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Journal of Tea Science ›› 2008, Vol. 28 ›› Issue (6): 459-467.doi: 10.13305/j.cnki.jts.2008.06.006

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Molecular Cloning and Expression Analysis on ACC Oxidase Gene Full-length cDNA from Tea Plant

ZHANG Ya-li, QIAO Xiao-yan, CHEN Liang*   

  1. Research Center for Tea Germplasm and Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Hangzhou 310008, China
  • Received:2008-06-26 Online:2008-12-15 Published:2019-09-12

Abstract: 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase is an important enzyme and plays a critical regulatory role in the biosynthesis of ethylene. Based on previous ESTs sequencing results of the tender shoots cDNA library and RT-PCR technology, a full-length cDNA sequence coding ACC oxidase (ACO) of tea plant was cloned. The GenBank accession is DQ904328 in the NCBI. The ACO gene had 1 232 bp in length, encoding 320 amino acid residues with the putative molecular weight of 36.2 KD and the pI 5.41. The protein sequences of tea plant ACO aligned with those of other 12 plants showed highly conservative sequences in tea plant. Neighbor-Joining phylogenetic tree based on ACO sequence of tea plant and other 22 plants indicated that tea plant had very close relationship with that of Diospyros kaki. The ACO gene expression level of different cultivars after high and low temperature stresses were analyzed using RT-PCR method. The expression level has correlation with resistance of the cultivars to certain degree.

Key words: tea plant (Camellia sinensis), ethylene, ACC oxidase, gene cloning, expression analysis

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