Analysis of Gene Expression Related to Anthocyanin Regulation of &#x02018;P113' Purple Strain of Camellia tachangensis F. C. Zhang in Different Seasons

LIU Xia; LI Fang; SONG Qinfei; NIU Suzhen; LYU Litang

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Journal of Tea Science ›› 2021, Vol. 41 ›› Issue (6) : 789-801.

Analysis of Gene Expression Related to Anthocyanin Regulation of ‘P113' Purple Strain of Camellia tachangensis F. C. Zhang in Different Seasons

LIU Xia, LI Fang, SONG Qinfei, NIU Suzhen^*, LYU Litang

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Abstract

In order to clarify the molecular mechanism of anthocyanin accumulation and the expression characteristics of related genes in the synthetic pathway of anthocyanin in Camellia tachangensis F. C. Zhang purple bud line P113 in different seasons, one bud and two leaves of P113 in spring, summer and autumn were analyzed by RNA-Seq and UHPLC-MS/MS. The results show that the 10 anthocyanin contents changed with seasons, and 4 of them were up-regulated with the change of spring, summer and autumn, which were consistent with leaf color phenotype of ‘P113'. The expression patterns of structural genes PAL, C4H, 4CL, CHS, F3H and F3'5'H were basically the same, which were up-regulated in summer, but not significantly changed in autumn. The two differentially expressed 4CL genes were up-regulated in summer. The gene expression patterns of F3H, DFR and ANS were similar, and their expressions were up-regulated in summer and down-regulated in autumn. One of the two F3'H genes was down-regulated in summer, and one was up-regulated with seasonal changes. The 13 genes of FLS were all up-regulated in summer, but they showed different expression patterns in autumn. The modifier gene LAR had two expression patterns in summer and autumn. There was only 1 differentially expressed gene in ANR that was up-regulated with seasonal changes. Among the three UGT79B1 genes, one was down-regulated in summer, up-regulated in autumn, and the rest two genes were up-regulated in summer, but not significantly changed in autumn. Seven differentially expressed CCoAOMT genes were up-regulated in summer. Two of them down regulated in autumn, and the rest five genes were not significant changed. The regulatory genes bHLH and MYB were up-regulated with seasonal changes, and had a positive relationship with anthocyanin biosynthesis. The results show that the expressions of structural genes, modifier genes and regulatory genes of ‘P113' (Camellia tachangensis F. C. Zhang) had certain time characteristics in different seasons, which led to the differences in anthocyanin accumulation.

Key words

Camellia tachangensis F. C. Zhang / anthocyanins / differentially expressed genes

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LIU Xia, LI Fang, SONG Qinfei, NIU Suzhen, LYU Litang. Analysis of Gene Expression Related to Anthocyanin Regulation of ‘P113' Purple Strain of Camellia tachangensis F. C. Zhang in Different Seasons[J]. Journal of Tea Science. 2021, 41(6): 789-801

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