红茶菌菌液对亚硝酸盐降解及NO的生成的影响

俸毅; 蒋盛军; 王超; 罗长辉; 但忠

doi:10.13305/j.cnki.jts.2009.2.002

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茶叶科学 ›› 2009, Vol. 29 ›› Issue (2) : 89-94. DOI: 10.13305/j.cnki.jts.2009.2.002

红茶菌菌液对亚硝酸盐降解及NO的生成的影响

俸毅, 蒋盛军^*, 王超, 罗长辉, 但忠

作者信息 +

Study on Degradation of Nitrite by Kombucha Tea

FENG Yi, JIANG Sheng-jun^*, WANG Chao, LUO Chang-hui, DAN Zhong

Author information +

文章历史 +

摘要

为了探索红茶菌菌液预防胃肠癌的保健机理,初步研究了红茶菌菌液对亚硝酸盐的降解效果。结果表明：红茶菌菌液能够快速降解亚硝酸盐,同时在降解过程中产生大量的NO。在红茶菌菌液中添加100~1 000 mg/L的亚硝酸钠,红茶菌菌液在2 h内的降解量可达到89.02~431.16 mg/L;在红茶菌菌液中添加5~200 mg/L的亚硝酸钠,红茶菌菌液在20 min内可产生52.3~376.1 mg/m³浓度的NO。

Abstract

In order to explore the health-protecting mechanism of Kombucha tea in the preventing of gastrointestinal cancer and other diseases, degradation of nitrite in vitro by Kombucha tea was preliminarily studied. The results showed that Kombucha tea could efficiently degrade nitrite in vitro and generate a lot of NO. 100~1 000 mg/L of sodium nitrite were added in the Kombucha tea, the degradation of nitrite by kombucha tea was up to 89.02~431.16 mg/L in 2 h. 5~200 mg/L of sodium nitrite could be efficiently degraded by kombucha tea, which could generate 52.3~376.1 mg/m³ of NO in 20 mins.

导出引用

俸毅, 蒋盛军, 王超, 罗长辉, 但忠. 红茶菌菌液对亚硝酸盐降解及NO的生成的影响[J]. 茶叶科学. 2009, 29(2): 89-94 https://doi.org/10.13305/j.cnki.jts.2009.2.002

FENG Yi, JIANG Sheng-jun, WANG Chao, LUO Chang-hui, DAN Zhong. Study on Degradation of Nitrite by Kombucha Tea[J]. Journal of Tea Science. 2009, 29(2): 89-94 https://doi.org/10.13305/j.cnki.jts.2009.2.002

中图分类号： S571.1 Q939.14+9

参考文献

[1] LIU C H, HSU W H, LEE F L, et al. The isolation and identification of microbes from a fermented tea beverage, Haipao, and their interactions during Haipao fermentation[J]. Food Microbiology, 1996, 8(13): 407~415.
[2] 吴微, 盖宝川, 籍宝平. 红茶菌混合菌种的分离与鉴定[J]. 食品科学, 2004, 3(25): 55~58.
[3] GREENWALT C J, STEINKRAUS K H, LEDFORD R A.Kombucha, the fermented tea: microbiology, composition, and claimed health effects[J]. Food Protect, 2000, 22(63): 976~981.
[4] BLANC P J.Characterization of the tea fungus metabolites[J]. Biotechnology Letter, 1996, 87(18): 139~142.
[5] SIEVERS M, ALONSO L, GIANOTTI S, et al. 16S-23S ribosomal RNA spacer regions of Acetobacter europaeus and A. xylinium, tRNA genes and antitermination sequences[J]. FEMS Microbiology Letter, 1996, 48(142): 43~48.
[6] YAMADAY, HOSHINO K, ISHIKAWA T.The phylogeny of acetic acid bacteria based on the partial sequences of 16S ribosom-al RNA: the elevation of the subgenus Gluconoacetobacter to the generic level[J]. Biosci Biotech Biochem, 1997, 33(61): 1244~1251.
[7] Greenwalt C J, Steinkraus K H, Ledford R A.Kombucha, the fermented tea: microbiology, composition, and claimed health effects. Food Protect, 2000, 63(7): 976~981.
[8] Deng D J.Progress of gastric cancer etiology: N-nitrosamides in the 1990s[J]. World J Gastroenterology, 2000, 6: 613~618.
[9] Kono Y, Shibata H, Kodama Y, et al. The suppression of N-Nitrosating reaction by chlorogenic acid[J]. Biochem J, 1995, 312(pt3): 947~953.
[10] 张庆芳, 迟乃玉, 郑燕, 等. 乳酸菌降解亚硝酸盐机理的研究[J]. 食品与发酵工业, 2002, 28(8): 27~31.
[11] Dykhuizen R S, Fraser A, McKenzie H, et al. Helicobacter pylori is killed by nitrite under acidic conditions. Gut, 1998, 42(3): 334~337.
[12] Xia D S, Liu Y, Zhang C M, et al. Antimicrobial effect of acidified nitrate and nitrite on six common oral pathogens in vitro[J]. China Medical J, 2006, 119(22): 1904~1909.
[13] Sreeramulu G, Zhu Y, Knol W.Characterization of anti- microbial activity in Kombucha fermentation[J]. Acta Biotechnol, 2001, 21: 49~56.
[14] Benjamin N, Driscoll F O, Dougall H, et al. Stomach NO synthesis[J]. Nature, 1994, 368(7): 502.
[15] 田亚平. 亚硝酸盐是一种重要的EDRF调节因子[J]. 生物化学与生物物理学报, 1999, 31(2): 133~137.
[16] 田亚平, 王成彬, 童红莉, 等. 亚硝酸盐介导的降压效应及其机制探讨[J]. 科学通报, 2002,47: 41~43.
[17] Kondo S, Tayama K, Tsukamoto Y, et al. Antihypertensive effects of acetic acid and vinegar on spontaneously hypertensive rats. Biosci Biotechnol Biochem. 2001, 65(12): 2690.
[18] Currier R W, Goddard J, Buechler K, ,et al. Unexplained Severe illness Possibly Associated with Consumption of Kombucha Tea—Iowa[J]. MMWR. Unexplained Severe illness Possibly Associated with Consumption of Kombucha Tea—Iowa[J]. MMWR, 1995, 44(48): 892~893, 899~890.
[19] Srinivasan R, Smolinske S, Greenbaum D.Probable gastrointestinal toxicity of Kombucha tea: is this beverage healthy or harmful?[J]. J Gen Intern Med, 1997, 12(10): 643~644.