低温胁迫下茶树基因表达的差异分析

doi:10.13305/j.cnki.jts.2008.04.011

Abstract

Abstract: The differential expression was investigated between cold-induced and normal environment in tea plant (Camellia sinensis) with cDNA-AFLP (cDNA Amplified Fragments Length Polymorphism). Expression profiles were generated around 86 special cDNA fragments (16 pairs primer, 256 primer combinations). Ten differentially expressed transcript-derived fragments were isolated. Cha1 revealed similarities (77%) to one kind of low temperature and salt responsive protein in Arabidopsis thaliana. Cha5 revealed similarities (89%) to Arabidopsis thaliana 60 S ribosomal protein L7 (RPL7B), ChaH8 had high similarities (79%) to Nicotiana tabacum A10 mRNA for stress-inducible H1 histone-like protein. Cha9 had high similarities to Populus EST from severe drought-stressed opposite wood. Other 6 fragments showed no homology to the known sequences in GenBank, perhaps they were new genes.

Key words: cDNA-AFLP, tea plant (Camellia sinensis), cold-stress, expression

CLC Number:

S571.1
Q523

ZOU Zhong-wei, FANG Wan-ping, ZHANG Ding, DUAN Yun-shang, LI Xing-hui. Analysis of Differential Expression Genes in Cold-induced Tea Plant[J]. Journal of Tea Science, 2008, 28(4): 249-254.

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Analysis of Differential Expression Genes in Cold-induced Tea Plant

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