合成茶树咖啡碱相关的N-甲基转移酶基因家族的克隆及序列分析

doi:10.13305/j.cnki.jts.2014.02.015

Abstract

Abstract: Caffeine is an important bioactive compound in tea. It is synthesized from xanthosine catalysed by N-methyltransferase (NMT) that using S-adenosylmethionine (SAM) as the methyl donor. Based on the characterization of high similarity of CS (Caffeine Synthase) gene sequences, 6 NMTs were cloned from Baiye 1 by using the technique of long PCR and cloning flanking DNA sequence. Two of them are TCS1 and TCS2, one is a pseudogene, and the other three are named TCS3, TCS4 and TCS5, respectively. It was found that these genes were all composed by four exons and three introns through analysis of gene structure. The NMTs of Camellia plants were clustered into 5 groups, the similarity of TCS4 and TCS5 between with other genes is lower. These results provided useful references to dissect the genetic mechanism of caffeine from genome level in tea plant for the future.

Key words: tea plant, N-methyltransferase, gene family, cloning

CLC Number:

JIN Jiqiang, YAO Mingzhe, MA Chunlei, MA Jiangqiang, CHEN Liang. Cloning and Sequence Analysis of the N-methyltransferase Gene Family Involving in Caffeine Biosynthesis of Tea Plant[J]. Journal of Tea Science, 2014, 34(2): 188-194.

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Cloning and Sequence Analysis of the N-methyltransferase Gene Family Involving in Caffeine Biosynthesis of Tea Plant

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