The Evaluation of the Stability of EGCG-Selenium Nanoparticles and Its Effect on Selenium Absorption and Utilization

WANG Le; LI Huan; LI Jiahao; CHEN Xuan; LI Xinghui; SUN Kang

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Journal of Tea Science ›› 2017, Vol. 37 ›› Issue (4) : 373-382.

The Evaluation of the Stability of EGCG-Selenium Nanoparticles and Its Effect on Selenium Absorption and Utilization

WANG Le, LI Huan, LI Jiahao, CHEN Xuan, LI Xinghui, SUN Kang^*

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Abstract

(-)-epigallocatechin-3-gallate (EGCG) dispersed Selenium nanoparticles (EGCG-SeNPs) were prepared using vitamin C (Vc) and EGCG as the redox agent and dispersant. Characteristics of EGCG-SeNPs, which were determined using transmission electron microscope (TEM) and zetasizer, were spherical in shape with a mean diameter of (35±0.12)βnm and -0.05βmV zeta potential. The particles were aggregated in strong acid and high temperature conditions (pH1.0 and 70℃), with the particle size increased by about 10 times. And, EGCG in EGCG-SeNPs had good stability as a dispersant. With the administration of 25, 50 and 100βμg·kg^-1 (Calculated with selenium content), selenium content in liver and kidney of 50 and 100βμg·kg^-1 sodium selenite and EGCG-SeNPs treated mice were significantly increased. Glutathione peroxidase (GPx) activity in serum, liver and kidney of all the treatment groups were significantly increased in a dose-dependent manner. However, there was no significant difference between sodium selenite and EGCG-SeNPs at the same dose on selenium content and GPx activity. Hence, it can be concluded that EGCG-SeNPs synthesized using Vc as the redox agent might have the similar bioavailability to sodium selenite.

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(-)-epigallocatechin-3-gallate / bioavailability / glutathione peroxidase activity / nanoparticle / selenium

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WANG Le, LI Huan, LI Jiahao, CHEN Xuan, LI Xinghui, SUN Kang. The Evaluation of the Stability of EGCG-Selenium Nanoparticles and Its Effect on Selenium Absorption and Utilization[J]. Journal of Tea Science. 2017, 37(4): 373-382

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