利用cDNA-AFLP技术分析炭疽菌危害诱导茶树的差异表达基因

doi:10.13305/j.cnki.jts.20200117.001

Abstract

Abstract: Differentially expressed genes related to Camellia sinensis cv. Maoxie against Colletotrichum sp.1 infection were screened by cDNA-AFLP (cDNA-amplified fragment length polymorphism) to provide reference for further illustrating the molecular mechanism underlying tea resistance to anthracnose. A total of 256 pairs of selective primers were applied to amplify the cDNA of leaves collected at 0 h and 48 h after Colletotrichum infection, and 136 differentially expressed transcript-derived fragments (TDFs) were obtained via screening, sequencing and BLAST analysis. The analysis of the homologous genes revealed that 128 TDFs were homologous with the known genes in Nr database. Most of them were related to stress responses, biological regulation and signal transduction, and 51 TDFs were highly homologous to differential expressed genes of tea plant under cold, drought or salt conditions in TPIA database. Further qRT-PCR analysis of 27 TDFs showed that the expression profiles of 24 TDFs were consistent with those of cDNA-AFLP results, among which several resistance-related genes including WRKY transcription factor, ethylene-responsive transcription factor, peroxidase and superoxide dismutase were significantly up-regulated. The differential expressed genes found in this study would lay a foundation for further analysis of their functions.

Key words: Camellia sinensis, Colletotrichum sp.1, cDNA-AFLP, differential expression genes

CLC Number:

WEI Rifeng, LAI Jiandong, PENG Chengbin, ZHANG Chengkang, LIAN Lingli, LIU Wei. cDNA-AFLP Reveals Differential Gene Expression Profiles of Tea Plant (Camellia sinensis cv. Maoxie) Induced by Colletotrichum sp.1 Infection[J]. Journal of Tea Science, 2020, 40(1): 26-38.

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cDNA-AFLP Reveals Differential Gene Expression Profiles of Tea Plant (Camellia sinensis cv. Maoxie) Induced by Colletotrichum sp.1 Infection

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