六堡茶对高脂饮食大鼠肠道短链脂肪酸含量的影响

doi:10.13305/j.cnki.jts.2019.02.011

摘要/Abstract

摘要： 探讨六堡茶对高血脂症模型大鼠肠道短链脂肪酸的影响。以低剂量50 mg·kg^-1·d^-1、中剂量100 mg·kg^-1·d^-1和高剂量200 mg·kg^-1·d^-1六堡茶水提物对高脂模型大鼠进行干预性治疗4周后,收集大鼠粪便;将六堡茶醇提物与高脂模型空白组大鼠粪便进行体外发酵培养,最后采用气相色谱法分别测定粪便和发酵液短链脂肪酸含量。体内试验结果表明,六堡茶水提物能显著降低高脂大鼠肠道的乙酸含量,显著增加高脂大鼠肠道的丙酸和丁酸的含量;与绿茶水提物相比,六堡茶对高脂大鼠短链脂肪酸的影响更强。体外试验结果表明,六堡茶醇提物对乙酸的抑制率为16.92%,对丙酸和丁酸的促进率分别为24.81%和23.03%;4种茶叶中,六堡茶对肠道短链脂肪酸的影响最大,普洱熟茶次之,六堡原料毛茶和绿茶的影响最小;六堡茶醇提物不同极性段组分均能抑制乙酸分泌、促进丙酸和丁酸的分泌,其中,六堡茶醇提物通过60%的乙醇洗脱组分对肠道短链脂肪酸的影响最大。

关键词: 六堡茶, 短链脂肪酸（SCFAs）, 高脂大鼠, 肠道

Abstract: The objective of this work was to investigate the effect of Liupao tea on levels of short chain fatty acid (SCFs) in intestinal tract of rats with hyperlipidemia. Firstly, rats with hyperlipidemia were fed with different dosages of Liupao tea water extract (low dosage, 50 mg·kg^-1·d^-1, medium dosage, 100 mg·kg^-1·d^-1 and high dosage, 200 mg·kg^-1·d^-1) for 4 weeks, collected the feces samples. Secondly, the Liupao tea ethanol extracts were cultured with fecal of model blank group in vitro. Finally, SCFAs in feces or fermentation broth were determined by gaschromatography (GC). The results in vivo show that Liupao tea water extract significantly reduced the level of acetate and increased propionate and butyrate in the intestinal tract of rats with hyperlipidemia. Compared with green tea water extract, the effect of Liupao tea water extract on levels of SCFAs in the intestinal tract of rats with hyperlipidemia was stronger. The results in vitro show that the inhibition rate of Liupao tea ethanol extract to acetate, the promotion rates of Liupao tea ethanol extract to propionate and butyrate were 16.92%, 24.81% and 23.03% respectively. Among the 4 types of tea, Liupao tea had the greatest influence on levels of the short-chain fatty acids in the intestine, followed by puer tea, and raw liupao tea and green tea had the smallest impact. Different polar components of Liupao tea alcohol extract can inhibit the production of acetate, promote the productions of propionate and butyrate. However, the effect of 60% alcohol elution component on the production of SCFAs in the intestinal tract of rats was the greatest.

Key words: Liupao tea, short-chain fatty acids (SCFAs), rats, gastrointestinal tract

中图分类号:

S571.1
R285

叶颖, 韦保耀, 滕建文, 夏宁, 黄丽, 曾司斌. 六堡茶对高脂饮食大鼠肠道短链脂肪酸含量的影响[J]. 茶叶科学, 2019, 39(2): 211-219. doi: 10.13305/j.cnki.jts.2019.02.011.

YE Ying, WEI Baoyao, TENG Jianwen, XIA Ning, HUANG Li, ZENG Sibin. Effect of Liupao Tea on Levels of Short Chain Fatty Acids in Intestinal Tract of Rats with Hyperlipidemia[J]. Journal of Tea Science, 2019, 39(2): 211-219. doi: 10.13305/j.cnki.jts.2019.02.011.

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