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Journal of Tea Science ›› 2004, Vol. 24 ›› Issue (1): 60-64.doi: 10.13305/j.cnki.jts.2004.01.012

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Analysis of the Xanthophyll Cycle Component in Fresh Leaves of Tea Plant by High Performance Liquid Chromatography and Its Photo-protection

WEI Chao-ling1, 2, JIANG Chang-jun1, TAO Han-zhi1, HU Ying-hui1   

  1. 1. Key Laboratory of Tea Biotechnology, Ministry of Agriculture, Anhui Agricultural University ;
    2. Department of Agronomy, Anhui Agricultural University, Hefei 230036, China
  • Received:2003-02-18 Revised:2003-07-12 Online:2004-03-25 Published:2019-09-11

Abstract: The xanthophyll cycle components in fresh leaves of tea plant (Camellia sinensis cv. Fudingdabai)were extracted by acetone. The pigments were analyzed with a Spherisorb column (C18 , 5μm,250㎜×4.6βmm),and were eluted using acetonitrile: methanol(85:15) and methanol:ethyl acetate(68:32) by a linear gradient. The pigments were detected by their absorbance at 445βnm. It was showed that violaxanthin, antheraxanthin and zeaxanthin from fresh leaves of tea plant were detected by HPLC, respectively, under temperatures of 15℃, 22℃ and 30℃, meanwhile, the pigments were separated well, and it is a sensitive, well-reappeared method. Tea plant had a larger xanthophyll cycle pool and a larger de-epoxidation state in higher sun-light, which indicated xanthophyll cycle plays an important role in photo-protection in tea plant.

Key words: High performance liquid chromatography, Tea plant, Xanthophylls cycle component, Photo-protection

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