Cloning of CsCHLI Gene and Its Expression Analysis in Different Albino Tea Cultivars (Camellia sinensis)

ZHAO Yiqing; LIU Zhengjun; ZHANG Tianxin; ZHAO Yanting; XIAO Bin; GAO Yuefang

doi:10.13305/j.cnki.jts.20210224.001

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Journal of Tea Science ›› 2021, Vol. 41 ›› Issue (3) : 327-336. DOI: 10.13305/j.cnki.jts.20210224.001

Research Paper

Cloning of CsCHLI Gene and Its Expression Analysis in Different Albino Tea Cultivars (Camellia sinensis)

ZHAO Yiqing¹, LIU Zhengjun¹, ZHANG Tianxin², ZHAO Yanting¹, XIAO Bin¹, GAO Yuefang^1,*

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Abstract

Magnesium chelatase is one of the key enzymes in the process of chlorophyll synthesis, which is closely related to the albino phenomenon of leaves. In this study, the leaves of tea cultivars Shaancha 1, Baiye 1, Jibai 1, Huangjinya and Haungjinye were used as materials to clone the full-length CDS sequences of CsCHLI (Mg-chelatase I subunit). Multi-sequence alignment shows that the two base differences (G502C, C1169T) were found in Baiye 1, which lead to the change of two amino acid residues (G168R, A390V) in the AAA⁺ and AAA lid domain. Meanwhile, there was an amino acid residue changed (R401H) in the AAA lid domain of Huangjinye, due to the difference of G1202A. Subcellular localization analyses illustrates that CsCHLI is localized in chloroplast and the differences of the bases or amino acid residues did not affect its subcellular localization. The RNA secondary structure analysis shows that base site mutations of CsCHLI would affect its stem-loop structure and the stability. Moreover, the chlorophyll contents in albino tea plants were 25%-77% of that in Shaancha 1, and the expressions of CsCHLI in albino tea plants were 24%-46% of that in Shaancha 1. The results show that the CsCHLI expression was positively correlated with chlorophyll content in tea plants. Furthermore, these results provided experimental evidences for the in-depth study of the chlorophyll metabolism in albino tea plants.

Key words

CsCHLI / Camellia sinensis / chlorophyll content / RNA secondary structure / subcellular localization

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ZHAO Yiqing, LIU Zhengjun, ZHANG Tianxin, ZHAO Yanting, XIAO Bin, GAO Yuefang. Cloning of CsCHLI Gene and Its Expression Analysis in Different Albino Tea Cultivars (Camellia sinensis)[J]. Journal of Tea Science. 2021, 41(3): 327-336 https://doi.org/10.13305/j.cnki.jts.20210224.001

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