Review
LU Mi, HAI Xingmei, YU Longfeng, WU Tian
α-Farnesene, a key sesquiterpene, modulates aroma quality and stress response in tea plants. α-Farnesene synthase (AFS), the rate-limiting terminal enzyme in its biosynthetic pathway, serves as a core molecular hub linking terpenoid metabolism, stress-resistant breeding, and quality regulation in Camellia sinensis. Based on the terpenoid secondary metabolic network of tea plants, this study systematically reviewed the cloning, identification, expression patterns, and biological functions of the α-farnesene synthase gene (CsAFS) in Camellia sinensis. It further described the synergistic regulatory network composed of transcription factors, phytohormones and environmental signals, and clarified the scientific implications and intrinsic crosstalk mechanisms of tea-specific regulatory modules involving CsAFS. Through cross-species comparative analysis, this paper revealed the evolutionary conservation and species-specific characteristics of CsAFS, and comprehensively dissected its biological roles in stress response, aroma formation and quality regulation. Meanwhile, potential applications of CsAFS in stress-resistant breeding, ecological tea garden construction, and tea processing quality improvement were discussed. In view of current research gaps, such as incomplete functional characterization of gene family members, immature genetic transformation systems, and insufficient compatibility between regulatory strategies and practical tea production, future research priorities were proposed based on cutting-edge advances in tea science. This study aimed to provide theoretical foundations for stress-resistant breeding, ecological cultivation, and quality improvement in tea plants.