利用cDNA-AFLP技术研究茶树花蕾发育基因差异表达片段

doi:10.13305/j.cnki.jts.2007.03.007

Abstract

Abstract: For studies of differential gene expression, cDNA-AFLP was analyzed in tea plant at the stage of bud flower development. The result showed that there were evident differential expression in flower between the early stage and the late stage. Of the 1110 fragments inspected, 35 and 87 specially displayed in the early and late stages; 15 fragments were cloned and sequenced. Sequence analysis indicated that among 15 squenced fragments, nine were sequence-similar to proteins in GenBank, i.e. oxidoreductase, chromosome 13, 14-3-3 protein, Amyrel gene, calreticulin interacted protein, ATP sulfurylase, catalytic / iron ion binding, desiccation-related protein and ntp302, two were similar to hypothetical proteins, and no similar sequence were found in GenBank for the other 4 fragment.

Key words: bud flower development, cDNA-AFLP, tea plant(Camellia sinensis(L.)O. Kuntze cv. Wulong), gene differential expression

CLC Number:

S571.1
Q523

YU Mei, JIANG Chang-jun, YE Ai-hua, WANG Zhao-xia, ZHU Lin, DENG Wei-wei, GAO Xuan, WAN Xiao-chun. Analysis of Differential Gene Expression of Flower bud of Tea Plant (Camellia sinensis) by cDNA-AFLP[J]. Journal of Tea Science, 2007, 27(3): 259-264.

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Analysis of Differential Gene Expression of Flower bud of Tea Plant (Camellia sinensis) by cDNA-AFLP

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