茶树GDP-D-甘露糖焦磷酸化酶基因cDNA全长的克隆与表达分析

doi:10.13305/j.cnki.jts.2015.01.010

Abstract

Abstract: Ascorbic acid (Vitamin C, Vc) is an important antioxidant in tea plant, playing important roles in metabolism and responses to abiotic stress, and tea quality is positive correlation with the content of Vc. GDP-D-mannose pyrophosphorylase (GMP) is an important enzyme in the synthesis of ascorbic acid. The full-length cDNA sequence of GMP gene was isolated from the shoots of Camellia sinensis by RT-PCR and RACE. The entire GMP cDNA was 1β510βbp, containing a 1β086βbp complete open reading frame which encoding a protein with 361 amino acids and a calculated molecular weight of 39.599βkDa. Blast analysis showed that GMP gene in Camellia sinensis was most closely to Actinidia with 96% amino acids similarity. Quantitative real-time PCR analysis showed that the expression levels of GMP gene in the third leaves were highest while the stem is the lowest, and the different varieties also existed an obvious differences. High temperature stress stimulate the expression of GMP gene and the accumulation of Vc in incipient stage, then reduced rapidly.

Key words: Camellia sinensis, GDP-D-mannose pyrophosphorylase (GMP), gene clonging, expression analysis

CLC Number:

XIAO Yao, ZHOU Tianshan, LI Jiao, ZHANG Jiaxin, YU Youben. Cloning and Expression Analysis of GDP-D-mannose Pyrophosphorylase cDNA in Camellia sinensis[J]. Journal of Tea Science, 2015, 35(1): 55-63.

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Cloning and Expression Analysis of GDP-D-mannose Pyrophosphorylase cDNA in Camellia sinensis

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