The Release of Volatiles in Resistant and Susceptible Tea Cultivars under Empoasca Onukii Feeding

REN Qianqian; ZHUANG Mingzhu; CAI Xiaoming; BIAN Lei; LUO Zongxiu; LI Zhaoqun; YOU Minsheng; CHEN Zongmao; JIN Shan

doi:10.13305/j.cnki.jts.2020.06.008

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Journal of Tea Science ›› 2020, Vol. 40 ›› Issue (6) : 795-806. DOI: 10.13305/j.cnki.jts.2020.06.008

Research Paper

The Release of Volatiles in Resistant and Susceptible Tea Cultivars under Empoasca Onukii Feeding

REN Qianqian¹, ZHUANG Mingzhu¹, CAI Xiaoming², BIAN Lei², LUO Zongxiu², LI Zhaoqun², YOU Minsheng³, CHEN Zongmao^2,*, JIN Shan^1,*

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Abstract

In order to study the volatile release and the relative metabolism induced by small green leafhoppers (Empoasca onukii) feeding in tea plants, the resistant tea cultivar (JY) and susceptible tea cultivar (EB) were used as materials. The time course (6, 12, 24, 36, 48, 72βh) of tea volatile compounds released by tea plants under E. onukii feeding were collected and detected by using dynamic headspace collection method and GC-MS technology. An integrative analysis of the metabolism and transcriptome data was then performed, and the target genes in the synthetic pathway of the main volatiles were screened and analyzed. The results show that healthy tea plants released less volatiles. While ten main volatiles such as β-Ocimene, DMNT, Linalool, Farnesene, etc. were identified in tea plants under E. onukii feeding. Among them, the contents of monoterpenoids were higher in the susceptible tea cultivar EB, and sesquiterpenoids were higher in the resistant tea cultivar JY. Furthermore, transcriptome data show that the key genes in terpene synthesis pathway were up-regulated in both cultivars after E. onukii feeding. The expression levels of genes related to the synthesis of monoterpenoids were relatively higher in susceptible tea cultivar than those in resistant cultivar. However, no significant expression differences were identified in genes related to sesquiterpenes synthesis in resistant and susceptible tea cultivars. The results of this study provided a theoretical basis for the tea resistance mechanism and green control of leafhopper in tea plantation.

Key words

Empoasca onukii / gene regulation / tea plant / terpenoids / volatiles

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REN Qianqian, ZHUANG Mingzhu, CAI Xiaoming, BIAN Lei, LUO Zongxiu, LI Zhaoqun, YOU Minsheng, CHEN Zongmao, JIN Shan. The Release of Volatiles in Resistant and Susceptible Tea Cultivars under Empoasca Onukii Feeding[J]. Journal of Tea Science. 2020, 40(6): 795-806 https://doi.org/10.13305/j.cnki.jts.2020.06.008

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