利用SSR标记分析金观音（半）同胞茶树品种遗传差异

王让剑; 杨军; 孔祥瑞; 高香凤

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茶叶科学 ›› 2017, Vol. 37 ›› Issue (2) : 139-148.

利用SSR标记分析金观音（半）同胞茶树品种遗传差异

王让剑, 杨军, 孔祥瑞, 高香凤

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Genetic Analysis of Full- and Half-sib Families of Tea Cultivar Jinguanyin Based on SSR Molecular Markers

WANG Rangjian, YANG Jun, KONG Xiangrui, GAO Xiangfeng

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摘要

准确鉴定无性系茶树品种的遗传差异是对其进行保护与利用的重要前提。实验对金观音同胞及半同胞茶树品种的遗传差异进行了分析,结果表明,参试的31个SSR标记分型结果具有高度的稳定性。共扩增出117个等位位点,单个标记为2.0~8.0个,平均3.77个。基因型数为2.0~11.0个,平均5.16个。基因多样性指数范围为0.15~0.80,平均0.54。基因杂合度范围为0.17~0.94,平均0.61。引物多态信息含量范围为0.14~0.77,平均0.48。引物鉴别力范围为0.07~0.73,平均0.29。参试品种两两之间的遗传距离变化范围为0.10~0.52,平均0.35。遗传多样性大小顺序为：同胞茶树品种<半同胞茶树品种<非同胞茶树品种。利用系统发育树可将参试茶树品种分为5类,与茶树品种的叶色、制茶特征分类结果基本一致。任意两个品种同时在6个核心引物处（基因座）的基因型存在相同的可能性很低,为3.85×10^-5,这组核心引物具有较高的鉴别力,可将参试茶树品种完全鉴定。

Abstract

Accurate identification of the genetic differences among tea cultivars is the prerequisite for conservation and utilization. Genetic differences of the full- and half-sib families of tea cultivar Jinguanyin were analyzed based on SSR molecular markers. The results showed that the genotyping data of the tested 31 SSR markers were highly stable, with totally 117 alleles and averagely 3.77 alleles per marker (ranging from 2 to 8). The number of genotypes, gene diversity index, gene heterozygosity, polymorphism information content (PIC) and discrimination power (PID), genetic distance ranged from 2 to 11, 0.15 to 0.8, 0.17 to 0.94, 0.14 to 0.77, 0.07 to 0.73 and 0.10 to 0.52, averagely 5.16, 0.54, 0.61, 0.48, 0.29 and 0.35, respectively. The genetic diversity followed the order that full-sib tea cultivars ˂ half-sib tea cultivars ˂ non-sib tea cultivars. The tested tea cultivars could be divided into 5 categories by the phylogenetic tree, which was closely correlated with leaf colour and tea manufacture characteristics. It’s a low probability (3.85×10^-5) to identify the wrong cultivar using 6 core SSR markers, which were highly accurate in identification and could be used for fingerprinting analysis in the tested clonal tea cultivars.

导出引用

王让剑, 杨军, 孔祥瑞, 高香凤. 利用SSR标记分析金观音（半）同胞茶树品种遗传差异[J]. 茶叶科学. 2017, 37(2): 139-148

WANG Rangjian, YANG Jun, KONG Xiangrui, GAO Xiangfeng. Genetic Analysis of Full- and Half-sib Families of Tea Cultivar Jinguanyin Based on SSR Molecular Markers[J]. Journal of Tea Science. 2017, 37(2): 139-148

中图分类号： S571.1 Q52

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