利用EST-SSR分析江北茶区茶树资源的遗传多样性和遗传结构

doi:10.13305/j.cnki.jts.2009.3.011

茶叶科学 ›› 2009, Vol. 29 ›› Issue (3): 243-250.doi: 10.13305/j.cnki.jts.2009.3.011

• • 上一篇

利用EST-SSR分析江北茶区茶树资源的遗传多样性和遗传结构

姚明哲^1,2, 刘振², 陈亮^2,*, 王新超², 马春雷², 梁月荣^1,*

1. 浙江大学农业与生物技术学院,浙江杭州310029;
2. 中国农业科学院茶叶研究所/国家茶树改良中心,浙江杭州310008

收稿日期:2009-02-17 修回日期:2009-03-16 出版日期:2009-06-15 发布日期:2019-09-06
通讯作者: *
作者简介:姚明哲(1975— ),男,山东成武人,在职博士生,主要从事茶树资源育种研究。
基金资助:
国家863计划(2006AA10Z171)、浙江省“钱江人才”计划(2006R10042)和浙江省自然科学基金(Y305124、Y305105)

Genetic Diversity and Structure of Tea Germplasm Originated from Region of North Yangtze River Based on EST-SSR Markers

YAO Ming-zhe^1,2, LIU Zhen², CHEN Liang^2,*, WANG Xin-chao², MA Chun-lei², LIANG Yue-rong^1,*

1. College of Agriculture and Biological Technology, Zhejiang University, Hangzhou 310029, China;
2. National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences, Hangzhou 310008, China

Received:2009-02-17 Revised:2009-03-16 Online:2009-06-15 Published:2019-09-06

摘要/Abstract

摘要： 利用25对EST-SSR引物对江北茶区的45份茶树初级核心种质的遗传多样性、遗传结构和亲缘关系进行了分析。25对引物共检测到83个等位位点,平均每对引物可检测到等位位点3.3个,可鉴定的基因型为6个。引物的PIC值平均为0.61,扩增位点的观测杂合度高于期望杂合度。45份供试种质中可观测的等位位点平均为4.2个,有效等位位点为2.8个。等位位点观测杂合度平均为0.73,基因多样性指数为0.61,Shannon信息指数为1.11。江北茶区主要省份间茶树种质的遗传分化程度较低（G_st=0.2）,而基因流(N_m=3.9)较高。AMOVA分析显示,95.97%的变异发生于居群内。45份供试种质间的遗传相似系数在0.32~0.89之间,聚类分析表明供试资源在亲缘关系上未表现出明显的地区分化。湖北、安徽和陕西三个主要省份茶树种质间的遗传距离平均为0.048,其中陕西资源在亲缘关系上略远于湖北和安徽。

关键词: 茶树, EST-SSR, 遗传多样性, 遗传结构

Abstract: The 25 pairs of EST-SSR primers were used to analyze the genetic diversity, genetic structure and genetic relationship of 45 tea accessions originated from region of north Yangtze River. Totally 83 alleles were identified, on an average of 3.3 alleles each pair of primers. The mean of polymorphism information content (PIC) was 0.61. The observed heterozygosity (Ho) was averagely higher than the expected heterozygosity (He). Among 45 tea accessions the average numbers of the observed alleles were 4.2, and the number of effective alleles 2.8. The observed heterozygosity, gene diversity and Shannon information index were 0.73, 0.61 and 1.11, respectively. The low genetic difference （Gst=0.2） and high geneflow (Nm=3.9) of tea populations from Hubei, Anhui and Shaanxi were found. Analysis of molecular variance (AMOVA) showed that variance components inter-population and intra-population were 4.03% and 95.97% respectively. The paired similarity coefficients of 45 accessions were from 0.32 to 0.89. No clearly regional partition among 45 accessions was found from the dendrogram. The high level of genetic similarity was demonstrated among tea population of Hubei, Anhui and Shaanxi province, and Shaanxi population showed relatively distant relationship comparing to population of Hubei and Anhui.

Key words: tea, EST-SSR, genetic diversity, genetic structure, Camellia sinensis

中图分类号:

S571.1
Q311

姚明哲, 刘振, 陈亮, 王新超, 马春雷, 梁月荣. 利用EST-SSR分析江北茶区茶树资源的遗传多样性和遗传结构[J]. 茶叶科学, 2009, 29(3): 243-250. doi: 10.13305/j.cnki.jts.2009.3.011.

YAO Ming-zhe, LIU Zhen, CHEN Liang, WANG Xin-chao, MA Chun-lei, LIANG Yue-rong. Genetic Diversity and Structure of Tea Germplasm Originated from Region of North Yangtze River Based on EST-SSR Markers[J]. Journal of Tea Science, 2009, 29(3): 243-250. doi: 10.13305/j.cnki.jts.2009.3.011.

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利用EST-SSR分析江北茶区茶树资源的遗传多样性和遗传结构

Genetic Diversity and Structure of Tea Germplasm Originated from Region of North Yangtze River Based on EST-SSR Markers

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