Study on Inhibitation of Agrobacterium rhizogenes by Tea Catechin and Screening of Anti-polyphenol Strain

毛清黎，施兆鹏，李玲，刘仲华，朱旗

doi:10.13305/j.cnki.jts.2007.03.006

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Journal of Tea Science ›› 2007, Vol. 27 ›› Issue (3) : 243-247. DOI: 10.13305/j.cnki.jts.2007.03.006

Study on Inhibitation of Agrobacterium rhizogenes by Tea Catechin and Screening of Anti-polyphenol Strain

MAO Qing-li^1,2, SHI Zhao-peng², LI Lin³, LIU Zhong-hua², ZHU Qi²

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Abstract

This paper investigated inhibition of Agrobacterium rhizogenes by tea catechin and screening of the anti-polyphenol strain.The results showed that the catechin (TC80), which MIC90 was 25~100 μg/ml, showed the obvious inhibition of Agrobacterium rhizogenes. This showed that the plenty of polyphenols in the tea plant was a key factor to restrict Agrobacterium rhizogenes-mediated transformation of tea plant. An anti-polyphenol strain R1000AP obtained by anti-polyphenol screening, which anti-polyphenol capability enhanced 10~40 times. The hairy root frequency induced by the strain increased by 46.5%（P<0.05）compared to the non-anti-polyphenol strain.

Key words

anti-polyphenol strain / hairy root frequency / inhibitatory action / screen / Agrobacterium rhizogenes / catechin

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MAO Qing-li, SHI Zhao-peng, LI Lin, LIU Zhong-hua, ZHU Qi. Study on Inhibitation of Agrobacterium rhizogenes by Tea Catechin and Screening of Anti-polyphenol Strain[J]. Journal of Tea Science. 2007, 27(3): 243-247 https://doi.org/10.13305/j.cnki.jts.2007.03.006

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