大厂茶紫芽品系P113不同季节花青素调控相关基因表达分析

摘要/Abstract

摘要： 为明确大厂茶紫芽品系P113在不同季节花青素积累的分子机理及合成途径上相关基因的表达特点,对春、夏、秋3个季节的P113一芽二叶进行转录组（RNA-Seq）和代谢组（UHPLC-MS/MS）分析。结果显示,检测到的10种花青苷衍生物含量随季节变化,其中4种随春、夏、秋季节变化呈上调表达,与其叶色表现一致;结构基因PAL、C4H、CHS、F3'5'H表达模式基本一致,均在夏季上调表达,在秋季的表达量与夏季无显著差异;4CL酶基因获得的2个差异表达基因均在夏季上调表达;F3H、DFR、ANS表达模式相似,均在夏季上调表达,且各有1个基因在秋季呈现下调表达;2个F3'H基因中有1个在夏季下调表达,1个随季节变化上调表达;FLS的13个基因均在夏季上调表达,而在秋季呈现不同表达模式;修饰基因LAR在夏季和秋季均出现两种表达模式;ANR仅获得1个差异表达基因,随季节变化上调表达;3个UGT79B1基因中有1个在夏季下调表达,在秋季上调表达,另2个仅在夏季上调表达,在秋季的表达量与夏季无显著差异;获得的7个CCoAOMT差异表达基因均在夏季上调表达,其中2个在秋季下调表达;调控基因bHLH、MYB随季节变化上调表达,对花青素合成有正向调控作用。研究表明,大厂茶紫芽品系P113在不同季节结构基因、修饰基因和调控基因的表达具有一定的时间特性,导致了花青素积累差异。

关键词: 大厂茶, 花青素, 差异表达基因

Abstract: In order to clarify the molecular mechanism of anthocyanin accumulation and the expression characteristics of related genes in the synthetic pathway of anthocyanin in Camellia tachangensis F. C. Zhang purple bud line P113 in different seasons, one bud and two leaves of P113 in spring, summer and autumn were analyzed by RNA-Seq and UHPLC-MS/MS. The results show that the 10 anthocyanin contents changed with seasons, and 4 of them were up-regulated with the change of spring, summer and autumn, which were consistent with leaf color phenotype of ‘P113'. The expression patterns of structural genes PAL, C4H, 4CL, CHS, F3H and F3'5'H were basically the same, which were up-regulated in summer, but not significantly changed in autumn. The two differentially expressed 4CL genes were up-regulated in summer. The gene expression patterns of F3H, DFR and ANS were similar, and their expressions were up-regulated in summer and down-regulated in autumn. One of the two F3'H genes was down-regulated in summer, and one was up-regulated with seasonal changes. The 13 genes of FLS were all up-regulated in summer, but they showed different expression patterns in autumn. The modifier gene LAR had two expression patterns in summer and autumn. There was only 1 differentially expressed gene in ANR that was up-regulated with seasonal changes. Among the three UGT79B1 genes, one was down-regulated in summer, up-regulated in autumn, and the rest two genes were up-regulated in summer, but not significantly changed in autumn. Seven differentially expressed CCoAOMT genes were up-regulated in summer. Two of them down regulated in autumn, and the rest five genes were not significant changed. The regulatory genes bHLH and MYB were up-regulated with seasonal changes, and had a positive relationship with anthocyanin biosynthesis. The results show that the expressions of structural genes, modifier genes and regulatory genes of ‘P113' (Camellia tachangensis F. C. Zhang) had certain time characteristics in different seasons, which led to the differences in anthocyanin accumulation.

Key words: Camellia tachangensis F. C. Zhang, anthocyanins, differentially expressed genes

中图分类号:

S571.1

刘霞, 李芳, 宋勤飞, 牛素贞, 吕立堂. 大厂茶紫芽品系P113不同季节花青素调控相关基因表达分析[J]. 茶叶科学, 2021, 41(6): 789-801.

LIU Xia, LI Fang, SONG Qinfei, NIU Suzhen, LYU Litang. Analysis of Gene Expression Related to Anthocyanin Regulation of ‘P113' Purple Strain of Camellia tachangensis F. C. Zhang in Different Seasons[J]. Journal of Tea Science, 2021, 41(6): 789-801.

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