茶叶科学 ›› 2019, Vol. 39 ›› Issue (5): 506-520.doi: 10.13305/j.cnki.jts.2019.05.002
黄丹娟1, 谭荣荣1, 陈勋1, 王红娟1, 龚自明1, 王友平2, 毛迎新1, *
收稿日期:
2019-05-21
修回日期:
2019-06-28
出版日期:
2019-10-15
发布日期:
2019-10-15
通讯作者:
*maoyingxin@126.com
作者简介:
黄丹娟,女,助理研究员,主要从事茶树栽培生理研究,E-mail: huangdjtea@163.com。
基金资助:
HUANG Danjuan1, TAN Rongrong1, CHEN Xun1, WANG Hongjuan1, GONG Ziming1, WANG Youping2, MAO Yingxin1, *
Received:
2019-05-21
Revised:
2019-06-28
Online:
2019-10-15
Published:
2019-10-15
摘要: 探讨茶树响应铝(Aluminum,Al)的基因调控网络和表达模式,确定一些关键候选基因,为茶树耐Al分子机制研究奠定基础。测定了0、0.2、1、2、4βmmol·L-1 5个Al3+浓度处理7βd的福鼎大白茶根系抗氧化酶活性和Al含量变化,并提取0βmmol·L-1(R0)、1βmmol·L-1(R1)和4βmmol·L-1(R4)3个浓度下的茶树根系总RNA,通过Illumina Hiseq Xten平台进行高通量转录组测序。结果表明,随着Al3+浓度的升高,根系POD(Peroxidase,过氧化物酶)活性逐渐下降,APX(Ascorbic acid peroxidase,抗坏血酸过氧化物酶)活性则逐渐升高。SOD(Superoxide dismutase,超氧化物歧化酶)活性在Al3+浓度为1βmmol·L-1时最高,CAT(Catalase,过氧化氢酶)活性在各处理间无显著差异。根系中Al含量随着Al3+浓度的升高呈先上升后下降趋势,在Al3+浓度为1βmmol·L-1时达到最高。经筛选得到R1 VS R0,R4 VS R0,R4 VS R1的DEGs(Differentially expressed genes)分别为1β894、2β439个和1β384个,显著上调(下调)的差异表达基因分别有733(1β161)、846(1β593)个和628(756)个。GO富集分析表明,3个处理组在生物学途径中富集最多的类别均为刺激响应。在分子功能和细胞组件方面,R1 VS R0和R4 VS R0富集最多的类别均为核酸结合转录因子活性和细胞外围,R4 VS R1富集最多的类别为氧化还原酶活性相关基因和膜区域。KEGG富集分析表明,R1 VS R0、R4 VS R0、R4 VS R1分别显著富集了29、41条和19条Pathway,它们包括转录因子、转运蛋白、植物-病原菌互作、苯丙烷生物合成途径等,鉴定到多个参与调控活性氧代谢、有机酸或金属转运蛋白、转录因子及细胞壁结构修饰等生理过程的基因在Al诱导后上调或抑制表达,显示这些基因与茶树耐Al分子机制密切相关。
中图分类号:
黄丹娟, 谭荣荣, 陈勋, 王红娟, 龚自明, 王友平, 毛迎新. 铝诱导的茶树根系转录组变化分析[J]. 茶叶科学, 2019, 39(5): 506-520. doi: 10.13305/j.cnki.jts.2019.05.002.
HUANG Danjuan, TAN Rongrong, CHEN Xun, WANG Hongjuan, GONG Ziming, WANG Youping, MAO Yingxin. Transcriptome Analysis of Root Induced by Aluminum in Tea Plants (Camellia sinensis)[J]. Journal of Tea Science, 2019, 39(5): 506-520. doi: 10.13305/j.cnki.jts.2019.05.002.
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