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Journal of Tea Science ›› 2020, Vol. 40 ›› Issue (6): 739-750.doi: 10.13305/j.cnki.jts.2020.06.003

• Research Paper • Previous Articles     Next Articles

Interaction Identification and Expression Analysis of CsCIPK12 and CsKIN10 in Tea Plant

FENG Xia, DI Taimei, PENG Jing, LI Nana, YAO Lina, YANG Yajun, WANG Xinchao*, WANG Lu*   

  1. Tea Research Institute, Chinese Academy of Agricultural Sciences / National Center for Tea Improvement/Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China
  • Received:2020-04-20 Revised:2020-05-13 Online:2020-12-15 Published:2020-12-10

Abstract: Sucrose non-fermenting 1-related protein kinase (SnRK) plays important roles in metabolic regulation and stress signal transduction. In this study, CsCIPK12 gene of the SnRK3 subfamily and CsKIN10 gene of the SnRK1 subfamily in tea plant were used as research objects. Protein sequence alignment and phylogenetic tree analysis showed that both CsCIPK12 and CsKIN10 have an N-terminal kinase domain and a C-terminal regulatory domain; CsCIPK12 has a NAF/FISL domain that binds to CBLs, and is homologous with AtCIPK12 in Arabidopsis thaliana and PtCIPK17, 18, and 19 in poplar; CsKIN10 has the ubiquitin-related UBA domain and is homologous with PtSnRK1 in poplar. Yeast two-hybrid analysis showed that CsCIPK12 could interact with CsKIN10. Expression analysis found that the expression of CsKIN10 was induced by natural cold acclimation to varying degrees in the three tea cultivars ‘Longjing 43’, ‘Zhenong 12’ and ‘Damianbai’, which was consistent with the results of the previous study on CsCIPK12. Under 4℃ cold stress, the expressions of CsCIPK12 and CsKIN10 were significantly up-regulated in mature leaves (the highest induction levels were 4-fold and 2.3-fold, respectively), while in the shoots, the responses of CsCIPK12 and CsKIN10 to the cold stress were not significant. Under ABA, glucose and sucrose treatments, the expression levels of CsCIPK12 and CsKIN10 were significantly up-regulated in mature leaves. These results suggest that CsCIPK12 interacts with CsKIN10, which are involved in ABA and sugar signaling pathways and may play an important role in cold response in tea plants.

Key words: tea plant, CIPK12/SnRK3.9, KIN10/SnRK1.1, cold stress, yeast two-hybrid

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