昆虫刺探电位图谱（EPG）技术在茶树抗刺吸式口器害虫研究中的应用

doi:10.13305/j.cnki.jts.2012.05.008

摘要/Abstract

摘要： 以假眼小绿叶蝉为主的茶园刺吸式昆虫是茶叶生产中的重要害虫,其特殊的产卵习性和危害方式使化学农药很难发挥理想的作用。茶树品种抗虫性研究作为控制刺吸式害虫的一种新途径,引起了科研工作者的关注和重视。而昆虫刺探电位图谱（Electrical Penetration Graph,EPG）技术,作为刺吸式口器昆虫的取食行为记录仪,参与到这一研究,明确了许多叶蝉行为和品种抗虫性间的关系。本文在分析EPG技术特点的基础上,总结了EPG技术在茶园刺吸式害虫的取食行为、茶树品种抗虫性的检测和抗性机理方面的应用,并且对相关研究结果进行梳理,归纳出假眼小绿叶蝉的取食过程和特点。

关键词: 刺探电位图谱, 刺吸式昆虫, 茶树抗虫性, 取食行为

Abstract: Piercing-sucking insects including green leafhopper are the important pest in tea industry. The special way of oviposition and damage of green leafhopper results in that the chemical pesticides were difficult to play their desired role. Tea cultivar resistance, as a part for pset management, gained much attentions and concerns. As the recorder of feeding behaviors of piercing-sucking insects, EPG technique was extensively involved in the cultivar resistance research. Firstly the characteristics of EPG technique were analyzed in present paper, and especially the applications of EPG in the investigation of relationship between the feeding behaviors of Piercing-sucking insects, and the resistance of tea plant to leafhopper were investigated. Furthermore, the paper recorded the feeding process and characteristics of green leafhopper based on the relative results.

Key words: electrical penetration graphs, piercing-sucking insects, tea plant resistance, feeding behavior

中图分类号:

金珊, 孙晓玲, 陈宗懋, 肖斌, 高宇, 边磊, 罗宗秀, 张正群. 昆虫刺探电位图谱（EPG）技术在茶树抗刺吸式口器害虫研究中的应用[J]. 茶叶科学, 2012, 32(5): 393-401. doi: 10.13305/j.cnki.jts.2012.05.008.

JIN Shan, SUN Xiao-ling, CHEN Zong-mao, XIAO Bin, GAO Yu, BIAN Lei, LUO Zong-xiu, ZHANG Zheng-qun. Applications of Electric Penetration Graph (EPG) Technique in the Research of Resistance of Tea Plant to Piercing-sucking Insects[J]. Journal of Tea Science, 2012, 32(5): 393-401. doi: 10.13305/j.cnki.jts.2012.05.008.

