Effect of Geographical Translocation on the Chemical Composition and Metabolome of Green Tea from Camellia sinensis cv. Huangshanzhong

WANG Mengqi; SONG Dapeng; YIN Hongxu; ZHOU Chao; FANG Fengxiang; LI Jiyan; YU Jie; ZHANG Na; DING Shibo

doi:10.13305/j.cnki.jts.2025.06.006

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Journal of Tea Science ›› 2025, Vol. 45 ›› Issue (6) : 957-970. DOI: 10.13305/j.cnki.jts.2025.06.006

Effect of Geographical Translocation on the Chemical Composition and Metabolome of Green Tea from Camellia sinensis cv. Huangshanzhong

WANG Mengqi¹, SONG Dapeng¹, YIN Hongxu², ZHOU Chao¹, FANG Fengxiang¹, LI Jiyan¹, YU Jie¹, ZHANG Na³, DING Shibo^1,*

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Abstract

The growing environment significantly influences the accumulation of chemical compounds in tea plants. However, systematic studies on the changes in chemical composition after translocating the tea cultivar ‘Huangshanzhong’ to the high-latitude Shandong region remain scarce. This study investigated the green tea samples of Camellia sinensis cv. Huangshanzhong from two regions: the native origin (YCD, Huangshan, Anhui) and a transplanted region (SD, Rizhao, Shandong). Differences in the content levels of the chemical components were analyzed. Additionally, widely-targeted metabolomics techniques were employed to identify differential metabolites and elucidate core metabolic pathways. The results demonstrate that SD samples exhibited significantly higher levels of water-soluble carbohydrates, total ash, water-soluble ash, water extract content and total galloylated catechins than YCD. While the total polyphenols and caffeine showed no inter-regional difference. SD accumulated more asparagine, glutamate, and arginine, whereas YCD was richer in flavor-related amino acids, such as theanine, GABA, lysine. Metabolomics analysis identified 116 differential metabolites (73 upregulated, 43 downregulated), with flavonoids exhibiting the highest abundance and diversity among them. KEGG enrichment analysis further revealed that the purine metabolism, phenylalanine metabolism, and pyrimidine metabolism pathways were the most significantly enriched pathways, contributing most to the differences between the SD and YCD samples. This study provided a systematic comparison of chemical components in green tea from cv. ‘Huangshanzhong’ after high-latitude transplantation versus the original cultivation, offering scientific insights into tea cultivar adaptation and flavor quality optimization.

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Camellia sinensis cv. Huangshanzhong / chemical components / green tea / high-latitude transplantation / metabolomics

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WANG Mengqi, SONG Dapeng, YIN Hongxu, ZHOU Chao, FANG Fengxiang, LI Jiyan, YU Jie, ZHANG Na, DING Shibo. Effect of Geographical Translocation on the Chemical Composition and Metabolome of Green Tea from Camellia sinensis cv. Huangshanzhong[J]. Journal of Tea Science. 2025, 45(6): 957-970 https://doi.org/10.13305/j.cnki.jts.2025.06.006

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