Metabonomic Analysis on the Effects of different Nitrogen Application Rates and Light/Dark Change on Metabolism in Tea Leaves

YANG Yi-yang; MA Li-feng; LI Xing-hui; R. G. Ratcliffe; N. J. Kruger; RUAN Jian-yun

doi:10.13305/j.cnki.jts.2013.06.008

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Journal of Tea Science ›› 2013, Vol. 33 ›› Issue (6) : 491-499. DOI: 10.13305/j.cnki.jts.2013.06.008

Metabonomic Analysis on the Effects of different Nitrogen Application Rates and Light/Dark Change on Metabolism in Tea Leaves

YANG Yi-yang^1,2, MA Li-feng², LI Xing-hui³, R. G. Ratcliffe⁴, N. J. Kruger⁴, RUAN Jian-yun^{2, *}

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Abstract

Amino acids, polyphenols, caffeine and other metabolites such as sugars and aromatic substances are important determinants of tea quality. Different cultivation practices affect tea quality by altering the basic metabolite profiles. In this study, the metabolomic changes through nitrogen and samples collected in day and night were investigated in fresh tea shoots by ¹H-NMR for non-targeted analysis coupled with a multivariate data analysis and by HPLC for targeted analysis to reveal the mechanism of formation, transformation and relationship of the major characteristic quality components in fresh tea leaves. Non-targeted analysis with ¹H-NMR showed significant effect of samples collected in day and night but no difference among N application rates. However, targeted analysis with HPLC showed both significant variations in amino acids and catechins as affected by N rates and light/dark change. There were higher sensitivity of amino acids and sugars but lower sensitivity of tea polyphenols through the metabolomic analysis using ¹H-NMR.

Key words

Camellia sinensis / amino acids / light/dark / nitrogen application rate / polyphenols

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YANG Yi-yang, MA Li-feng, LI Xing-hui, R. G. Ratcliffe, N. J. Kruger, RUAN Jian-yun. Metabonomic Analysis on the Effects of different Nitrogen Application Rates and Light/Dark Change on Metabolism in Tea Leaves[J]. Journal of Tea Science. 2013, 33(6): 491-499 https://doi.org/10.13305/j.cnki.jts.2013.06.008

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