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Journal of Tea Science ›› 2005, Vol. 25 ›› Issue (3): 225-228.doi: 10.13305/j.cnki.jts.2005.03.012

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Determination of Theanine in Tea by Reversed-Phase High Performance Liquid Chromatography-Evaporative Light-Scattering Detector

LI Yin-hua, LIU Zhong-hua*, HUANG Jian-an, GONG Yu-shun   

  1. Institute of Natural Products,Hunan Agricultural University, Changsha 410128, China
  • Online:2005-09-25 Published:2019-09-16

Abstract: High performance liquid chromatography (HPLC) coupled with evaporative light scattering detector(ELSD-2000)was used for the determination of theanine in tea without derivatization. A Polaris-C18 column (250βmm×4.6βmm) was used with the mobile phase of 0.1%trifluoroacetic acid water solution and 0.1% trifluoroacetic acid acetonitrile solution .The flow rate was at 0.5βml/min. The linear response ranged from 0.062~0.450βmg/mL for theanine. The recovery was 97.5%. The method was evaluated as accurate and simple.

Key words: HPLC, Evaporative light-scattering detector, Theanine, Tea

CLC Number: