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Journal of Tea Science ›› 2017, Vol. 37 ›› Issue (2): 149-159.

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Cloning and Expression Analysis of Phosphoenolpyruvate Transporter Gene CsPPT2 in Tea Plant(Camellia sinensis)

JI Zhifang1, GAN Yudi1, CHEN Changsong2, YANG Dingjun1, SUN Kang1, LI Xinghui1, CHEN Xuan1,*   

  1. 1. Tea Research Institute, Nanjing Agricultural University, Nanjing 210095, China;
    2. Tea Research Institute, Fujian Academy of Agricultural Sciences, Fu′an 355015, China;
  • Received:2017-02-14 Revised:2017-02-22 Online:2017-04-15 Published:2019-08-22

Abstract: A full length cDNA sequence of phosphoenolpyruvate transporter gene (CsPPT2) was obtained from tea plant(Camellia sinensis) cultivar ‘Baiye 1’ by polymerase chain reaction (PCR) and rapid amplification of cDNA ends PCR(RACE-PCR). The nucleotide sequence length of this gene was 1469 bp, containing a complete open reading frame (1218 bp) encoding 406 amino acids. Bioinformatic analysis showed that the predicted molecular weight of the protein was 44.6 kD, and the theoretic isoelectric point was 9.90. CsPPT2 has 6 trans-membrane regions, which may belong to the hydrophobic chloroplast trans-membrane protein. Phylogenetic tree analysis showed the phosphoenolpyruvate transporter family in tea plant could be divided into two subgroups, with CsPPT1 and CsPPT2 belonged to different subgroups. The expression of CsPPT1 and CsPPT2 were compared in different periods and plant organs. It showed that CsPPT2 was expressed in all tested tissues, with higher expression level in roots and mature leaves than those of CsPPT1. But the expression level of CsPPT1 in young shoots was higher than CsPPT2. CsPPT2 was transiently elevated at the beginning of the albino, but inhibited during the development of albino, indicates that the inhibition of the expression of CsPPT2 may act as a key factor for low-catechins and high-amino acids in ‘Baiye 1’.

Key words: Camellia sinensis, Baiye 1, phosphoenolpyruvate transporter, CsPPT2, expression analysis

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