参考文献

[1] 杨青, 余德亿. 茶园假眼小绿叶蝉种群的生态调控[J]. 福建茶叶, 2008(2): 32-35.
[2] 张衍, 陈佳保. 安溪县不同海拔茶假眼小绿叶蝉的发生与综防措施[J]. 茶叶科学技术, 2006(2): 43.
[3] 徐金汉, 王念武, 张灵玲, 等. 假眼小绿叶蝉防治指标的研究[J]. 茶叶科学, 2005, 25(2): 131-135.
[4] 曹良俊, 祝勇武. 蚜虫的危害及防治[J]. 浙江林业, 2009(6): 33.
[5] 郭蕾, 邱宝利, 吴洪基, 等. 黑刺粉虱的发生、为害及其生物防治国内研究概况[J]. 昆虫天敌, 2007, 29(3): 123-128.
[6] 卓仁. 茶黑刺粉虱发生及防治方法[J]. 福建农业, 2004(6): 21.
[7] 徐金汉, 关瑞峰, 李春光, 等. 不同茶园昆虫群落结构及动态[J]. 华东昆虫学报, 2005, 14(4): 315-319.
[8] Robert L M, William H L.Introduction to insect pest management[M]. Canada: John Wiley﹠Sons, Inc. 1994: 73-128.
[9] Tjallingii W F, Minks A K, Harrewijn P.Electrical recording of stylet penetration activities In Aphids, their Biology, Natural Enemies and Control[J]. Amsterdam: Elsevier Science publishers, 1988, 2B: 95-107.
[10] 雷宏, 徐汝梅. EPG——一种研究植食性刺吸式昆虫刺探行为的有效方法[J]. 昆虫知识, 1996, 33(2): 116-120.
[11] 胡想顺, 刘小凤, 赵惠燕. 刺探电位图谱(EPG)技术的原理与发展[J]. 植物保护, 2006, 32(3): 1-4.
[12] 姜永幸, 郭予元. EPG技术在刺吸式昆虫取食行为研究中的应用[J]. 植物保护, 1994, 2: 33-35.
[13] Brodbeck B V, Mizell R F, French W J, et al. Amino acids as determinants of host preference for the xylem feeding leafhopper, Homalodisca coagulata(Homoptera: Cicadellidae)[J]. Oecologia, 1990, 83: 338-345.
[14] Gabrys B, Tjallingii W F, vgn Beek T A. Analysis of EPG recorded probing by cabbage aphid on host plant parts with different glucosinolate contents[J]. Journal of Chemical Ecology, 1997, 23(7): l66l-l 673.
[15] 阎凤鸣. 芥子油苷在甘蓝蚜寄主部位选择行为中的作用[J]. 昆虫学报, 2000, 43(3): 297-304.
[16] 韩宝瑜, 陈宗懋. 茶蚜在茶树不同部位上刺探行为的差异[J]. 植物保护学报, 2001, 28(1): 7-11.
[17] Jean M L, Martine G, Martial G, et al. Electrical penetration graphs from Cicadulina mbila on maize, the fine structure of its stylet pathways and consequences for virus transmission efficiency[J]. Entomologia Experimentalis et Applicata, 2001, 101(2): 93-109.
[18] 雷宏, 徐汝梅. 温室白粉虱取食行为的刺探电位(EPG)研究[J]. 昆虫学报, 1998, 41(2): 113-123.
[19] Tjallingii W F.Electronic recording of penetration behavior by aphids[J]. Entomologia Experimentalis et Applicata, 1978, 24(3): 721-730.
[20] Van Helden M, Tjallingii W F.Tissue localization of lettuce resistance to the aphid Nasonovia ribisnigri using electrical penetration graphs[J]. Entomologia Experimentalis et Applicata, 1993, 68(3): 269-278.
[21] Seo B Y, Kwon Y H, Jung J K, et al. Electrical penetration graphic waveforms in relation to the actual positions of the stylet tips of Nilaparvata lugens in rice tissue[J]. Journal of Asia-Pacific Entomology, 2009, 12(2): 89-95.
[22] Kindt F, Joosten N N, Peters D, et al.. Characterisation of the feeding behaviour of western flower thrips in terms of electrical penetration graph (EPG) waveforms[J]. Journal of Insect Physiology, 2003, 49(3): 183-191.
[23] 苗进, 韩宝瑜. 假眼小绿叶蝉(Empoasca vitis Gothe)在不同品种茶树上的取食行为[J]. 生态学报, 2007, 27(10): 3973-3982.
[24] 刘丽芳, 徐德良, 穆丹, 等. EPG技术分析不同品种茶树抗假眼小绿叶蝉取食行为的差异[J]. 安徽农业大学学报, 2011, 38(2): 146-150.
[25] Jin S, Chen Z M,Elaine A.Backus, et al. Characterization of EPG waveforms for the tea green leafhopper, Empoasca vitis Göthe (Hemiptera: Cicadellidae), on tea plants and their correlation with stylet activities[J]. Journal of insect physiology(待录用).
