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Journal of Tea Science ›› 2020, Vol. 40 ›› Issue (1): 26-38.doi: 10.13305/j.cnki.jts.20200117.001

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cDNA-AFLP Reveals Differential Gene Expression Profiles of Tea Plant (Camellia sinensis cv. Maoxie) Induced by Colletotrichum sp.1 Infection

WEI Rifeng1, LAI Jiandong1,4, PENG Chengbin1, ZHANG Chengkang2, LIAN Lingli3,*, LIU Wei1,2,*   

  1. 1.College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    2. Ningde Normal University, Ningde 352100, China;
    3. College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    4. Hubei University of Science and Technology, Xianning 437100, China
  • Received:2019-04-02 Revised:2019-06-04 Online:2020-02-15 Published:2020-02-04

Abstract: Differentially expressed genes related to Camellia sinensis cv. Maoxie against Colletotrichum sp.1 infection were screened by cDNA-AFLP (cDNA-amplified fragment length polymorphism) to provide reference for further illustrating the molecular mechanism underlying tea resistance to anthracnose. A total of 256 pairs of selective primers were applied to amplify the cDNA of leaves collected at 0 h and 48 h after Colletotrichum infection, and 136 differentially expressed transcript-derived fragments (TDFs) were obtained via screening, sequencing and BLAST analysis. The analysis of the homologous genes revealed that 128 TDFs were homologous with the known genes in Nr database. Most of them were related to stress responses, biological regulation and signal transduction, and 51 TDFs were highly homologous to differential expressed genes of tea plant under cold, drought or salt conditions in TPIA database. Further qRT-PCR analysis of 27 TDFs showed that the expression profiles of 24 TDFs were consistent with those of cDNA-AFLP results, among which several resistance-related genes including WRKY transcription factor, ethylene-responsive transcription factor, peroxidase and superoxide dismutase were significantly up-regulated. The differential expressed genes found in this study would lay a foundation for further analysis of their functions.

Key words: Camellia sinensis, Colletotrichum sp.1, cDNA-AFLP, differential expression genes

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