茶树生物钟CsLUX基因的鉴定及其对光合特性的影响

茶叶科学 ›› 2025, Vol. 45 ›› Issue (4): 559-570.

茶树生物钟CsLUX基因的鉴定及其对光合特性的影响

孙梦真¹, 胡志航¹, 杨凯欣¹, 张佳琪¹, 张楠², 熊爱生², 刘慧², 庄静^1,*

1.南京农业大学园艺学院茶叶科学研究所,农业农村部华东地区园艺作物生物学与种质创制重点实验室,江苏南京 210095;
2.南京农业大学,作物遗传与种质创新利用全国重点实验室,江苏南京 210095

收稿日期:2025-01-07 修回日期:2025-04-04 出版日期:2025-08-15 发布日期:2025-08-15
通讯作者: *zhuangjing@njau.edu.cn
作者简介:孙梦真,女,硕士研究生,主要从事茶树遗传育种方面的研究。
基金资助:
江苏省政策引导类计划（SZ-LYG202126）、江苏园艺种质资源保护和创新计划（JSFEM-202212）、江苏高校优势学科建设项目（PAPD）

Identification of Circadian Clock CsLUX Gene and Its Effects on Photosynthetic Characteristics in Tea Plants

SUN Mengzhen¹, HU Zhihang¹, YANG Kaixin¹, ZHANG Jiaqi¹, ZHANG Nan², XIONG Aisheng², LIU Hui², ZHUANG Jing^1,*

1. Ministry of Agriculture and Rural Affair Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, Tea Research Institution, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;
2. State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China;

Received:2025-01-07 Revised:2025-04-04 Online:2025-08-15 Published:2025-08-15

摘要/Abstract

摘要： 茶树是我国栽培面积较大的叶用经济作物,其生长发育机制受到生物钟相关基因的严格调控。以‘龙井43’的cDNA为模板克隆CsLUX基因,对其进行序列比对和系统进化分析;在两个光周期（48 h）内,利用RT-qPCR技术检测CsLUX基因的相对表达量,同时测定茶树叶片中光合参数、气孔开度以及叶绿素含量。结果显示,CsLUX基因全长为951 bp,编码由316个氨基酸组成的亲水性蛋白;其二级结构主要由α-螺旋和无规则卷曲组成,且CsLUX与CCA1、ELF3等生物钟关键元件作用较为密切;不同时间点茶树叶片光合参数的变化趋势与气孔开度基本一致,叶绿素SPAD值基本稳定不变;CsLUX的相对表达量在夜间达到峰值,在白天维持较低的表达水平,响应昼夜节律的变化。茶树基因CsLUX在两个光周期内的表达量与光合参数的节律性变化呈现一定的相关性。

关键词: 茶树, 生物钟, CsLUX, 光合特性, 表达分析

Abstract: Tea is a cash crop with large cultivated area in China, and its growth and development mechanism are strictly regulated by genes related to biological clock. In this study, the CsLUX gene was cloned using cDNA from tea cultivar ‘Longjing 43’ as template and sequence alignment and phylogenetic analysis were performed. Within two photoperiodic periods (48 h), the relative expression of CsLUX gene was detected by RT-qPCR, and photosynthetic parameters, stomatal opening and chlorophyll contents in tea leaves were also determined. The total length of CsLUX gene is 951 bp, encoding a hydrophilic protein composed of 316 amino acids. The secondary structure is mainly composed of α-helix and random curling. CsLUX is closely related to CCA1, ELF3 and other key components of the biological clock. The variation trend of photosynthetic parameters in tea leaves at different time points was basically consistent with stomatal opening, and the value of chlorophyll SPAD was basically stable. The relative expression of CsLUX reached its peak under dark conditions and maintained a low expression level during the day in response to changes in circadian rhythm. The expression of CsLUX gene was correlated with the rhythmic changes of photosynthetic parameters in two photoperiodic periods (48 h) in tea plants.

Key words: tea plant, circadian clock, CsLUX, photosynthetic characteristics, expression analysis

中图分类号:

S571.1
TS272

孙梦真, 胡志航, 杨凯欣, 张佳琪, 张楠, 熊爱生, 刘慧, 庄静. 茶树生物钟CsLUX基因的鉴定及其对光合特性的影响[J]. 茶叶科学, 2025, 45(4): 559-570.

SUN Mengzhen, HU Zhihang, YANG Kaixin, ZHANG Jiaqi, ZHANG Nan, XIONG Aisheng, LIU Hui, ZHUANG Jing. Identification of Circadian Clock CsLUX Gene and Its Effects on Photosynthetic Characteristics in Tea Plants[J]. Journal of Tea Science, 2025, 45(4): 559-570.

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