茶叶科学 ›› 2018, Vol. 38 ›› Issue (2): 162-173.doi: 10.13305/j.cnki.jts.2018.02.007
张玥, 胡雲飞, 王树茂, 柯子星, 林金科*
收稿日期:
2017-10-12
修回日期:
2017-11-03
出版日期:
2018-04-15
发布日期:
2019-08-28
通讯作者:
*ljk213@163.com
作者简介:
张玥,女,讲师,博士研究生,主要从事茶树分子生物学研究,yaoyao86527@163.com。
基金资助:
ZHANG Yue, HU Yunfei, WANG Shumao, KE Zixing, LIN Jinke*
Received:
2017-10-12
Revised:
2017-11-03
Online:
2018-04-15
Published:
2019-08-28
摘要: 从茶树(Camellia sinensis)转录组数据库中获得6个与儿茶素合成相关的MYB转录因子,对其进行蛋白理化性质与结构功能预测、核定位信号和蛋白保守结构域分析,通过同源分析构建系统发育树。结果表明,与儿茶素合成相关的6个MYB蛋白都属于亲水性的非分泌蛋白,定位在叶绿体、线粒体和细胞核上,其空间结构主要是α-螺旋和β-转角;保守结构域的分析发现除了comp159173_c0基因,其余5个都属于SANT超家族基因成员。系统进化树显示6个MYB蛋白形成4个分支,显示出各自间较远的亲缘关系。差异表达分析的结果进一步证实了6个MYB转录因子与儿茶素合成代谢的相关性。
中图分类号:
张玥, 胡雲飞, 王树茂, 柯子星, 林金科. 茶树儿茶素合成相关MYB转录因子生物信息学分析[J]. 茶叶科学, 2018, 38(2): 162-173. doi: 10.13305/j.cnki.jts.2018.02.007.
ZHANG Yue, HU Yunfei, WANG Shumao, KE Zixing, LIN Jinke. Bioinformatic Analysis of MYB Transcription Factors Involved in Catechins Biosynthesis in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science, 2018, 38(2): 162-173. doi: 10.13305/j.cnki.jts.2018.02.007.
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