Journal of Tea Science ›› 2015, Vol. 35 ›› Issue (6): 596-604.doi: 10.13305/j.cnki.jts.2015.06.013
Previous Articles Next Articles
XIE Xiaofang1,2, TIAN Xianfeng1, JIANG Changjun1,2, LI Yeyun1,*
Received:
2015-06-26
Revised:
2015-10-10
Online:
2015-12-15
Published:
2019-08-26
CLC Number:
XIE Xiaofang, TIAN Xianfeng, JIANG Changjun, LI Yeyun. Screening of microRNA Reference Genes for Real-time Fluorescence Quantitative PCR under Cold Stress in Camellia sinensis[J]. Journal of Tea Science, 2015, 35(6): 596-604.
[1] | Heid CA, Stevens J, Livak KJ, et al. Real time quantitative PCR[J]. Genome Res, 1996, 6(10): 986-994. |
[2] | Bustin SA, Benes V, Nolan T, et al. Quantitative real-time RT-PCR-a perspective[J]. J Mol Endocrinol, 2005, 34(3): 597-601. |
[3] | Ginzinger DG.Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream[J]. Exp Hematol, 2002, 30(6): 503-512. |
[4] | Klein D.Quantification using real-time PCR technology: applications and limitations[J]. Trends Mol Med, 2002, 8(6): 257-260. |
[5] | Dheda K, Huggett JF, Bustin SA, et al. Validation of housekeeping genes for normalizing RNA expression in real-time PCR[J]. Biotechniques, 2004, 37(1): 112-119. |
[6] | Chi XY, Hu RB, Yang QL, et al. Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR[J]. Mol Genet Genomics, 2012, 287(2): 167-176. |
[7] | Lu SF, Sun YH, Shi R, et al. Novel and mechanical stress-responsive microRNAs in Populus trichocarpa that are absent from Arabidopsis[J]. The Plant Cell Online, 2005, 17(8): 2186-2203. |
[8] | Zanca AS, Vicentini R, Ortiz-Morea FA, et al. Identification and expression analysis of microRNAs and targets in the biofuel crop sugarcane[J]. BMC Plant Biol, 2010, 10(1): 260. |
[9] | Thellin O, Zorzi W, Lakaye B, et al. Housekeeping genes as internal standards: use and limits[J]. J Biotechnol, 1999, 75(2): 291-295. |
[10] | Jain M, Nijhawan A, Tyagi AK, et al. Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR[J]. Biochem Biophys Res Commun, 2006, 345(2): 646-651. |
[11] | 韦朝领, 李叶云, 江昌俊. 茶树逆境生理及其分子生物学研究进展[J]. 安徽农业大学学报, 2009, 36(3): 335-339. |
[12] | 王新超, 杨亚军. 茶树抗性育种研究现状[J]. 茶叶科学, 2003, 23(2): 94-98. |
[13] | 李钱峰, 蒋美艳, 于恒秀, 等. 水稻胚乳RNA定量RT-PCR分析中参照基因选择[J]. 扬州大学学报: 农业与生命科学版, 2008, 29(2): 61-66. |
[14] | Peltier HJ, Latham GJ.Normalization of microRNA expression levels in quantitative RT-PCR assays: identification of suitable reference RNA targets in normal and cancerous human solid tissues[J]. Rna, 2008, 14(5): 844-852. |
[15] | Szabo A, Perou CM, Karaca M, ,et al. Statistical modeling for selecting housekeeper genes [J]. Genome Biol. Statistical modeling for selecting housekeeper genes [J]. Genome Biol, 2004, 5(8): R59.1-R59.10. |
[16] | Spanakis E.Problems related to the interpretation of autoradiographic data on gene expression using common constitutive transcripts as controls[J]. Nucleic Acids Res, 1993, 21(16): 3809-3819. |
[17] | 付媛媛, 穆春生, 高洪文, 等. 紫花苜蓿18 S rRNA基因的克隆及内参基因表达稳定性评价[J]. 植物生理学报, 2014, 50(12): 1809-1815. |
[18] | 樊连梅, 王超, 刘更森, 等. 苹果着色期实时定量PCR内参基因的筛选和验证[J]. 植物生理学报, 2014, 50(12): 1903-1911. |
[19] | 严海东, 蒋晓梅, 张新全, 等. 非生物胁迫下多年生黑麦草qRT-PCR分析中内参基因的选择[J]. 农业生物技术学报, 2014, 22(12): 1494-1501. |
[20] | Hao XY, Horvath DP, Chao WS, et al. Identification and evaluation of reliable reference genes for quantitative real-time PCR analysis in tea plant (Camellia sinensis (L.) O. Kuntze)[J]. Int J Mol Sci, 2014, 15(12): 22155-22172. |
[21] | Monzo M, Navarro A, Bandres E, et al. Overlapping expression of microRNAs in human embryonic colon and colorectal cancer[J]. Cell Res, 2008, 18(8): 823-833. |
[22] | Schaefer A, Jung M, Miller K, et al. Suitable reference genes for relative quantification of miRNA expression in prostate cancer[J]. Exp Mol Med, 2010, 42(11): 749-758. |
