Preparation of Guangyuan Yellow Tea Self-Assembled Zinc Nanocomposite and Their Antibacterial and Anti-Inflammatory Activities

AN Lujing; QIAN Yiyue; CHANG Ke; TIAN Baoming; MU Dan; ZHANG Mingzhu; CHEN Hongping; ZHANG Xiangchun

doi:10.13305/j.cnki.jts.2026.01.005

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Journal of Tea Science ›› 2026, Vol. 46 ›› Issue (1) : 139-150. DOI: 10.13305/j.cnki.jts.2026.01.005

Preparation of Guangyuan Yellow Tea Self-Assembled Zinc Nanocomposite and Their Antibacterial and Anti-Inflammatory Activities

AN Lujing^1,2, QIAN Yiyue³, CHANG Ke^1,2, TIAN Baoming², MU Dan¹, ZHANG Mingzhu^1,*, CHEN Hongping², ZHANG Xiangchun^2,*

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Abstract

Currently, few studies have reported on the direct use of tea extracts as raw materials for creating novel functional composites. This study successfully synthesized Guangyuan Yellow Tea-Zinc Nanocomposites (Y-Zn) using Guangyuan Yellow Tea infusion and zinc sulfate as raw materials through a green, simple one-step self-assembly method. Characterization via UV-vis spectrophotometry, transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS) reveals that the synthesized nanocomposite exhibits uniform particle size and excellent dispersion. Antibacterial experiments demonstrated that Y-Zn exhibits dose-dependent antibacterial effects against multiple Gram-positive bacteria, including drug-resistant strains, and effectively inhibits bacterial biofilm formation. Antioxidant experiments revealed its excellent free radical scavenging ability, achieving over 80% scavenging efficiency at a concentration of 20 μg·mL^-1. Cellular experiments confirmed that Y-Zn suppresses lipopolysaccharide (LPS)-induced macrophage inflammatory responses by downregulating inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression. Furthermore, zebrafish models demonstrated its favorable biosafety profile. In summary, this study directly utilized tea infusion as the subject to develop a self-assembled composite that possesses multiple biological activities and safety, providing insights into the application of China's abundant tea resources in the field of life sciences and health.

Key words

antibacterial activity / anti-inflammatory activity / nanocomposite / self-assembly / tea infusion

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AN Lujing, QIAN Yiyue, CHANG Ke, TIAN Baoming, MU Dan, ZHANG Mingzhu, CHEN Hongping, ZHANG Xiangchun. Preparation of Guangyuan Yellow Tea Self-Assembled Zinc Nanocomposite and Their Antibacterial and Anti-Inflammatory Activities[J]. Journal of Tea Science. 2026, 46(1): 139-150 https://doi.org/10.13305/j.cnki.jts.2026.01.005

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