGR decreased and the growth of tea plant was also inhibited. Simultaneously, the results of Al contents in the different tissues of tea plants followed lateral roots, mature leaves > stems > main roots > young leaves. More Al was found in the lateral roots with Al concentration increasing. The study suggested that when Al concentration was lower than 1.0βmmol·L-1, it could promote the growth of tea plants, but the growth would be inhibited under an Al-free environment or the Al concentration was higher than 1 mmol·L-1. These results laid a foundation for further study on the physiological mechanism of how Al would promote the growth of tea plants.
关键词
茶树 /
促进生长 /
铝 /
生理响应
Key words
physiology response /
promote growth /
tea plant /
aluminum
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基金
国家自然科学基金（31572199）、中国农业科技创新工程项目（CAAS-ASTIP-2014-TRICAAS-03）
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