高效液相色谱法检测茶叶中花青素类物质含量

茶叶科学 ›› 2025, Vol. 45 ›› Issue (3): 454-464.

高效液相色谱法检测茶叶中花青素类物质含量

叶水心^1,2, 王伟伟^2,3, 张梦雪^2,4, 江和源^2,3,*, 张海华^1,*

1.浙江农林大学食品与健康学院,浙江杭州 311300;
2.中国农业科学院茶叶研究所农业农村部特种经济动植物生物学与遗传育种重点实验室,浙江杭州 310008;
3.中国农业科学院都市农业研究所,四川成都 610299;
4.中国农业科学院研究生院,北京 100081

收稿日期:2024-12-24 修回日期:2025-01-20 出版日期:2025-06-15 发布日期:2025-06-18
通讯作者: *jiangheyuan@caas.cn;hhzhang@zafu.edu.cn
作者简介:叶水心,男,硕士研究生,主要从事茶食品方面的研究。
基金资助:
国家自然科学基金（32372766）、平阳县科技强农产业研究院产业提升项目（2023PYⅡ01）、浙江省科技计划项目（2023C02040）

Determination of Anthocyanin-like Substances in Tea by High Performance Liquid Chromatography

YE Shuixin^1,2, WANG Weiwei^2,3, ZHANG Mengxue^2,4, JIANG Heyuan^2,3,*, ZHANG Haihua^1,*

1. College of Food and Health, Zhejiang A & F University, Hangzhou 311300, China;
2. Key Laboratory of Special Economic Animal and Plant Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
3. Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610299, China;
4. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2024-12-24 Revised:2025-01-20 Online:2025-06-15 Published:2025-06-18

摘要/Abstract

摘要： 为建立茶叶中花青素类物质的高效液相色谱分析方法,以6种典型花青素（飞燕草色素、矢车菊色素、矮牵牛色素、天竺葵色素、芍药素和锦葵色素）为目标物,采用Cosmosil 5C₁₈-AR-Ⅱ色谱柱（250 mm×4.6 mm,5 μm）,流动相A为0.5%甲酸水溶液,流动相B为100%乙腈,柱温为35 ℃,流速为0.8 mL·min^-1,检测波长为525 nm,进样量为20 μL。在该分析条件下,各组分分离良好;在一定范围内,线性关系显著,决定系数均在0.999 0以上;重复性、精密度和稳定性试验的相对标准偏差（RSD）分别为1.20%~1.40%、1.15%~2.12%和1.35%~3.38%。加标回收率试验结果表明,花青素各组分平均回收率为86.08%~110.34%,RSD为0.24%~6.13%。该方法具有较好的重复性、精密度及稳定性,可用于检测茶叶中花青素类物质含量。

关键词: 花青素, 高效液相色谱法, 茶叶

Abstract: In order to establish a high performance liquid chromatographic (HPLC) method for the analysis of anthocyanin-like substances in tea, six typical anthocyanins (delphinidin, cyanidin, petunidin chloride, pelargonidin, paeonidin and malvidin) were used as the targets, and Cosmosil 5C₁₈-AR-Ⅱ chromatographic column (250 mm×4.6 mm, 5 μm) was used with the mobile phases of 0.5% formic acid in water and 100% acetonitrile as mobile phases A and B, respectively. The column temperature was 35 ℃, the flow rate was 0.8 mL·min^-1, the detection wavelength was 525 nm, and the injection volume was 20 μL. Under the analytical conditions, the anthocyanin components were well separated. In a certain range, the linear relationship was significant, and the correlation coefficient square (R²) was above 0.999 0. The relative standard deviations (RSD) of the repeatability, precision and stability tests were in the range of 1.20%-1.40%, 1.15%-2.12% and 1.35%-3.38%, respectively. The results of the spiked recovery test show that the average recoveries of the anthocyanin components were in the range of 86.08%-110.34% with an RSD between 0.24% and 6.13%. The method has good reproducibility, precision and stability, and can detect the contents of anthocyanin-like substances in tea.

Key words: anthocyanin, high performance liquid chromatography (HPLC), tea

中图分类号:

叶水心, 王伟伟, 张梦雪, 江和源, 张海华. 高效液相色谱法检测茶叶中花青素类物质含量[J]. 茶叶科学, 2025, 45(3): 454-464.

YE Shuixin, WANG Weiwei, ZHANG Mengxue, JIANG Heyuan, ZHANG Haihua. Determination of Anthocyanin-like Substances in Tea by High Performance Liquid Chromatography[J]. Journal of Tea Science, 2025, 45(3): 454-464.

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