Analysis of Differential Expression Genes in Cold-induced Tea Plant

ZOU Zhong-wei; FANG Wan-ping; ZHANG Ding; DUAN Yun-shang; LI Xing-hui

doi:10.13305/j.cnki.jts.2008.04.011

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Journal of Tea Science ›› 2008, Vol. 28 ›› Issue (4) : 249-254. DOI: 10.13305/j.cnki.jts.2008.04.011

Analysis of Differential Expression Genes in Cold-induced Tea Plant

ZOU Zhong-wei, FANG Wan-ping^*, ZHANG Ding, DUAN Yun-shang, LI Xing-hui^*

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Abstract

The differential expression was investigated between cold-induced and normal environment in tea plant (Camellia sinensis) with cDNA-AFLP (cDNA Amplified Fragments Length Polymorphism). Expression profiles were generated around 86 special cDNA fragments (16 pairs primer, 256 primer combinations). Ten differentially expressed transcript-derived fragments were isolated. Cha1 revealed similarities (77%) to one kind of low temperature and salt responsive protein in Arabidopsis thaliana. Cha5 revealed similarities (89%) to Arabidopsis thaliana 60 S ribosomal protein L7 (RPL7B), ChaH8 had high similarities (79%) to Nicotiana tabacum A10 mRNA for stress-inducible H1 histone-like protein. Cha9 had high similarities to Populus EST from severe drought-stressed opposite wood. Other 6 fragments showed no homology to the known sequences in GenBank, perhaps they were new genes.

Key words

cDNA-AFLP / cold-stress / expression / tea plant (Camellia sinensis)

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ZOU Zhong-wei, FANG Wan-ping, ZHANG Ding, DUAN Yun-shang, LI Xing-hui. Analysis of Differential Expression Genes in Cold-induced Tea Plant[J]. Journal of Tea Science. 2008, 28(4): 249-254 https://doi.org/10.13305/j.cnki.jts.2008.04.011

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