Studies on Polyphenol-resisting Characteristics of Several Kinds of Agrobacterium and the Demestication of Polyphenol-resisting Strain

CANG Mei-qin; ZHOU Jian; CHENG Hao; LI Xing-hui; WANG Li-yuan

doi:10.13305/j.cnki.jts.2008.03.004

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Journal of Tea Science ›› 2008, Vol. 28 ›› Issue (3) : 189-194. DOI: 10.13305/j.cnki.jts.2008.03.004

Studies on Polyphenol-resisting Characteristics of Several Kinds of Agrobacterium and the Demestication of Polyphenol-resisting Strain

CANG Mei-qin^1,2, ZHOU Jian², CHENG Hao^2,*, LI Xing-hui^1,*, WANG Li-yuan²

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Abstract

In order to make clear the variation of polyphenol-resisting characteristics of different kinds of Agrobacterium as well as the potential in improving the polyphenol-resisting ability and the Agrobacterium-mediated genetic transformation rate by domestication culture, the inhibition effect of tea polyphenols on four kinds of Agrobacterium strains, the polyphenol-resisting demestication of Agrobacterium strains and the improvement of genetic transformation ability in demesticated polyphenol-resisting strain were studied. The results showed that tea polyphenols had obvious inhibition on the growth of different kinds of Agrobacterium, but it varied greatly among these studied Agrobacterium. A polyphenol-resisting strain EHA105AP was obtained by domestication culture procedure, the polyphenol-resisting ability of which enhanced 90.89% compared with that of the original. Meanwhile, the rate of transient expression of GUS gene induced by the domesticated strain increased 43.85% and 57.55% respectively in callus and leaf explants case.

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agrobacterium / polyphenol-resisting strain / tea polyphenols / transient expression of GUS gene

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CANG Mei-qin, ZHOU Jian, CHENG Hao, LI Xing-hui, WANG Li-yuan. Studies on Polyphenol-resisting Characteristics of Several Kinds of Agrobacterium and the Demestication of Polyphenol-resisting Strain[J]. Journal of Tea Science. 2008, 28(3): 189-194 https://doi.org/10.13305/j.cnki.jts.2008.03.004

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