[26] 金珊, 孙晓玲, 陈宗懋, 等. 不同茶树品种对假眼小绿叶蝉抗性的研究[J]. 中国农业科学, 2012, 45(2): 255-265.
[27] 胡想顺, 赵惠燕, 胡祖庆, 等. 禾谷缢管蚜在三个小麦品种上取食行为的EPG比较[J]. 昆虫学报, 2007, 50(11): 1105-1110.
[28] Gabrys B, Tjallingii W F, Van Beek T A. Analysis of EPG recorded probing by cabbage aphid on host plant parts with different glucosinolate contents[J]. Journal of Chemical Ecology, 1997, 23(7): 1661-1673.
[29] 苗进, 武予清, 郁振兴, 等. 基于EPG的麦长管蚜、麦二叉蚜和禾谷缢管蚜食行为比较[J]. 生态学报, 2011,31(1): 175-182.
[30] 胡想顺,赵惠燕,胡祖庆,等. 麦长管蚜在3个小麦品种上取食行为的EPG比较[J]. 中国农业科学, 2008, 41(7): 1989-1994.
[31] Yin H D, Wang X Y, Xue K, et al. Impacts of transgenic Bt cotton on the stylet penetration behaviors of Bemisia tabaci biotype B: Evidence from laboratory experiments[J]. Insect science, 2010, 17(4): 344-352.
[32] Xue K, Wang X Y, Huang C H, et al. Stylet penetration behaviors of the cotton aphid Aphis gossypii on transgenic Bt cotton[J]. Insect Science, 2009, 16: 137-146.
[33] 朱俊庆. 不同茶树品种对假眼小绿叶蝉抗性的初步研究[J]. 植物保护学报, 1992, 19(1): 29-32.
[34] 张觉晚, 王沅江, 黄亚辉, 等. 茶树抗虫品种资源调查及抗性机制研究Ⅰ: 茶树品种对假眼小绿叶蝉抗性的筛选鉴定[J]. 茶叶通讯, 1994, 1: 2-5.
[35] 洪北边, 楼云芬. 茶树种质资源对假眼小绿叶蝉的抗性鉴定[J]. 中国茶叶, 1995, 5: 14-15.
[36] 曾莉, 王平盛, 许玫. 茶树对假眼小绿叶蝉的抗性研究[J]. 茶叶科学, 2001, 21(2): 90-93.
[37] 扈克明, 张艳梅, 王佳芳. 不同茶树品种间小绿叶蝉类群数量动态与抗虫性比较[J]. 茶叶科学, 2003, 23(1): 57-60.
[38] 王庆森, 陈常颂, 吴光远, 等. 黑刺粉虱对茶树品种的选择性[J]. 福建农林大学学报: 自然科学版, 2006, 35(3): 251-253.
[39] 苗进, 韩宝瑜. MeSA诱导茶树抗叶蝉取食效应的DC-EPG分析[J]. 植物保护学报, 2008, 35(2): 143-147.
[40] 洪北边, 楼云芬, 吕文明, 等. 茶资源抗病虫鉴定研究[G]//中国农业科学院茶叶研究所. 茶叶科学研究论文集(1991~1995). 上海: 上海科学技术出版社, 1996: 14-19.
[41] Gabrys B, Tjallingii W F, Van Beek T A. Analysis of EPG recorded probing by cabbage aphid on host plant parts with different glucosinolate contents[J]. Journal of Chemical Ecology, 1997, 23(7): 1661-1673.
[42] Klingler J, Powell G, Thompson G A, et al. Phloem specific aphid resistance in Cucumis melo line AR 5: effects on feeding behaviour and performance of Aphis gossypi[J]. Entomologia Experimentalis et Applicata, 1998, 86(1): 79-88.
[43] Lei H, Van Lenteren J C, Tjallingii W F. Analysis of resistance in tomato and sweet pepper against the greenhouse whitefly using electrically monitored and visually observed probing and feeding behaviour[J]. Entomologia Experimentalis et Applicata, 1999, 92(3): 299-309.
[44] Garzo E, Soria C, Gómez-Guillamón M L, et al. Feeding behavior of Aphis gossypii on resistant accessions of different melon genotypes(Cucumis melo)[J]. Phytoparasitica, 2002, 30(2): 129-140.
[45] Klingler J, Creasy R, Gao L, et al. Aphid resistance in Medicago trunculata involves antixenosis and phloem-specific, inducible antibiosis, and maps to a singlelocus flanked by NBS-LRR resistance gene analogs[J]. Plant Physiology, 2005, 137(4): 1445-1455.
[46] Alvarez A E, Tjallingii W F, Garzo E, et al. Location of resistance factors in the leaves of potato and wild tuber-bearing Solanum species to the aphid Myzus persicae[J]. Entomologia Experimentalis et Applicata, 2006, 121(2): 145-157.