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Journal of Tea Science ›› 2004, Vol. 24 ›› Issue (1): 23-28.doi: 10.13305/j.cnki.jts.2004.01.004

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Study on the Expression of Caffeine Synthase Gene mRNA in Tea Plant

LI Yuan-hua, JIANG Chang-jun※, WAN Xiao-chun   

  1. Key Laboratory of Tea Biochemistry & Biotechnology , Ministry of Agriculture,Anhui Agricultural University,Hefei 230036, China
  • Received:2003-06-23 Online:2004-03-25 Published:2019-09-11

Abstract: The expression of caffeine synthase gene mRNA in tea plant by means of in situ hybridization was investigated. Results showed that the expressional sites is mainly located in the cell cytoplasm and nucleus of palisade tissue, but not in vacuole. The expressional sites of leaf vein are mainly in parenchyma and phloem, however, that in phloem is weaker. The expressional sites in young stalk are in phloem. The expressional sites in different cultivars are basically similar, but the expressional signal is different. Results also showed that the expressional signal of the first fresh leaf in spring is stronger than that in autumn. In terms of processing technique, the green teas which are spread 0.5 h、1 h、2 h showed apparent expressional signal, but the signal was not observed in the black teas withered for 6 h、8 h、10 h and Oolong teas shaken for 6 h、8 h、10 h.

Key words: Tea, Caffeine synthase gene, Expression, MRNA, In situ hybridization

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