西双版纳茶区茶轮斑病的致病病原分离及鉴定

茶叶科学

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西双版纳茶区茶轮斑病的致病病原分离及鉴定

朱罗娟^1,2，江冰冰^1,3，马玉青⁴，毕玲芳^1,2，侯国喜^1,2，李瑞^1,2，刘弟^1,2，段斯妍^1,2，张锦^1,2，朱书生^1,2，杨敏^1,2*，郭存武^1,2*

1. 农业生物多样性应用技术国家工程研究中心/云南省生物资源保护与利用国家重点实验室云南农业大学，云南昆明 650201；2. 云南农业大学植物保护学院，云南昆明 650201；3. 云南农业大学茶学院，云南昆明 650201；4. 云南省农业科学院茶叶研究所，云南昆明 650205

出版日期:2025-10-23 发布日期:2025-10-23
通讯作者: 杨敏，yangminscnc@126.com；郭存武，gcw0220@163.com
作者简介:朱罗娟，女，硕士生，主要从事植物病理学方面的研究
基金资助:
云南省科学研究基金资助性项目（2024J0406）、云南省基础研究农业联合专项（202401BD070001-054）、云南省兴滇英才青年人才专项（XDYC-QNRC-2023-0404、YDYC-QNRC-2023-0399）

Isolation and Identification of Pathogens Causing Tea Gray Blight in Xishuangbanna Tea Area

ZHU Luojuan^1,2, JIANG Bingbing^1,3, MA Yuqing⁴, BI Lingfang^1,2, HOU Guoxi^1,2, LI Rui^1,2, LIU Di^1,2, DUAN Siyan^1,2, ZHANG Jin^1,2, ZHU Shusheng^1,2, YANG Min^1,2*, GUO Cunwu^1,2*

1. National Engineering Research Center for Applied Technology of Agricultural Biodiversity, State Key Laboratory for Conservation and Utilization of Bio-Resourcesin Yunnan, Yunnan Agricultural University, Kunming 650201, China;2. College of Plant Protection, Yunnan AgriculturalUniversity, Kunming 650201, China; 3. College of Tea Science, Yunnan Agricultural University, Kunming 650201, China; 4. Tea Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China

Online:2025-10-23 Published:2025-10-23
Contact: YANG Min，yangminscnc@126.com；GUO Cunwu，gcw0220@163.com

摘要/Abstract

摘要： 茶轮斑病是茶园中主要的叶部病害之一，严重影响茶叶产量和品质。2023年7月在云南省西双版纳傣族自治州勐腊县茶园发现疑似轮斑病症状的茶树病叶，但鉴于该症状的病原菌在该区域尚未见报道，本研究采用组织分离法、伤口接种法进行致病性测定、形态学观察和基于ITS和TUB基因序列的分子系统发育分析相结合的方法开展了致病菌的鉴定及其物种分类，并成功分离、鉴定了该病原菌。结果表明，从轮斑病症状病叶分离得到的菌株FN3-2具有致病性；根据其形态特征并结合分子系统发育证据，最终将病原菌鉴定为山龙眼新拟盘多毛孢（Neopestalotiopsisprotearum）。研究结果为该区域轮斑病的精准诊断和防控提供科学依据，为茶轮斑病生物防治菌剂的筛选提供了病原学依据，为该地区茶产业的健康可持续发展奠定基础。

关键词: 茶, 轮斑病, 山龙眼新拟盘多毛孢, 病原鉴定

Abstract: Tea gray blight is one of the major foliar diseases in tea plantations, significantly affecting both yield and quality. In July 2023, tea leaves exhibiting resembling gray blight symptoms were observed during a filed surver in tea plantations in Mengla County, Xishuangbanna Dai Autonomous Prefecture, Yunnan Province. As the causal pathogen of these symptoms had not been previously reported in this region, this study aimedto identify the causal agent and its taxonomic classification.The pathogen was successfullyisolated and identified usinga combination of tissue isolation, pathogenicity testing via wound inoculation, morphological characterization, and molecular phylogenetic analysis based on ITS and TUB genessquences. The results confirmed that the strain FN3-2, isolated from symptomatic leaves, was pathogenic. Based on morphological traitsand molecular evidence, the pathogen was finally identified as Neopestalotiopsisprotearum. The finding provided a scientific basis for the accurate disease diagnosis and biocontrol agent screening, contributing to the sustainable development of tea production in the region.

朱罗娟^{1, 2}, 江冰冰^{1, 3}, 马玉青⁴, 毕玲芳^{1, 2} 侯国喜^{1, 2}, 李瑞^{1, 2}, 刘弟^{1, 2}, 段斯妍^{1, 2}, 张锦^{1, 2}, 朱书生^{1, 2}, 杨敏^{1, 2}, 郭存武^{1, 2}. 西双版纳茶区茶轮斑病的致病病原分离及鉴定[J]. 茶叶科学.

ZHU Luojuan^{1, 2}, JIANG Bingbing^{1, 3}, MA Yuqing⁴, BI Lingfang^{1, 2}, HOU Guoxi^{1, 2}, LI Rui^{1, 2}, LIU Di^{1, 2}, DUAN Siyan^{1, 2}, ZHANG Jin^{1, 2}, ZHU Shusheng^{1, 2}, YANG Min^{1, 2}, GUO Cunwu^{1, 2}. Isolation and Identification of Pathogens Causing Tea Gray Blight in Xishuangbanna Tea Area[J]. Journal of Tea Science.

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西双版纳茶区茶轮斑病的致病病原分离及鉴定

Isolation and Identification of Pathogens Causing Tea Gray Blight in Xishuangbanna Tea Area

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