酶性氧化合成茶黄素条件优化的研究

doi:10.13305/j.cnki.jts.2006.04.010

Abstract

Abstract: Obtain the optimum reaction condition of synthesizing theaflavins by enzymatic oxidation, synthesizing theaflavins was studied by raw ppo extracted from fresh tea leaves under single factor and orthogonal experimental design. Based on these results, synthesizing theaflavins was also studied by using breaking fresh tea leaves. The result showed that the optimum condition of theaflavins synthesizing is 30℃ temperature is, under this reaction system, pH value of the present system is 4.8, the concentration of substrates is 0.9βg/100βml, the concentration of enzymes is 28βg/100βml (based on the fresh weight), oxygen rate is 0.4βL/min. Temperatures and pH values in the reaction system are the most important factors(P<0.05), and adding suitable oxygen is another necessary factor in theaflavins synthesis by enzymatic oxidation.

Key words: theaflavins, catechin, enzymatic oxidation, optimization

CLC Number:

S571.1

GU Ji-ping, LIU Zhong-hua, HUANG Jian-an, SHI Zhao-peng. Study on the Optimum Condition of Synthesizing Theaflavins by Using Enzyme-catalysing Oxidation[J]. Journal of Tea Science, 2006, 26(4): 285-290.

References 8

[1]	Bacus, James V, Kelloff, et al. Chemoprevention with theaflavins of rat esophageal intraepithelial neoplasia quantitatively monitored by image tile analysis.Cancer epidemiology,biomarkers & prevention[M]. A pubication of the American association for cancer research / cosponsored by the American society of preventive oncology, 2000, 9(11): 1149~1154.
[2]	Eng, Qing, Torii, et al. Black tea polyphenols, theaflavins,prevent celluar DNA damige by inhibiting oxidative stress and suppressing cytochrome P450 1A1 in cell cultures[J]. Journal of Agricultrual and food chemistry, 2002, 50(1): 213~220.
[3]	Uptas.Anticlastogenic effects of black tea (World blend) and its tow active polyphenols theaflavins and thearubigins in vivo in Swiss albion mice[J]. Life science, 2001, 69(23): 2735~2744.
[4]	大泽俊彦. 应用植物成分进行化学防癌[J]. 日本医学介绍, 2004, 25(1): 26~27.
[5]	Cong YY, Han C, Chen JS.Effect of tea polyphenols and tea pigments on the inhibition of precancerous 1iver lesions in rats[J]. Nutr And Cancer, 2000, 38(1): 81~86.
[6]	Lin Yu-li, Tsai Shu-huei, Lin-shiau, et a1, Theaflavin-3,3’ -digallate from black tea the nitric oxide synthase by down-regulating the actination of NF-kappa Bin macrophages[J]. Europen J of pharmacology, 1999, 367(2~3): 379~388.
[7]	范远景, 张劲松, 高学云. 茶水提取物和茶多酚抑制诱变的类型及其机制, 茶·癌变·畸变·突变[J]. 2003, 15(3): 153~156,167.
[8]	王坤波, 刘仲华, 黄建安. 儿茶素体外氧化制备茶黄素的研究[J]. 茶叶科学, 2004, 24(1): 53~59.