[23] | Wotschofsky Z, Meyer HA, Jung M, et al. Reference genes for the relative quantification of microRNAs in renal cell carcinomas and their metastases[J]. Anal Biochem, 2011, 417(2): 233-241. |
[24] | Vandesompele J, Preter KD, Pattyn F, ,et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes [J]. Genome Biol. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes [J]. Genome Biol, 2002, 3(7): research0034.1-0034.11. |
[1] | GUO Yongchun, WANG Pengjie, CHEN Di, ZHENG Yucheng, CHEN Xuejin, YE Naixing. Genome-wide Identification and Expression Analysis of SRO Gene Family in Camellia sinensis [J]. Journal of Tea Science, 2019, 39(04): 392-402. |
[2] | ZHENG Shizhong, JIANG Shengtao, LIU Wei, CHEN Meixia, LIN Yuling, LAI Zhongxiong, LIN Jinke. Cloning and Functional Analysis of the CsMYB Promoter In Tea Plant (Camellia sinensis L.) [J]. Journal of Tea Science, 2018, 38(6): 580-588. |
[3] | PANG Dandan, ZHANG Fen, ZHANG Yazhen, WEI Kang, WANG Liyuan, CHENG Hao. Research Advance on Biosynthesis, Regulation and Function of Anthocyanins in Tea Plant [J]. Journal of Tea Science, 2018, 38(6): 606-614. |
[4] | SHAN Ruiyang, LIN Zhenghe, CHEN Zhihui, ZHONG Qiusheng, YOU Xiaomei, CHEN Changsong. Molecular Cloning and Expression Analysis of Cytochrome P450 CYP71A26 and CYP71B34 Genes in Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2018, 38(5): 450-460. |
[5] | GAN Yudi, SUN Kang, LI Huijuan, DU Zhongying, ZHAO Zhen, PANG Xing, LI Xinghui, CHEN Xuan. Effect of Two Prokaryotic Expressed Vectors on the Activity of PPO from Camellia sinensis [J]. Journal of Tea Science, 2018, 38(4): 396-405. |
[6] | RAN Wei, ZHANG Jin, ZHANG Xin, LIN Songbo, SUN Xiaoling. Infestation of Ectropis obliqua Affects the Catechin Metabolism in Tea Plants [J]. Journal of Tea Science, 2018, 38(2): 133-139. |
[7] | YE Xiaoli, PAN Junting, ZHU Jiaojiao, SHU Zaifa, CUI Chuanlei, XING Anqi, NONG Shouhua, ZHU Xujun, FANG Wanping, WANG Yuhua. Cloning and Expression Analysis of Small GTPase (CsRAC5) under Cold Stress in Tea Plant (Camellia sinensis) [J]. Journal of Tea Science, 2018, 38(2): 146-154. |
[8] | ZHANG Yongheng, WANG Siqing, CHEN Jiangfei, WANG Weidong, ZHOU Tianshan, XIAO Bin, YANG Yajun, YU Youben. Cloning and Expression Analysis of CsSnRK2.1 and CsSnRK2.2 Genes in Tea Plant (Camellia sinensis) under Abiotic Stress [J]. Journal of Tea Science, 2018, 38(2): 183-192. |
[9] | YU Xinlei, AI Yujie, QU Fengfeng, AI Zeyi, LIU Shuyuan, CHEN Yuqiong, NI Dejiang. Metabolomics Application in the Study of Tea Quality Formation [J]. Journal of Tea Science, 2018, 38(1): 20-32. |
[10] | LIN Weidong, CHEN Zhidan, SUN Weijiang, YANG Ruxing. Analysis of Genetic Diversity of Fujian Tea Varieties by SCoT Markers [J]. Journal of Tea Science, 2018, 38(1): 43-57. |
[11] | SHEN Wei, TENG Ruimin, LI Hui, LIU Zhiwei, WANG Yongxin, WANG Wenli, ZHUANG Jing. Cloning of a MADS-box Transcription Factor Gene from Camellia sinensis and its Response to Abiotic Stresses [J]. Journal of Tea Science, 2017, 37(6): 575-585. |
[12] | GUO Junhong, WANG Weidong, GU Xing, GUO Shasha, GAO Yuefang, YANG Yajun, XIAO Bin. Cloning and Expression Analysis of WRKY Transcription Factor Gene CsWRKY57 in Tea Plant (Camellia sinensis) [J]. Journal of Tea Science, 2017, 37(4): 411-419. |
[13] | LI Hailin, WANG Liyuan, CHENG Hao, WEI Kang, RUAN Li, WU Liyun. The Effects of Nitrogen Supply on Agronomic Traits and Chemical Components of Tea Plant [J]. Journal of Tea Science, 2017, 37(4): 383-391. |
[14] | WANG Rangjian, YANG Jun, KONG Xiangrui, GAO Xiangfeng. Genetic Analysis of Full- and Half-sib Families of Tea Cultivar Jinguanyin Based on SSR Molecular Markers [J]. Journal of Tea Science, 2017, 37(2): 139-148. |
[15] | LU Cui, SHEN Chengwen. Research Progress of Albino Tea Plant Camellia sinensis (L.) O. Kuntze) [J]. Journal of Tea Science, 2016, 36(5): 445-451. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||
|