Molecular Cloning and Expression Analysis on ACC Oxidase Gene Full-length cDNA from Tea Plant

ZHANG Ya-li; QIAO Xiao-yan; CHEN Liang

doi:10.13305/j.cnki.jts.2008.06.006

PDF(527 KB)

Journal of Tea Science ›› 2008, Vol. 28 ›› Issue (6) : 459-467. DOI: 10.13305/j.cnki.jts.2008.06.006

Molecular Cloning and Expression Analysis on ACC Oxidase Gene Full-length cDNA from Tea Plant

ZHANG Ya-li, QIAO Xiao-yan, CHEN Liang^*

Author information +

History +

Abstract

1-aminocyclopropane-1-carboxylic acid (ACC) oxidase is an important enzyme and plays a critical regulatory role in the biosynthesis of ethylene. Based on previous ESTs sequencing results of the tender shoots cDNA library and RT-PCR technology, a full-length cDNA sequence coding ACC oxidase (ACO) of tea plant was cloned. The GenBank accession is DQ904328 in the NCBI. The ACO gene had 1 232 bp in length, encoding 320 amino acid residues with the putative molecular weight of 36.2 KD and the pI 5.41. The protein sequences of tea plant ACO aligned with those of other 12 plants showed highly conservative sequences in tea plant. Neighbor-Joining phylogenetic tree based on ACO sequence of tea plant and other 22 plants indicated that tea plant had very close relationship with that of Diospyros kaki. The ACO gene expression level of different cultivars after high and low temperature stresses were analyzed using RT-PCR method. The expression level has correlation with resistance of the cultivars to certain degree.

Key words

ACC oxidase / ethylene / expression analysis / gene cloning / tea plant (Camellia sinensis)

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

ZHANG Ya-li, QIAO Xiao-yan, CHEN Liang. Molecular Cloning and Expression Analysis on ACC Oxidase Gene Full-length cDNA from Tea Plant[J]. Journal of Tea Science. 2008, 28(6): 459-467 https://doi.org/10.13305/j.cnki.jts.2008.06.006

References

[1] 张亚丽, 乔小燕, 陈亮. 茶树ACC合成酶基因的克隆及生物信息学分析[J]. 茶叶科学, 2008, 28(4), 235~241.
[2] Holdsworth MJ, Schuch W, Grierson D.Organization and expression of a wound/ripening-related small multigene family from tomato[J]. Plant Mol Biol, 1988, 11(2): 81~88.
[3] Callahan AM, Morgens PH, Wright P, et al. Comparison of Pch313 (pTOM13 Homolog) RNA accumulation during fruit softening and wounding of two phenotypically different peach cultivars[J]. Plant Physiol, 1992, 100(1): 482~488.
[4] McGarvey DJ, Christoffersen RE. Characterization and kinetic parameters of ethylene-forming enzyme from avocado fruit[J]. J Biol Chem, 1992, 267(9): 5964~5967.
[5] Lasserre E, Bouquin T, Hernandez JA, et al. Structure and expression of three genes encoding ACC oxidase homologs from melon (Cucumis melo L.)[J]. Mol Gen Genet, 1996, 251(1): 81~90.
[6] Wang X, Bauer JH, Li Y, et al. Characterization of a p75 (NTR) apoptotic signaling pathway using a novel cellular model[J]. J Biol Chem, 2001, 276(36): 33812~33820.
[7] 张树珍, 汤火龙, 杨本鹏, 等. 康乃馨ACC氧化酶cDNA的克隆及其反义植物表达载体的构建[J]. 云南植物研究, 2002b, 24(6): 775~780.
[8] 徐晓峰, 黄学林, 黄霞. ACC氧化酶反义基因转化青花菜的研究[J]. 中山大学学报(自然科学版), 2003, 42(4): 64~68.
[9] 胡宗利, 陈国平, 吕丽娟, 等. 环境胁迫和乙烯对番茄ACO1基因表达的影响[J]. 重庆大学学报(自然科学版), 2007, 30(1): 129~133.
[10] 陈亮, 赵丽萍, 高其康. 茶树新梢cDNA文库的构建和ESTs测序成功率初步分析[J]. 茶叶科学, 2004, 24(1): 18~22.
[11] 陈亮, 赵丽萍, 高其康. 茶树新梢cDNA克隆测序和表达序列标签(ESTs)特性分析[J]. 农业生物技术学报, 2005, 13(1): 21~25.
[12] Thompson JD, Higgins DG, Gibson TJ.CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice[J]. Nucleic Acids Res, 1994, 22: 4673~4680.
[13] Kumar S, Tamura K, Nei M.MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment[J]. Briefings in Bioinformatics, 2004, 5: 150~163.
[14] 梅菊芬, 王新超, 杨亚军, 等. 茶树冷驯化过程中基因表达差异的初步分析[J]. 茶叶科学, 2007, 27(4): 286~292.
[15] 张亚丽. 茶树ACS、ACO基因克隆与亲环素基因的鉴定及其表达分析[D]. 杭州: 中国农业科学院硕士学位论文, 2007.
[16] Bouquin T, Lasserre E, Pradier J, et al. Wound and ethylene induction of the ACC oxidase melon gene CM-ACO1 occurs via two direct and independent transduction pathway[J]. Plant Mol Biol, 1997, 35(6):1029~1035.
[17] Yu SJ, Kim S, Lee JS, et al. Differential accumulation of transcripts for ACC synthase and ACC oxidase homologs in etiolated mung bean hypocotyls in response to various stimuli[J]. Molecules and Cells, 1998, 8(3): 350~358.
[18] Nie X, Singh RP, Tai GC.Molecular characterization and expression analysis of 1-aminocyclopropane-1-carboxylate oxidase homologs from potato under abiotic and biotic stresses[J]. Genome, 2002, 45(5): 905~913.
[19] Kim YS, Choi D, Lee MM, et al. Biotic and abiotic stress-related expression of 1-aminocyclopropane-1- carboxylate oxidase gene family in nicotiana glutinosa[J]. Plant Cell Physiol, 1998, 39(6): 565~573.