[1]	JIANG Ating, LIU Qiaofang, XIAO Juanjuan, HE Junhui, GAO Bingcai, HUANG Jian'an, WANG Kunbo, LIU Zhonghua, YU Lijun. Research on Flavors and Qualities of Optimization Blending Samples of Hunan Raw Dark Teas [J]. Journal of Tea Science, 2024, 44(5): 763-778.
[2]	CHEN Jiaxin, ZHANG Jinjia, ZUO Huiling, JIAO Yuhang, SHI Anhua. The Mechanism and Research Progress of Epigallocatechin Gallate in Improving Non-alcoholic Fatty Liver Disease [J]. Journal of Tea Science, 2024, 44(4): 543-553.
[3]	LEI Xiang, ZHANG Minfeng, LIN Hui, WANG Lili, ZHENG Deyong. Carbonylation Modification of Epicatechin and Its Activities of UV Absorption and Antioxidant [J]. Journal of Tea Science, 2024, 44(3): 493-500.
[4]	ZHOU Jinghui, LIU Changwei, ZHANG Sheng, XU Gang, XU Wei, HUANG Jian′an, LIU Zhonghua. Preparation of a Cross-linked Polyphenol Oxidase Aggregate Based on Catechins and Its Efficient Catalytic Synthesis of Theaflavin-3,3ʹ-digalate [J]. Journal of Tea Science, 2023, 43(3): 377-388.
[5]	CHEN Yifan, KAN Xinyi, JIANG Xiaolan, GAO Liping, XIA Tao. Physicochemical Properties of Tannase in Tea Plants (Camellia sinensis) and Its Application on Green Tea Beverages [J]. Journal of Tea Science, 2023, 43(1): 124-134.
[6]	MAN Ziyi, FENG Yi, WU Xiangting. Inhibitory Effect of Catechin Monomer EGC on Pancreatic Lipase and Mechanism [J]. Journal of Tea Science, 2022, 42(6): 863-874.
[7]	YU Rongxin, ZHENG Qinqin, CHEN Hongping, ZHANG Jinsong, ZHANG Xiangchun. Recent Advances in Catechin Biomedical Nanomaterials [J]. Journal of Tea Science, 2022, 42(4): 447-462.
[8]	WANG Yuyuan, LIU Renjian, LIU Shaoqun, SHU Canwei, SUN Binmei, ZHENG Peng. Expression Analysis and Functional Identification of CsTT2 R2R3-MYB Transcription Factor in Tea Plants [J]. Journal of Tea Science, 2022, 42(4): 463-476.
[9]	WU Qinyao, YANG Jiangfan, LIN Cheng, GUAN Xi. Research on the Changes of China's Tea Production Layout [J]. Journal of Tea Science, 2022, 42(2): 290-300.
[10]	LIU Yajun, WANG Peiqiang, JIANG Xiaolan, ZHUANG Juhua, GAO Liping, XIA Tao. Research Progress on the Biosynthesis of Monomeric and Polymeric Catechins in Camellia sinensis [J]. Journal of Tea Science, 2022, 42(1): 1-17.
[11]	MA Bingsong, WANG Jiacai, XU Chengcheng, REN Xiaoying, MA Cunqiang, ZHOU Binxing. Differences of Phenolic Components in Puer Raw Tea with Various Storage Periods and Their Effects on the in vitro Antioxidant Capacities [J]. Journal of Tea Science, 2022, 42(1): 51-62.
[12]	LYU Haowei, WU Chuanyu, TU Zheng, CHEN Jianneng, JIA Jiangming, CHEN Zhiwei, YE Yang. EDEM-based Optimization of Classification Parameters of Machine-picked Tea Fresh Leaf Vibratory Classifier [J]. Journal of Tea Science, 2022, 42(1): 120-130.
[13]	ZHOU Yufei, ZHOU Lin, YU Lijun, XU Shuai, YANG Yihuan. Visualization Analysis in Research Status of Theaflavins Based on CiteSpace [J]. Journal of Tea Science, 2022, 42(1): 131-139.
[14]	WU Wenliang, TONG Tong, HU Yao, ZHOU Hao, YIN Xia, ZHANG Shuguang. Camellia Ptilophylla and Specific Chemical Components, Theirs Health Beneficial Effects [J]. Journal of Tea Science, 2021, 41(5): 593-607.
[15]	ZHANG Jianyong, CHEN Lin, CUI Hongchun, WANG Weiwei, XUE Jinjin, XIONG Chunhua, JIANG Heyuan. Optimization of Technical Parameters for Chemical Synthesis of Theasinensin A by PBD and RSM [J]. Journal of Tea Science, 2020, 40(1): 51-62.

Study on the Optimum Condition of Synthesizing Theaflavins by Using Enzyme-catalysing Oxidation

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References 8

Related Articles 15

Metrics

Comments

Recommended 0